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Showing 1 to 20 of 202 for “"T cell receptor"”.
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Characterization of a single-chain anti-T cell receptor antibody and a single-chain T cell receptor
The T cell receptor (TCR) is a cell surface protein that specifically binds to a foreign antigen on the surface of another cell. The TCR is made up of two disulfide-linked chains (called $\alpha$ and $\beta$) each containing a variable and a constant domain. The tertiary structure and binding …
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Directed Evolution of T Cell Receptor Antagonists
… shown to completely and specifically inhibit T cell activity in vitro.
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Mechanisms of generating T cell receptor diversity
T cells are able to respond to foreign antigens by means of a diverse repertoire of antigen-specific receptors (T cell receptors or TCRs). There are two classes of TCRs ($\alpha\beta$ and $\gamma\delta$) that are found on mutually exclusive populations of T cells. Diversity in each receptor subunit …
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Molecular genetics of the T cell receptor alpha chain
… and nucleotide sequence analysis to examine T cell receptor (TCR) alpha chain diversity in fetal and adult thymi. Transcripts were amplified using various V$\alpha$ and C$\alpha$ specific primers and the cloned PCR products were hybridized with J$\alpha$ specific oligonucleotide probes in order …
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Characterization of a T cell receptor and its ligands
Cytotoxic T lymphocytes (CTL) recognize and lyse cells presenting viral or tumor-associated antigens associated with class I major histocompatibility complex proteins. Recognition occurs between the T cell receptor (TCR) and a complex of an antigenic peptide/major histocompatibility complex (MHC) …
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High-throughput tools for decoding T cell receptor specificity
T cells play a central role in adaptive immunity by recognizing specific antigens through their T cell receptors (TCRs). These receptors bind to peptides presented by major histocompatibility complex (pMHC) proteins, driving immune responses in cancer, infection, and autoimmunity. Understanding how …
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Regulation of the Alpha and Delta T Cell Receptor Repertoires
… space are assembled to generate unique antigen receptors by a cut-and-paste reaction in somatic cells. The mechanisms that regulate V(D)J recombination remain unclear.In T cells, two receptor classes are possible. These TCRs, the TCR and TCR, are assembled in part from genes sharing a single …
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Characterization of a T Cell Receptor: Peptide/MHC Binding Interaction
To begin to examine the 2C TCR residues which provide a significant thermodynamic contribution toward the binding energy of a variety of ligands, including monoclonal antibodies (mAb), superantigens, and peptide/MHC ligands, we have expressed a family of 2C scTCRs which contain alanine point …
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T-cell receptor diversity of various antigen-specific T cells
T cells recognize foreign antigen through a heterodimeric $\alpha\beta$ T-cell receptor (TCR). The TCR ligand is a peptide bound to a membrane-bound protein coded by the major histocompatibility complex (MHC) locus. Like immunoglobulins, the TCR repertoire is diverse because both the $\alpha$ and …
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Statistical physics of T cell receptor development and antigen specificity
… made in understanding pertinent molecular and cellular phenomena, the mechanistic principles that govern many aspects of an immune response are not known. In this thesis, I illustrate how complementary approaches from the physical and life sciences can help confront this challenge. …
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The interferon alpha receptor utilises T-cell receptor-associated proteins for signalling
The interferon alpha receptor (IFNAR) and T-cell receptor (TCR) are expressed upon the T-cell surface. The dimeric Class I interferon receptor is a cytokine receptor that recognises interferons such as IFNα. Interferons (IFNs) are pluripotent, antiviral cytokines that causes antiproliferative …
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Kinetic analysis of Fcγ receptor and T cell receptor interacting with respective ligands
Low affinity Fcg receptor III (FcgRIII, CD16) triggers a variety of cellular events upon binding to the Fc portion of IgG. A real-time flow cytometry method was developed to measure the affinity and kinetics of such low affinity receptor/ligand interactions, which was shown as an easily operated …
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Staphylococcal Enterotoxin B Induced Clonal Anergy in αβ T Cell Receptor Transgenic Mice
… B (SEB) have been widely used to study T cell tolerance. While both clonal deletion and apparent anergy have been described in response to superantigens, it has not been clear whether true anergy occurs, or whether the most reactive T cells are selectively deleted, leaving less reactive …
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The Role of T Cell Receptor: Peptide MHC Affinity in T Cell Activation
Engineering of TCRs requires stabilization of the receptor prior to engineering for higher affinity. Chapter 4 details the engineering of a high affinity TCR in its native state, without the need for stabilization, using a T cell display system. T cells bearing the newly isolated TCR bound pepMHC …
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Mismatch Repair Deficient Neoantigen and Associated Circulating T-Cell Receptor Repertoires in Lynch Syndrome
… development effectively. Furthermore, the T-cell receptor (TCR) landscape of LS patients, particularly those that recognize these neoags, has never been investigated. Thus, there is an excellent opportunity for exploring immune-based preventive strategies and gaining insights into the …
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Engineering the T Cell Receptor to Protect Host Cells in Superantigen-Induced Septic Shock
… these proteins target a subset of white blood cells, namely T cells, which are responsible for the cell-mediated responses of the immune system. SAgs stimulate T cell populations by crosslinking a T cell receptor (TCR) with a class II major histocompatability complex (pMHC) product on the host …
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Binding, Thermodynamic and Structural Studies of High-Affinity T Cell Receptor-Peptide MHC Interactions
Finally, in chapter 5, fluorescence spectroscopy was used in stability and binding studies of scTCRs. Urea denaturation analysis was used to show that scTCRs engineered in the yeast display system have similar stabilities as single chain antibody Fvs. The yeast display system was successful in …
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T cell receptor- and calcium-induced Gli signalling in CD8+ T cell effector function
… T lymphocytes (CTLs) are potent effector cells of the immune system, capable of directly lysing neoplastic and infected cells. The Hedgehog (Hh) signalling pathway - a critical regulator of embryonic development and adult tissue maintenance - is induced in CTLs following TCR stimulation …
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Quantitative dynamics of T-cell receptor repertoires and their use for cancer patient stratification
T-cells in the adaptive immune system can recognise a vast space of potentially foreign antigens in a very specific way through their T-cell Receptors (TCR). As the genetic sequence encoding for the TCR is the result of somatic recombination, the TCR sequence varies from T-cell to T-cell, making it …
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