Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"Synthetic peptides"”.
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Regulatory issues concerning the preclinical testing of synthetic peptides
… which preclinical studies are to be conducted. Synthetic peptides present a unique scenario in which a case-by-case approach is needed for the conduct of preclinical studies. For peptides containing components that are already tested for genotoxicity, it is unnecessary to reevaluate them. If …
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Antibody production with synthetic peptides against human Coronavirus-NL63 ORF3 protein
… and antigenic epitopes were used to generate peptides of ORF3 protein. Thereafter, the ORF3 protein was characterized using various molecular techniques across the bacterial and mammalian expression systems. A CoV-NL63 full-length ORF3 protein (Protein Identity Number: ABE97138.1) was utilized …
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Synthetic peptides derived from decorin as building blocks for biomaterials based on supramolecular interactions
… of a new molecular building block, based on synthetic peptides derived from decorin, is presented. These peptides represent a promising basis for the design of polymer-based biomaterials that mimic the ECM on a molecular level and exploit specific biological recognition for technical …
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Synthesis Of Non-Natural Fmoc-Protected Amino Acids To Provide Novel Fluorescent Anion Probes And Their Incorporation Into Synthetic Peptides
… eventual incorporation into membrane-spanning peptides. Amino acids 2.1 and 2.2 were successfully synthesized from commercially available Fmoc-Glu-OtBu and Fmoc-Asp-OtBu through the conversion of an acid azide into an isocyanate via a Curtis Rearrangement. An N-substituted-4-amino-naphthalimide …
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Development and Application of Chemoenzymatic Tools to Investigate Site-Specific Protein ADP-Ribosylation
… and poly-ADP-ribosylation at specific sites on synthetic peptides and full-length proteins. Historically, chemical and topological complexities associated with ADP-ribosylation have stymied progress in this area. To overcome this, I have identified enzymes capable of: (i) installing ADP-ribose …
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A Structural and Functional Analysis of the Human Angiotensin II Type-2 Receptor
In the third series of experiments we used synthetic peptides derived from the amino acid sequence of the cytoplasmic loops and tail of the AT 2 receptor are used to determine what regions are important to G-protein activation. These studies will map the G-protein coupling regions of the human AT2 …
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Novel Endogenous Antimicrobial Peptides
Antimicrobial peptides serve as a first line of defence against invading microorganisms and are an essential part of our fast innate immune system. They are ancient molecules found in all classes of life. Antimicrobial peptides rapidly kill a broad spectrum of microbes and are immunomodulatory, …
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Elucidation of antigenic epitopes on the rubella virus E1 glycoprotein
… persistent infection are discussed. Twenty three synthetic peptides spanning the entire El sequence were screened with human peripheral blood lymphocytes and the corresponding sera to examine the distribution of antigenic domains. El glycoprotein was also the target of viral neutralizing (VN) and …
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Antigenic properties of snake venom cardiotoxins
… applying the knowledge obtained in the design of synthetic vaccines. The conformation and surface properties of this toxin differs from that of proteins of which the immunological properties are known, so that this information cannot be directly applied.Parallel to the investigation of the …
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NOVEL RNA-BASED CANCER VACCINE FOR AML IMMUNOTHERAPY
… Screen for immunogenic neoepitopes using 102 synthetic peptides was performed in ELISpot assays. Our final 10 candidates were cloned into a vaccine construct. mRNA cancer vaccines were synthesized using our optimized laboratory-scale synthesis pipeline. Using manganese lipid nanoparticles as a …
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Peptide Antisera Generation against Three <em>Chlamydia trachomatis</em> Hsp60 Homologues to Examine Expression of each Hsp60 during Iron Restrictive Growth.
… amino acid sequences were evaluated for peptides unique to each chsp60. Synthetic peptides were used to generate antisera; the resultant sera were purified by affinity chromatography and adsorbed to reduce cross-reactivity and increase monospecificity. Antisera were evaluated against each …
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CONTROLLING BIOFILM AND PERSISTER CELLS BY TARGETING CELL MEMBRANES
… the antimicrobial activities of newly designed synthetic peptides on Escherichia coli and Pseudomonas aeruginosa strains including regular planktonic cells and those in biofilms and at the persister stages. Our results revealed that 2D-24, an RW-rich dendrimeric peptide, can kill planktonic …
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Analyzing Functional Interactions of Designed Peptides by NMR Spectroscopy
… Here, we report the analysis of two groups of synthetic peptides designed for two applications – broad bactericidal action and inhibition of protein-protein interactions in human cells. Novel amphiphilic peptides designed for antibacterial application incorporated arginine as cationic amino …
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Identification and activity assessment of a bioactive peptide QUB-2642 from the skin secretion of The American Bullfrog (Rana catesbeiana)
… in microbial pathogens has made antimicrobial peptides a very attractive option for new-generation antibiotics. Amongst antimicrobial peptides widespread in different classes of life, those derived from amphibians have been receiving special research attention since the last century. Secreted …
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Production of Monoclonal Antibodies for Toxoplasma gondii Lactate Dehydrogenase
… 6-8 week old BALB/c mice were immunized with synthetic peptides of TgLDH, splenocytes were harvested. To get the monoclonal antibodies (McAbs), splenocytes were fused with myeloma cells using PEG by a cell fusion technique. Hybridoma cells were selected by ELISA and subcloned through a …
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In vitro effects of different innate-immune stimulants on Chinook salmon embryo cell line (CHSE-214): transcriptional and translational studies
… cathelicidin by immunization of rabbit with synthetic peptides derived from cathelicidin. Specific cathelicidin antibodies were not obtained in this experiment. The findings might contribute to the development of alternatives to the classical prophylactic methods, antibiotics and vaccination …
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Chemosensing strategies : utilizing the novel sulfonamidohydroxyquinoline amino acid Sox
… this biologically-important metal ion. Nineteen synthetic peptides incorporating Sox exhibit a range of affinities for Zn²⁺ through variation of the type and number of Zn²⁺ ligands, ligand arrangement and the [beta]-turn sequence that acts as a preorganization element between the ligands. The …
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NMR studies of dimeric and polymeric lactams.
… a powerful β-turn- inducer when incorporated in synthetic peptides. The cis- lactam was found, however, to have a very similar conformation in DMSO solution, without hydrogen bonding to stabilize the turn. No evidence was found for the β-turn in the dimers and polymers using 2-dimensional NMR …
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Collagen-mimetic peptides for diagnosis and analysis
… is a technology known as collagen-mimetic peptides (CMPs), which are synthetic peptides that mimic the natural structure of collagen. These peptides can be applied to study collagen structure and function, from its macromolecular architecture in tissues to the significance of molecular …
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Development of Polymer Monoliths for the Analysis of Peptides and Proteins
… novel polymer monoliths for the analysis of peptides and proteins were synthesized using polyethylene glycol diacrylate (PEGDA) as crosslinker. Photo-initiated copolymerization of polyethylene glycol methyl ether acrylate and PEGDA yielded an inert monolith that could be used for size …
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