Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"T cell priming"”.
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FAMIN in dendritic cells biochemically restrains T cell priming
… against influenza A infection, with dendritic cell (DC)- dependent excessive activation of CD8<sup>+</sup> T cell responses in their FAMIN-deficient counterparts. Loss of FAMIN activity in DCs was shown to result in increased priming of both CD4<sup>+</sup> and CD8<sup>+</sup> T cells both …
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Cellular and Molecular Aspects Of Naïve T cell Priming for Adoptive Immunotherapy
… in the advancement of immunotherapy. Adoptive cell therapy (ACT) is a modality that aims to generate a robust tumour-directed response by the infusion of tumour specific, ex vivo expanded, autologous cells. In this thesis we examine aspects of naïve T cell culture with the aim of improving …
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MHC Class I Tyrosine Phosphorylation Site Y320 Augments CD8+ T Cell Priming, Effector Function, and Memory Response
… and antigen cross-presentation by dendritic cells (DCs). In this study, we investigated whether there are additional, post-presentation dependencies on Y320 for T-cell priming. We engineered both human- and mouse-derived antigen-presenting cells (APCs) to express either wild-type MHC-I or …
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Role of Multiple Toll-Like Receptor Activation in Regulating Cytotoxic T Cell Priming to Lympocytic Choriomeningitis Virus Infections
… receptors (TLR), present on various immune cells such as professional antigen-presenting cells (pAPCs). On recognition of its ligand, these receptors activate pAPCs, which may in turn influence naïve CD8+ T cell activation and affect their abilities to clear viral infection. However, how TLR …
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Environmental stimuli shape adaptive immunity by enhancing T helper cell differentiation and the germinal centre response
Immune cells have evolved to respond to external danger signals and to incorporate information about environmental cues to adapt their differentiation and effector function. Because of this, it is important that immune cells are studied in the context of their surroundings. In this thesis, I …
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Atr-Mediated Cd47 and Pd-L1 Upregulation Restricts Radiotherapy-Induced Immune Priming and Abscopal Responses In Colorectal Cancer
… tumor-associated antigen (TAA)-expressing CRC cells systemically. However, incidences of abscopal tumor remission are extremely rare, and the post-irradiation immune escape mechanisms in CRC remain elusive. We report that CRC cells utilize a common DNA repair signaling pathway — ATR/Chk1/STAT3 …
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Dendritic Cells Mediate the Dual Effects of IFN alpha/beta in HCV Immunity
… individuals and correlates with a strong T cell response. Furthermore, treatment with interferon (IFN)α achieves sustained virologic response in approximately 50% of chronically infected individuals. Conventional dendritic cells (cDCs), as the most potent antigen presenting cells, and …
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Layer-by-layer coated microneedles for cancer immunotherapy
… strategies to boost antigen presentation and T cell priming in order to increase the fraction of patients responding to immunotherapy remains an urgent need. For the delivery platform, we applied microneedle (MN) skin patches to transdermally deliver vaccines to activate the potent epidermal …
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Dendritic cell dysfunction restrains cytotoxic T cell responses against cancer
… are often characterized by the presence of a T cell infiltrate in tumors. However, T cell infiltration does not always correspond to ICB efficacy. Tumor-reactive T cells can acquire persistent dysfunctional states, which are resistant to ICB reinvigoration. Increasing evidence suggests that T …
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Integrated, single-cell analysis of transcriptional phenotype and clonotypic identity
Single-cell RNA sequencing (scRNA-seq) currently affords the ability to resolve whole transcriptomes of single cells with substantial throughput and has revolutionized studies of gene expression. Recent technical advances have enabled the matching of T cell receptor (TCR) and B cell receptor (BCR) …
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Dendritic Cells Suppress Pathogen-Induced Inflammasome Activation to Prime Naïve T Cells
Activation of inflammasome leads to pyroptotic cell death thereby eliminating the replicative niche of virulent pathogens, a process integral to innate immunity. While inflammasome-associated cytokines such as IL-1β and IL-18 have an established role in T cell function, whether inflammasome …
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A transcriptomic approach for studying the activation of dendritic cells in response to mycobacterial infections
… aid in the design of a better vaccine. Dendritic cells are potent antigen presenting cells that play a key role in priming naïve T cells. Effective T cell priming is necessary for a successful protective immune response in the host against the pathogen. I have been investigating the interaction of …
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Targeting Myeloid Protein Kinase C Signaling to Overcome Immune Suppression and Improve Immunotherapy in Cancer
Checkpoint immunotherapy unleashes T cell antitumor potential which has revolutionized cancer treatment showing unprecedented long-term responses. However, most patients do not respond to immunotherapy which often correlates with a dysfunctional or immunosuppressive myeloid compartment. …
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Targeting of specific developmental pathways to understand dendritic cell heterogeneity and function
Dendritic cells (DC) are cells of the immune system that are specialized in sensing and responding to pathogens. They coordinate innate and adaptive immune responses to different types of pathogens through the release of inflammatory cytokines, secretion of interferon, and presentation of antigen …
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Engineering immunity : enhancing T Cell vaccines and combination immunotherapies for the treatment of cancer
… this thesis, we describe methods for enhancing T cell priming against tumor antigens via covalent modification of molecular vaccines to enhance lymphatic drainage, serum stability, or cytosolic access to improve presentation on MHC class I. In Chapter 4, we demonstrate a combination immunotherapy …
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A materials-based approach for localized delivery of cancer immunotherapy
… phosphoserine (pSer) residues, through in-cell phosphorylation during recombinant expression. Cytokines with pSer tags bind tightly to the common vaccine adjuvant aluminum hydroxide (alum) via ligand exchange. Intratumoral injection of pSer-cytokine-loaded alum led to prolonged retention of …
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Innate Interleukin-17 and Interleukin-22 play important roles in viral hepatitis
Intrahepatic cell-derived IL-17 and IL-22 are important for T cell priming and hepatoprotection in viral hepatitis, respectively; however, the source and regulation of these cytokines in the liver microenvironment are not well defined. Here, we present evidence for a significant expansion of IL-17- …
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Novel Mechanisms of Antigen Processing That Enhance Bcg Vaccine Efficacy
… fusion is necessary for peptide production and T cell priming leading to protective T<sub>H</sub>1 immunity, we hypothesized that vaccine efficacy is reduced and occurs perhaps due to non-lysosome dependent mechanisms. We therefore proposed an in depth analysis of phagosome environment, and its …
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The effects of topical calcipotriol treatment on immune responses to vaccination
… capable of generating regulatory T cells (Tregs) and contributing to immune tolerance. Additionally, vitamin D has been shown to promote mucosal immunity when used as a vaccine adjuvant. We show here that pretreatment of an area of skin with the synthetic vitamin D analog …
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Local or Systemic Antibody-Targeted Sting Activation Drives Pro- Inflammatory Stromal Metabolic Changes and Downregulates c-MYC Oncoprotein
… of the myeloid stroma, thus enhancing T cell priming and infiltration. However, systemic delivery of these small molecule STING agonists has been limited by their rapid diffusion and clearance, as well as the associated risks of systemic toxicity, confining their application primarily to …
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