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Showing 1 to 4 of 4 for “"Synthetic Genomics"”.
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Engineering genetic isolation in Escherichia coli and developing synthetic genomics tools to efficiently assemble megabase-scale genomes
… biological systems at various scales. Short synthetic DNA oligos used as primers for polymerase chain reaction (PCR) enable the specific amplification of chosen DNA sequence and synthetic genes are critical for the design and directed evolution of proteins. The synthesis of entire genomes …
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Understanding Genomes Through Engineered Structural Variation
Sequencing of the human genome has provided us with a detailed map of its content. While enormous progress has been made towards understanding the 1% of the human genome that is protein coding, we are still mostly in the dark about the function and relevance of the remaining 99%. Progress has been …
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Engineering Escherichia coli as a general genome synthesis platform
… For the synthesis of the E. coli genome, small synthetic DNA (1–10 kb) pieces are assembled to bacterial artificial chromosomes (BACs, 50–100 kb), which are then used to replace wildtype sequence in the E. coli genome. Conjugation coupled with programmed excision for enhanced recombination …
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Rewriting the genome of Escherichia coli
… synonymous replacements is challenging. Second, synthetic DNA pieces need to be assembled into fragments of increasing size, and ultimately implemented inside a target host. Here, recently reported strategies for genome engineering in E. coli (REXER and GENESIS) are extended and used to create a …