Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 6 of 6 for “"Synthetic Promoters"”.
-
Improving Thermodynamic Models of Transcription by Combining ChIP and Expression Measurements of Synthetic Promoters
… of a reporter gene driven by many similar promoters . Models parameterized in this fashion do better at explaining the sequence to expression relationship, but fail to distinguish between multiple biological mechanisms that give rise to equivalent expression results in the synthetic …
-
Genetic and Computational Tools for Engineering Complex Prokaryotic Systems
… challenges for engineering efficient biosynthetic pathways. While CRISPR-Cas technologies have revolutionized genome editing in eukaryotes, the development of programmable, multi-layer transcriptional control in bacteria is still emerging. This work outlines our efforts to expand the …
-
Architecture of Escherichia coli promoters that respond to reactive nitrogen species
… activation by NarL was then studied using semi-synthetic promoters. The hcp gene was found to be positively regulated by FNR and negatively regulated by NsrR. Contrary to previous reports, NarL and NarP were found to have little direct effect on expression of hcp, however, an indirect effect of …
-
Tools for engineering multicellular systems through cell sorting and cell state detection
… able to play the role it needs within our body. Synthetic biology, an emerging field in biology, seeks to expand on this blueprint and create cells with novel functions. The aim of this thesis is to provide methods that expands our ability to engineer and control multicellular systems by …
-
The interaction of promoter chromatin architecture with the cell cycle regulates transcription activation kinetics
… To study the activation kinetics of individual promoters, we develop a method to infer real-time transcription rates from protein expression in single Saccharomyces cerevisiae cells using time-lapse fluorescence microscopy. Comparison between the instantaneous transcription rate and cell-cycle …
-
Application of Single-Molecule Analytical Methods for the Characterization of Multimeric Genetic Elements in Yarrowia lipolytica DSM 3286
… have the potential to be used to design new synthetic promoters or to adapt homologous transcription regulation systems. <br /> The results of this research show that ultra-long sequencing reads cannot only improve the continuity and completeness of genome assemblies but also provide new …