Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"T47D"”.
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The effect of distinct BRCA1 splice forms on drug sensitivity in breast- and ovarian cancer cell lines
… study was to knock out BRCA1 expression in the T47D breast cancer cell line and the CAOV3 ovarian cancer cell line and overexpress longer and shorter transcripts of BRCA1 in the cells. Subsequently, the aim was to assess their sensitivity to PARP inhibition and thereafter, study how each BRCA1 …
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Mechanismen der Durchbrechung der sekundären Antiöstrogenresistenz durch GnRH-Analoga in Mammakarzinomzellen
… parentalen Mammakarzinomzellen MCF 7 und T47D und ihren Tamoxifen-resistenten Sublinien MCF-7-TR und T47D-TR etabliert. Analoga von Gonadotropin-Releasing Hormon (GnRH)-I und -II stellen die Wirksamkeit von Tamoxifen in diesen Zellen wieder her. In der vorliegenden Arbeit sollten nun die …
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Antiproliferative activities of Euphorbia bicolor (euphorbiaceae) and Maclura pomifera (moraceae) extracts on estrogen receptor-positive and triple-negative breast cancer cell lines
… effects in estrogen receptor positive MCF-7 and T47D breast cancer cells. Extracts of both male and female branches and leaves (500 µg/mL) reduced cancer cell viability by 75–80%. These effects were mediated by intracellular calcium overload via TRPV1 activation, leading to apoptosis. Blocking …
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Context Dependent Roles of MDMX (MDM4) and MDM2 in Breast Cancer Proliferation and Circulating Tumor Cells
… MDM2 strongly promotes proliferation in ER+ T47D breast cancer cells in a p53-independent manner. Whether MDM2 elicits <em>in vivo</em> p53-independent proliferative functions in T47D breast cancer cells has not been determined. Furthermore it has been shown that ectopic expression of MDM2 …
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Hormonal modulation of sex steroid hormone receptors and oncogenes in human breast cancer cells
… levels by 50% but did not change HER-2/neu. In T47D cells, which contain low ER and high PR levels, E$\sb2$ induced ER mRNA 2.5-fold in 2d. In contrast, R5020 reduced both ER mRNA and PR mRNA levels to 20% of control in 2d. RU486 caused an initial 50% drop by 6h followed by a rise to control …
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Novel tools to identify estrogen regulated genes important for breast and ovarian cancer cell proliferation
… stimulate near maximal proliferation of MCF-7, T47D, T47D ER D538G, BG1 and PEO4 breast and ovarian cancer cells. Furthermore, picomolar E2 stimulates robust colony formation in anchorage independent soft agar assays. This effect is not primarily mediated through ER 's extra-nuclear activation …
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Bst2 Isoforms: Distribution and Potential Use In Cancer Therapeutics
… BST2-targeting short hairpin RNA (shRNA) in the T47D breast cancer cell line. Silencing BST2 was associated with a statistically significant reduction in the viability of T47D (p value < 0.05) but had no impact its proliferation (p value = 0.6). Lastly, the downstream effects of BST2 silencing …
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Cytochrome P450 mRNA profile in human breast cancer cell lines.
