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Showing 1 to 18 of 18 for “"T6SS"”.
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Structure of T6SS components and assessment of potential Gram-ve drug targets
The structure and potential function of two components of the type VI secretion system in Serratia marcescens have been investigated by X-ray crystallography. Chapters 1 and 2 describe the background of the type VI secretion system and experimental methods respectively. In chapter 3, the solution …
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Characterizing the Role of the Type VI Secretion System in Interbacterial Species Interactions and Pathogenesis
… adapted is the use of type VI secretion systems (T6SS). The T6SS is a large needle complex that spans across the entire bacterial cell wall and functions through a contraction mechanism that results in the delivery of toxic effector proteins into neighbouring cells. Virulence is achieved through …
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An analysis of selected secretion systems of Pseudomonas species
… System (T3SS) and the Type VI Secretion System (T6SS) systems of Pseudomonas, a widespread genus including clinical pathogens and biocontrol strains. Bioinformatic analysis of T6SS phylogeny and associated gene content within Pseudomonas identified several T6SS phylogenetic groups, and linked …
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Characterization of the type VI protein secretion system encoded in the Salmonella pathogenicity island 19 and its role in the pathogenicity of serotypes Gallinarum and Enteritidis
… bacteriana. El Sistema de Secreción Tipo VI (T6SS) es un nuevo mecanismo de translocación de proteínas que existe en la mayoría de bacterias Gram-negativo que se encuentran en contacto íntimo con células eucariontes, incluyendo a aquellas que son patógenos humanos y de plantas. El papel …
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Dissecting the puncturing device of the Type VI Secretion System: role of Hcp, VgrG and PAAR proteins in assembly and secretion of effectors
The Type VI secretion system (T6SS) is a macromolecular machinery, widespread in Gram negative bacteria, which is used to deliver toxic proteins (“effectors”) directly into target eukaryotic or prokaryotic cells. As a result, this system plays a crucial role both in direct pathogenicity and in …
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Specialised Effectors and Accessory Proteins Important in the Antibacterial Type VI Secretion System of <i>Serratia marcescens</i>
… and mechanism of the Type VI secretion system (T6SS). The T6SS is widespread in Gram-negative bacteria and can deliver toxins (‘effectors’) into both eukaryotic host cells and rival bacterial cells. Effector proteins are believed to be delivered by either covalent association ('specialised' …
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Investigation into the transcriptional regulation and role in virulence of the Type VI Secretion System of <em>Serratia marcescens</em> Db10
The Type 6 Secretion system (T6SS) is the most recently identified secretion system in Gram-negative bacteria. It is widely distributed and has been found to target both eukaryotic and prokaryotic cells. The T6SS has been described as resembling an inverted bacteriophage cell puncturing device …
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Characterizing the advantages of diverse effector secretion in Pseudomonas aeruginosa
… such pathway is the type VI secretion system (T6SS) which is broadly distributed in Gram-negative bacterial species. The T6SS antagonizes nearby cells by directly injecting them with toxic antimicrobial proteins, termed effectors. Effectors target conserved, essential structures in the cell, …
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Important role of the type VI secretion system in the virulence of an emergent human pathogen Aeromonas hydrophila: Delineating the mechanism of action of its effectors
… characterized the new type 6 secretion system (T6SS) from isolate SSU of A. hydrophila and demonstrated its role in bacterial virulence. We have provided evidence that the T6SS is independent of other secretion systems and that the vasH gene is essential for the expression of the genes encoding …
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Characterization of Novel Type VI Effectors of Acidovorax citrulli and Their Applicability to Biological Control of Plant Diseases
… secretion systems, the Type VI Secretion System (T6SS), a nanomachine widely distributed in Gram-negative bacteria, is gaining increasing attention due to its involvement in microbe-microbe and microbe-host interactions through secreting toxins into host cells, microbial competitors, and the …
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Exploring Interactions of Phyllosphere Epiphytes with Plant Pathogenic Bacteria Pseudomonas and Xanthomonas on Tomato
… X. <em>perforans</em> type VI secretion system (T6SS) mutant strains to observe the role of type VI secretion system in infection with a potential biocontrol agent. To explain interactions among the <em>phyllosphere</em> residents, nutritional similarity, motility and direct inhibition were …
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The role of the Vibrio cholerae Type VI secretion system during kin cell interaction, biofilm formation and priority effects
… cholerae’s </em>Type VI secretion system (T6SS) is one of the arsenals used by the bacteria for its long-term survival in different environments, against a variety of opponents, and for its maintained pathogenicity in vulnerable regions of the world. Therefore, the more we understand about …
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Analysis of the diverse antibacterial strategies used by <i>Serratia marcescens</i> Db10
… Type VI Secretion System (T6SS) is the most recently identified Gramnegative bacterial secretion system and has been demonstrated to play important roles in mediating contact dependent interactions with neighbouring bacterial and eukaryotic cells. <i>S. marcescens</i> Db10 …
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CHARACTERIZATION OF CA²⁺-MEDIATED VIRULENCE AND MICROBIAL COMPETITION IN THE PLANT PATHOGEN PSEUDOMONAS SYRINGAE
… the activity of type VI secretion system (T6SS)-dependent competition. I found that calcium enhances T6SS-mediated killing against E.coli. Further analyses implicated GacAS plays a key role in integrating calcium signals to modulate T6SS activity. By dissecting the regulatory networks and …
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Combating Plague: Fighting a War on Two Fronts Through Vaccine Design and New Therapeutic Intervention
… components. Through the generation of several T6SS associated gene deletion mutants, we have shown significant attenuation of the bacterium in pneumonic models of plague. Combinatorial deletions of T6SS associated genes resulted in further augmenting attenuation as well as providing protection …
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Identification of the virulence determinants of the neonatal meningitic bacterium Cronobacter sakazakii
… activator (Cpa) ,and type VI secretion (T6SS) gene cluster. The majority of C. sakazakii strains were serum resistant (32/36), and thus, they had the ability to survive in blood by preventing serum-mediated killing. Also, different iron acquisition systems were encoded by C. sakazakii 97% …
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Evolution and Regulation of Virulence and Antimicrobial Resistance in Pseudomonas aeruginosa
… genes were upregulated, including the H2-T6SS and the mexGHI-opmD efflux pump. Genomics analysis of mutations in mexT in P. aeruginosa genomes showed that those are prevalent amongst clinical and environmental isolates and not restricted to a particular region of the gene. My findings …
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Evidence of Water to Human Transmission of Aeromonas Hydrophila: Critical Role of Quorum Sensing in Bacterial Virulence & Host Response
Aeromonas hydrophila is a Gram-negative bacterium which produces a wide variety of virulence factors leading to both intestinal and extra-intestinal infections in humans. A large number of water and clinical Aeromonas isolates were molecularly finger-printed and our data provided the evidence of …