Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 62 for “"T3SS"”.
-
Mechanisms of signal integration: Regulation of the SPI1 T3SS
… cells using a type 3 secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). The system is controlled by three AraC-like regulators HilD, HilC, and RtsA, which form a complex feed forward loop to activate expression of hilA, encoding the main transcriptional …
-
Molecular Interactions of Type III Secretion System Transcriptional Regulators in Pseudomonas aeruginosa: ExsA and ExsD
… infections. The type III secretion system (T3SS) aids acute P. aeruginosa infections by injecting potent cytotoxins (effectors) into host cells to suppress the host's innate immune response. Expression of all T3SS-related genes is strictly dependent upon the transcription factor ExsA. …
-
Structural study of ExsA, the regulator of Type III Secretion System of Pseudomonas aeruginosa
The Type III secretion system (T3SS) of Pseudomonas aeruginosa uses a needle-like protein apparatus to detect eukaryotic host cells and translocate effectors directly into the host cell. The effectors are also known as cytotoxins, which cause disruption of a series of signaling events in the host …
-
Identification and characterization of type III secretion inhibitors in Erwinia amylovora, the causal agent of fire blight of apple and pear
… of fire blight. The type III secretion system (T3SS) and exopolysaccharide (EPS) amylovoran are two major yet separate virulence factors in E. amylovora. Current fire blight management mainly relies on application of copper compounds and antibiotics. However, development of streptomycin …
-
Structural and Functional Characterization of SPOA Domains in Salmonella Typhimurium Type III Secretion Systems
… therapeutic design. Type III secretion systems (T3SS) are employed by some gram-negative human pathogens to manipulate the host environment. One T3SS subtype, known as the "injectisome," delivers virulence factors directly into host cells. The other T3SS subtype secretes the the polymeric …
-
Role of new virulence mechanisms/factors (type 3 secretion system and TOX-R regulated lipoprotein [TAGA]) in the pathogenesis of the emerging human pathogen Aeromonas hydrophila
… and characterized a type 3 secretion system (T3SS) in A. hydrophila SSU. By marker-exchange mutagenesis of the aopB gene, a crucial gene involved in the formation of the translocon apparatus, the functionality of the T3SS was elucidated, both in in vitro and in vivo models. Further, the …
-
THE BIPC MINOR TRANSLOCASE MEMBRANE PROTEIN OF THE BURKHOLDERIA TRANSLOCON
… bacteria utilize type III secretion system (T3SS) as a tool in their arsenal in the pursuit of host invasion. The T3SS works to the advantage of the pathogen and acts like a syringe, cleverly bypassing any of the host immune responses, which injects effector proteins (virulence factors) into …
-
Posttranscriptional and posttranslational regulation of virulence in erwinia amylovora
… factors, the type III secretion system (T3SS) and the exopolysaccharide (EPS) amylovoran, are strictly required for its pathogenicity. Our previous studies have determined the role of several transcription factors in the regulation of E. amylovora virulence; however, molecular mechanisms …
-
Tagging of the type three secretion system basal apparatus of Enterohaemorrhagic Escherichia coli 0157:H7
… O157:H7 possesses a type III secretion system (T3SS). The T3SS translocates effector proteins into host cells during infection and consists of a basal apparatus that spans the inner and outer membranes and a needle and filament complex. The basal apparatus is composed of -20 proteins that are …
-
Role of Salmonella enterica subspecies enterica serovar Enteritidis pathogenicity island-2 in chickens
… encode a specialized type III secretion system (T3SS) that enables Salmonella to manipulate host cells at various stages of the invasion/infection process. The SPI-2 T3SS has been identified as vital for survival and replication of S. Typhimurium and S. Enteritidis in mouse macrophages, as well …
-
Intracellular triggering of inflammation by the extracellular bacterium Pseudomonas aeruginosa
… infection is the type III secretion system (T3SS). This system is found exclusively in Gram-negative bacteria and it forms a conduit between the bacteria and the host cell through which effector molecules can be translocated. These effectors alter the function of the host cell to promote …
-
Molecular mechanisms underlying regulation of type III secretion system upstream of the master regulator HrpL in Erwinia amylovora
… pathogenicity (hrp)-type III secretion system (T3SS). It has been previously demonstrated that HrpL, an ECF sigma factor, is the master regulator of hrp-T3SS. Recently, it is reported that expression of hrpL is under the control of sigma 54 complex, including σ54 (RpoN), its modulation protein …
-
Protein glycosylation by NleB and the secretion of therapeutic nanobody fusions
… hosts by using a type III secretion system (T3SS) to secrete effectors into host cells. NleB and SseK are two E. coli and Salmonella effector orthologs that are unusual glycosyltransferases. They glycosylate host protein substrates on arginine residues with N-acetyl glucosamine (GlcNAc) to …
-
Untersuchungen zur Proteinsekretion in Bradyrhizobium japonicum unter besonderer Berücksichtigung des Typ III- Sekretionssystems und Charakterisierung der „metal ion-inducible autocleavage“ Effektordomäne
… die Untersuchung des Typ III-Sekretionssystem (T3SS) bei Bradyrhizobium japonicum USDA110. Im ersten Teil der Arbeit wird die Regulation der Gene des Typ III-Sekretionssystems in B. japonicum beschrieben. Dabei wurde die tts-Box, als neuartige Promotorsequenz für die Gene des T3SS und …
-
Regulation of the Salmonella Pathogenicity Island 1 Type Three Secretion System
… is mediated by a Type Three Secretion System (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). The SPI1 TTSS injects effector proteins into the cytosol of host cells where they promote actin rearrangement and engulfment of the bacteria. HilC, HilD, and RtsA activate expression of SPI1 …
-
The Role of Periplasmic Disulfide Bond Status in the Regulation of the Salmonella SPI1 Type Three Secretion System
… Island I (SPI1) type III secretion system (T3SS) to induce inflammatory diarrhea and bacterial uptake into intestinal epithelial cells. The master SPI1 regulatory gene hilA is controlled directly by three AraC-like regulators: HilD, HilC and RtsA. A periplasmic disulfide bond oxidoreductase …
-
Signaling Specificity in a Campylobacter jejuni Two-Component System to Mediate Proper Flagellar Gene Transcription
… the flagellar type III secretion system (T3SS), the FlgSR two-component regulatory system (TCS), and the FlhF GTPase as requirements to positively regulate expression of σ54-dependent genes that encode flagellar rod and hook proteins. Our laboratory previously proposed that signal …
-
Elucidating three novel mechanisms of Pseudomonas syringae pathogenicity
… proteins through a Type Three Secretion System (T3SS) to suppress the immune response elicited by their MAMPs. However, secretion of effectors can also trigger a strong plant immune response if the plant harbors resistance proteins capable of recognizing the secreted effectors. Successful …
-
An analysis of selected secretion systems of Pseudomonas species
… the roles of the Type III Secretion System (T3SS) and the Type VI Secretion System (T6SS) systems of Pseudomonas, a widespread genus including clinical pathogens and biocontrol strains. Bioinformatic analysis of T6SS phylogeny and associated gene content within Pseudomonas identified several …
-
Towards Understanding the Genetic Basis of Virulence in Pseudomonas aeruginosa
… the variation in type III secretion system (T3SS) expression in P. aeruginosa isolates obtained from the lungs of CF patients to investigate the prevalence of spontaneous activation of the T3SS. To do this, I compared approximately 1200 isolates from a cohort of nine CF patients collected …
Page 1 of 4