… the study of regulatory mechanisms of CYP4Z1, T47D and ZR-75-1 BCCs were used in order to determine the appropriate nuclear receptors - i.e. progesterone receptor, glucocorticoid receptor or peroxisome proliferator-activated receptor alpha. The findings suggest that there may be an alternative …
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The regulation of the T-box transcription factor TBX3 in Luminal A breast cancer
… c-Myc is ectopically overexpressed in MCF-7 and T47D breast cancer cells in the presence of Actinomycin D, an inhibitor of de novo transcription, the ability of c-Myc to activate TBX3 mRNA levels is abolished. Together, these results suggest that c-Myc transcriptionally activates TBX3 in breast …
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The anticipatory unfolded protein response and estrogen receptor mutations in breast cancer
… technology to replace wild-type ERα in T47D, human breast cancer cells, with the most common mutations, ERαY537S and ERαD538G. The mutant cells exhibit partially estrogen-independent and antiestrogen resistant gene expression and cell proliferation. A novel …
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Identification of tumour-associated gycoproteins recognised by the lectin from Helix pomatia in breast cancer
… primary cancer (BT474) to metastatic cancer (T47D, MCF-7). HPA binding was assessed using confocal microscopy. Membrane proteins were extracted by differential centrifugation and the proteins were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with Western …
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Comparative efficacy of fractionated Cape cobra (Naja nivea) venom on breast and cervical cancer cells
… testing. These were evaluated on breast (MCF7, T47D, MDA-MB-231) and cervical (CaSki, SiHa, HeLa) cancer cell lines, alongside non-malignant fibroblast (FG0) and retinal pigment epithelial (ARPE-19) cells. Short-term cytotoxicity was assessed by MTT assay and IC50 values calculated. Based on …
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The Interaction of Equol and Genistein on Estrogen Dependent Tumors and Bones in Ovariectomized Athymic Nude Mice
… to evaluate the effect of equol alone on the T47D human breast cancer cells and bone metabolism. At the physiological concentration levels, 100 nM equol stimulated the T47D cell proliferation similarly as 0.1 nM 17-beta-estradiol in vitro. However, 250 ppm and 750 ppm dietary equol, which gave …
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Crosstalk of TTC5 cofactor and the estrogen receptor in breast cancer
… with estrogen receptor alpha (ERa) inMCF-7 and T47D breast cancer cells, and that ER transcriptional activity on an estrogenresponse element was regulated by different TPR and LXXLL motifs of TTC5 in a cellspecificmanner. Partial knockdown of TTC5 via siRNA led to a decrease of proliferationin …
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Small RNAs Regulate Transcription by Interacting with Noncoding RNA Transcripts
… levels between 10 and 1000 fold lower than PR in T47D and MCF7 breast cancer cells. I have cloned three transcripts overlapping the promoter of PR from two cell lines – T47D and MCF7, each with unique splicing and transcription start sites. All of these transcripts initiate within the protein …
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Differential membrane-type matrix metalloproteinase expression in phenotypically defined breast cancer cell lines: Comparison of MT-MMP expression in environmentally-challenged 2D monolayer cultures and 3D multicellular tumour spheroids
… using different breast cancer cell lines (MCF-7, T47D, MDA-MB-468 and MDA-MB-231) was performed. Unexpected detection of MT1-MMP in MCF-7 MCTS warranted further investigation. MT1-MMP expression in different micro-environmental conditions, including hypoxia and nutrient deprivation (serum-free …
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Small molecule inhibitors: novel probes of progesterone receptor action
… and endogenous alkaline phosphatase activity in T47D cells with IC50s in the low micromolar range. They inhibit PR with limited cross-reactivity for inhibiting other steroid receptors, estrogen receptor (ER), glucocorticoid receptor (GR), and androgen receptor (AR). Our in vitro studies show that …
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The role of human DEAD-box protein DDX3X in the activation and function of Estrogen Receptor-alpha
… effect of DDX3 knockdown on the proteome of the T47D breast cancer cell line grown under estrogen-replete and -deplete conditions. DDX3 knockdown lead to decreased expression of three chaperones involved in regulating ERα activity, Hsp90, Hsp70 and FKBP52. DDX3 was previously also shown to …
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A study of the regulation of oestrogen receptor alpha by tissue factor in breast cancer cells
… mechanisms. Incubation of the MCF-7 and T47D breast cancer cell lines with exogenous TF resulted in decreases in the expression of ERa mRNA and protein by a mechanism requiring the interaction of TF with β1 integrin leading to the activation of the ERK1/2 pathway, but was independent of …
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Rekombinante Antikörper gegen tumorassoziiertes MUC1
… natives, von Zellen der Mammakarzinom-Zelllinie T47D präsentiertes MUC1 bindet. Die mit Hilfe der Oberflächen-Plasmon-Resonanz ermittelten Affinitäten des generierten scFv-Fragments zu tumorassoziiertem MUC1 und synthetischem MUC1-Glykopeptid betragen 2,75 x 10-7 M bzw. 2,28 x 10-7 M. Das …
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