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Showing 1 to 10 of 10 for “"Synaptotagmin I"”.
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Structural characterization of synaptotagmin I
Synaptotagmin I is the most abundant Ca+2 binding protein present on synaptic vesicles accounting for 7% of total vesicle protein and is widely accepted as the Ca2+ sensor in fast synchronous neurotransmitter release. The protein is composed of one trans-membrane domain, an unstructured linker …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
… The mechanism of Ca2+ action is still unknown. Synaptotagmin 1, a brain-specific vesicular transmembrane protein, is the Ca2+ sensor in neurons. It has two cytoplasmic C2 domains (C2A and C2B) that bind Ca2+. Both C2 domains interact with negatively charged phospholipids in a Ca2+ dependent …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
… The mechanism of Ca2+ action is still unknown. Synaptotagmin 1, a brain-specific vesicular transmembrane protein, is the Ca2+ sensor in neurons. It has two cytoplasmic C2 domains (C2A and C2B) that bind Ca2+. Both C2 domains interact with negatively charged phospholipids in a Ca2+ dependent …
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Development of imaging techniques to assess tumour cell death
… protein, the C2A domain of synaptotagmin-I (C2Am), which can be used to image tumour cell death in vivo using PET. C2Am binds in a Ca²⁺ -dependent manner with nanomolar affinity to phosphatidylserine (PS) on the outer leaflet of the plasma membrane of apoptotic cells or to PS …
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Molecular Determinants of Synaptic Vesicle Exocytosis and Endocytosis Coupling
… endocytosis phenotype previously reported after synaptotagmin 1 loss-of-function can also be triggered by other manipulations that promote asynchronous release such as Sr2+ substitution and complexin loss-of-function. The link between asynchronous release and slowed endocytosis was due to …
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Analysis of Synaptotagmin-SNARE Complex Interactions by One-Dimensional NMR Spectroscopy
… vesicle and plasma membrane. The protein synaptotagmin-1 is the major sensor for the calcium concentration. Synaptotagmin-1 and the SNARE complex are therefore believed to interact in order to couple the calcium concentration to membrane fusion. The detailed mechanism of the interaction is …
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Dissection of the Roles of Synaptotagmins in Calcium Dependent Neurotransmitter Release
… caused by the arrival of an action potential. Synaptotagmins (Syts) mediate this calcium-dependent regulation of neurotransmitter release by mechanisms that still remain elusive. Syts have two calcium-binding C2 domains (C2A and C2B) which are crucial for their function. Clarifying how Syt C2 …
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Synaptic vesicle protein 2A-dependent function and dysfunction at the presynapse
… proteins, synaptic vesicle protein 2A (SV2A) and synaptotagmin I (SYT1), was investigated. SYT1 predominantly acts as the Ca2+ sensor for fast synchronous release at the presynapse, whilst the exact function of SV2A remains unknown to this day. In this study, the ablation of the AP-2 binding site …
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Unraveling the Functions of Synaptotagmin and Munc13 in Neurotransmitter Release
… and Munc18-1 as core components. Munc13, synaptotagmin and complexin underlie the exquisitely tight regulation of synaptic exocytosis. The SNAREs form the SNARE complex that brings synaptic vesicles and plasma membrane together. This complex is disassembled by NSF/α-SNAP (soluble …
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Molecular Basis of Coupling Calcium Sensing to Fast Membrane Fusion by Synaptotagmin-1 in Neurotransmitter Release
The content of this work is based on the previously published articles that are cited below.