Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Synthetic Cell"”.
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Expanding the synthetic cell toolkit: programmable fusion for complex genetic circuits and a synthetic cell cycle
Synthetic biology as a scientific discipline is relatively new; however, major strides have been taken to develop current in vitro technologies into platforms that can imitate life. Currently, synthetic cell technologies often address only one feature of life (cell division, protein expression, DNA …
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Homeostatic mechanics for a synthetic cell: predictions on substrate anisotropies and cell-cell interactions
… one or more biological processes, which in cells are known to be highly coupled. This added complexity limits the control over the biocompatibility and biofunctionality of the designed tissues. Amongst the many degrees of freedom in question, substrate topological and mechanical properties, …
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Tools for engineering multicellular systems through cell sorting and cell state detection
… human genome, the genetic blueprint that every cell in our body follows, encodes approximately 20,000 genes. Through complex regulation of these genes, each cell is able to play the role it needs within our body. Synthetic biology, an emerging field in biology, seeks to expand on this blueprint …
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Structural and functional complexity in synthetic cells from amphiphilic DNA nanostars
… to localise and segregate function in biological cells. The most fundamental compartment is the cell itself, being physically delimited from its surroundings *via* the phospholipid cell membrane. Sub-compartments within the cell, known as organelles, may also be membrane-bound or membrane-less. …
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A biological simulator using a stochastic approach for synthetic biology
Synthetic Biology is a new engineering discipline created by the development of genetic engineering technology. Part of a new engineering discipline is to create new tools to build an integrated engineering environment. In this thesis, I designed and implemented a biological system simulator that …
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Building integrated cell-based microsystems : fabrication methodologies, metrology tools and impact on cellular physiology
… microsystems, and (3) the construction of live-cell stress reporters for the characterization of electric-field effects on cell physiology in microsystems. Materials for biological microsystems have been dominated by the elastomer polydimethylsiloxane (PDMS) and the photopolymer SU-8 which form …
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Towards The Engineering Of A Synthetic Minimal Cell
Cell-free expression (CFE) systems are one of the main platforms for building a synthetic cell. A major drawback is the orthogonality of cell-free systems across species. To generate a CFE system compatible with recently established minimal cell constructs, we attempted to optimize a Mycoplasma …
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A Leap of Fate: Fluid identity, functional modules, and flexible strategies for transcription factor-based reprogramming
… – Norton Juster, The Phantom Tollbooth Direct cell reprogramming is a technique used to convert a cell from one type to another, without a stable intermediate. This is commonly achieved through transcription factor (TF) over-expression in the source cell type, with TF selection based primarily …
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Droplet microfluidics for the study of multiphase fluid dynamics and the development of synthetic cells
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2025-12-01
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Metabolic and genetic factors guiding hematopoietic cell fate
… development requires precise control over cellular identity, and a wide range of diseases is characterized by altered differentiation states. Understanding the environmental factors that interplay with gene expression networks to regulate cell state holds immense potential for both basic …
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Cross-Modality Profiling of High-Content Microscopy Images with Deep Learning
… in high-content phenotypic screening of cells treated with compounds or genetic perturbations produces a large amount of high-dimensional data. One example which we focus on in this thesis is Cell Painting, a standardized pipeline used to capture rich cell morphology. Typically, …
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Molecular recognition, in- and out- of equilibrium, in the actions of bacterial multidrug efflux nanomachines
… pathogens. The AcrAB-TolC pump spans the cell envelope and is powered by proton gradients across the inner membrane to energise the active efflux of an array of chemically unrelated compounds. How such diverse compounds are recognised, while critical metabolites and other molecules needed …