Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Synaptotagmins"”.
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An Analysis of Synaptotagmins in Caenorhabditis Elegans
… synaptotagmin family in Caenorhabditis elegans. Synaptotagmins are best known as the calcium-sensing proteins for regulated synaptic vesicle exocytosis. I examined the effects of this lesion on a number of sensory neurons and motor neurons, in order to ascertain the uniformity of the phenotype …
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Regulation of SNARE-Mediated Synaptic Vesicle Release by Synaptotagmins and Complexins
… release. Among these proteins are synaptotagmins and complexins, two protein families that directly interact with the SNARE complex, and that are interdependent to each other in regulating SNARE-mediated synaptic vesicle release: complexin clamps neurotransmitter release until …
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Dissection of the Roles of Synaptotagmins in Calcium Dependent Neurotransmitter Release
… caused by the arrival of an action potential. Synaptotagmins (Syts) mediate this calcium-dependent regulation of neurotransmitter release by mechanisms that still remain elusive. Syts have two calcium-binding C2 domains (C2A and C2B) which are crucial for their function. Clarifying how Syt C2 …
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Structure and Function of Proteins Involved in Regulated Secretion: Synaptotagmins and Complexin
… effect of complexin in neurotransmitter release. Synaptotagmins work with Complexins to trigger rapid membrane fusion in response to calcium influx. Synaptotagmin VII is an important protein for the release of glucagon from islets of langerhans. The C2B domain of this protein is nearly 50% …
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Characterization of Synaptotagmin Function In Calcium Dependent Neuronal Exocytosis
… bilden. Die Funktion der Interaktion Synaptotagmins mit dem SNARE-Komplex ist jedoch weitgehend ungeklärt. Complexine bilden eine weitere Familie von Proteinen, die mit dem neuronalen SNARE-Komplex interagieren and an der Regulation der kalziumabhängigen Exozytose beteiligt sind. Das …
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Molecular genetic characterization of the Drosophila synaptotagmin family
… genome, including homologs of mammalian Synaptotagmins 4, 7, 12 and 14. Using immunolocalization and in situ hybridization experiments (Chapter 2), we demonstrate that each isoform has a unique subcellular localization and expression pattern, suggesting only Synaptotagmin 1 functions in …
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Linking Architecture and Function of Endoplasmic Reticulum - Plasma Membrane Contact Sites
… (Tcbs), the yeast orthologs of Extended-Synaptotagmins, localise to cortical ER of high membrane curvature. A high-throughput genetic screen uncovers a new role for Tcbs in the maintenance of PM bilayer lipid asymmetry and redundancies in lipid routes through other cellular MCS, such as …
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Structural and Functional Studies of C2-Domain Proteins Involved In Neurotransmitter Release
… properties of two types of C2-domain proteins, synaptotagmins and RIMs. With NMR spectroscopy and biochemical assays, we demonstrated that synaptotagmin 4 is a Ca2+ sensor in Drosophila but not in rat, in contrast to the prediction based on sequence alignments. X-ray crystallography revealed …
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Exocytosis in Type 2 Diabetes- Functional and genetic studies of hormone secretion
… SNAP25, Munc-18 (Stxbp1) and the family of Synaptotagmins (Syts). The aim of this thesis has been to investigate functional and genetic events that participate in the exocytotic process. First we show that SNAP25 is essential for cAMP-dependent rapid exocytosis in insulin-secreting cells, …
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Molecular Determinants of Synaptic Vesicle Exocytosis and Endocytosis Coupling
Synaptic vesicle recycling is essential for maintaining normal synaptic function. The reuse of vesicles maintains the size of the presynaptic terminal and ensures the availability of synaptic vesicles for subsequent exocytosis. The coupling of exocytosis and endocytosis allows for continued rapid …
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A Comparative Study of Sensory Neuron Synaptic Activity and the Role of Presynaptic Diversity, Specificity, and Regulation in Caenorhabitis Elegans
… In Chapter 5, we investigate the role of synaptotagmins in AWC<sup>ON</sup> and ASH glutamate release using Vglut-pH imaging. We find that AWC<sup>ON</sup> basal release is highly dependent on snt-1, whereas ASH exocytosis is intact in snt-1 null mutants and slightly diminished in …
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Comparative analysis of membrane-associated signatures at mutualistic and pathogenic plant-microbe interfaces
Plants interact with a diverse range of microbes, both above and below ground, spanning a spectrum from mutualistic relationships (where both partners benefit) to parasitic interactions (where the microbe benefits at the plant’s expense). Many interactions between plants and filamentous microbes …
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Dissecting Roles for the Macromolecular Machinery Involved in Neurotransmitter Release
Neurotransmitter release is a tightly regulated process that involves synaptic vesicle docking at presynaptic active zones, priming of the vesicles to a release-ready state, and calcium evoked fusion of the vesicle and plasma membranes. The probability of release is modulated by plastic changes …