Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Syndecan"”.
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The Syndecan-1 Paradox in HSV-1 Infection and Pathogenesis
… and the resolution of inflammatory responses. Syndecan-1 (SDC1), a major heparan sulfate chain-bearing proteoglycan, traditionally characterized as a primary attachment receptor that facilitates HSV-1 binding and entry through its heparan sulfate chains. However, its broader functional …
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Syndecan-4 regulates cell-surface trafficking and biological activity of transglutaminase-2
Transglutaminase-2 (TG2) is a Ca2+-dependent crosslinking enzyme involved in the post-translational modification of proteins via the formation of Nε(γ-glutamyl)lysine isodipeptides. TG2 is externalised in the extracellular matrix (ECM) through an unconventional and not fully understood pathway. …
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Mechanical Strain-Mediated Syndecan Regulation and Its Effects on Adhesion of Vascular Smooth Muscle Cells
… extracellular environment via regulation of the syndecan class of cell adhesion molecules dictates the response of the vascular wall to local injury. The effect of in vitro syndecan-4 regulation on SMC adhesion was investigated through the use of a glass microsphere centrifugation assay, and an …
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A Novel Syndecan-1 Targeted Therapeutic Antibody Inhibits Macropinocytosis and Induces Anti-Tumor Immunity in Pancreatic Cancer
… for this disease. Our previous work identified Syndecan-1 (SDC1), a highly expressed heparan sulfate proteoglycan, as a critical downstream effector protein of KRAS*, which promotes cancer progression by mediating macropinocytosis. Moreover, SDC1 plays an essential role for the acquired …
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The Expression of Syndecan-1 is Related to the Risk of Endometrial Hyperplasia Progressing to Endometrial Carcinoma
… expression of the cell surface proteoglycan, syndecan-1, is found in many malignancies. The current study describes the immunohistochemical study of syndecan-1 expression in normal, hyperplastic, and malignant endometrial tissues for evaluation of application as a parameter of cancer …
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Identifying novel roles for basement membrane-associated proteins in the adult Drosophila intestinal epithelium
… the conserved transmembrane proteoglycan Syndecan as an essential protein for intestinal stem cell maintenance. Syndecan is a basement membrane receptor, with a plethora of other intra- and extracellular binding partners, that is dysregulated in multiple human diseases. I found that …
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The role of the arterial glycocalyx in sphingosine-1- phosphate induced cardioprotection in the isolated heart of the Wistar rat
… the release of the glycocalyx component syndecan-1 in the coronary effluent using enzyme-linked immunosorbent assay (ELISA) and determining relative syndecan-1 staining intensity between groups in immuno-stained wax sections of perfusion-fixed hearts. In addition, the histo-morphology of …
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The importance of the tissue transglutaminase-fibronectin heterocomplex in the RGD-independent cell adhesion and fibronectin matrix deposition
… mouse embryonic fibroblasts (MEFs) (such as syndecan-4, a5, ß1 or ß3 integrins). In addition specific syndecan-2 targetting siRNAs, ß1 integrin and a4ß1 integrin functional blocking antibodies, and a specific targeting peptide against a5ß1 integrin A5-1 were used to investigate the …
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Investigating the integrity of the vasculature and structural relationships of pericytes, astrocytes and the endothelial glycocalyx in an ex vivo hyperglycaemic rat retinal model
… pericytes, astrocytes and the glycocalyx protein syndecan-1 with anti-neuron-glial 2 antigen (NG2), anti-glial fibrillary acidic protein (GFAP), and anti-syndecan-1 antibodies respectively. Retinal blood vessels were detected with FITC-conjugated lycopersicon esculentum (Tomato) lectin. …
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Circulating proteoglycans and glycosaminoglycans during critical illness
… patients circulating levels of the proteoglycans syndecan-1 was increased compared to controls. No difference was detected between patients with different underlying conditions. This indicates that syndecan-1 release is a general consequence of critical illness that probably has limited value as a …
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Understanding the biological barriers to Jurkat Transfection using non-viral peptides
… (RALA) and two kinds of membrane proteins, syndecan-4 & glypican-1. This hypothesis was verified by transfecting 5 breast cancer cell lines with different levels of syndecan-4 and glypican-1. Results from transfections and RT-PCR illustrated the correlations again.<br/><br/>Finally, an …
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The role of A Disintegrin and Metalloproteinase with Thrombospondin Motifs-‐15 (ADAMTS-‐15) in Breast Cancer
… This anti-migratory effect likely involves syndecan-4, since modulation of syndecan-4 expression and signalling attenuated this effect. In contrast to its effects on cell migration, only wildtype ADAMTS-15 exhibited an anti-angiogenic effect in in vitro and ex vivo models of angiogenesis. In …
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Cell penetrating peptide for ocular drug delivery
… specifically binds to cell surface expressed Syndecan-4 receptors that are upregulated in inflammatory and hypoxic injury. Therefore, the overall hypothesis of this thesis is that the fusion peptide Xentry-Gap19 (XG19) is targeted specifically to injured cells in neovascular AMD to restore …
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Glycans in host-pathogen interactions : an integrated biochemical investigation
… (a large glycan- protein conjugate), syndecan-1 and on epithelial tight junctions. The findings presented in this work suggest mechanisms by which sphingomyelinases could enhance bacterial virulence by regulating epithelial cell function. The second part of the thesis investigates the …
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Mechanotransduction Modulates the Effect of Mechanical Forces (Fluid Shear Stress and Cyclic Strain) on Embryonic Stem Cell Differentiation Toward Vascular Endothelial Cells
… the first evidence that HSPG, integrin, and syndecan-4, as mechanosensors, can contribute to cyclic strain-induced regulation of ESC-derived EC.</p> <p>Recent studies by different groups have acknowledged the effect of mechanical forces on ESC modulation. However, the underlying mechanisms …
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Characterisation of novel matrix-binding interactions for latent transforming growth factor-β-binding protein-2 (LTBP-2), with emphasis on heparin and heparan sulphate proteoglycans.
… tissues HSPGs are found on cell surfaces as syndecans and glypicans and in basement membranes as perlecan. Full length human recombinant LTBP-2 (rLTBP-2) was expressed in 293 EBNA cells using a modified pCEP-4 vector and purified by nickel affinity chromatography. Upon validation of the …
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Fluorous assisted one-pot oligosaccharide synthesis and chemical synthesis of homogeneous heparan sulfate proteoglycan
… Hpearan sulfate proteoglycans (HSPGs), including syndecans and glypicans, are ubiquitous components of mammalian cell surface and extracellular matrix and they play important roles in various biological processes such as cancer development, angiogenesis. viral infection and wound repair. They are …
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Interactions of human immunodeficiency virus type 1 with mucosal epithelial surfaces and Candida albicans
… to promote HIV-1 binding, including GalCer and syndecan-1. Oral and vaginal epithelial cell models can capture HIV-1, which subsequently does not appear to integrate into the epithelial genome. Therefore, viral replication is not supported. Notably, HIV-1 captured on the epithelial surface …
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Heparan Sulfate Proteoglycans in Pancreatic Ductal Adenocarcinoma (PDAC)
… a heparan sulfate proteoglycan (HSPG), Syndecan-1 (SDC1), as a key effector for pancreatic ductal adenocarcinoma (PDAC) progression whose surface expression is driven by KRAS*. By leveraging various PDAC models, we found that surface SDC1 expression was initially diminished upon mutant …
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KSHV Susceptibility and Transmission Within Tonsillar Specimens
… targeting of plasma cells is not due to CD138 (Syndecan-1) being used as an attachment factor, and heparan sulfates, in general, do not play an important role in KSHV infection of B cells. Finally, the donor-dependent immunological factors and immune status of individual samples influences the …
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