Global ETD Search
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Showing 1 to 20 of 52 for “"Synaptotagmin"”.
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Structural characterization of synaptotagmin I
Synaptotagmin I is the most abundant Ca+2 binding protein present on synaptic vesicles accounting for 7% of total vesicle protein and is widely accepted as the Ca2+ sensor in fast synchronous neurotransmitter release. The protein is composed of one trans-membrane domain, an unstructured linker …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
… The mechanism of Ca2+ action is still unknown. Synaptotagmin 1, a brain-specific vesicular transmembrane protein, is the Ca2+ sensor in neurons. It has two cytoplasmic C2 domains (C2A and C2B) that bind Ca2+. Both C2 domains interact with negatively charged phospholipids in a Ca2+ dependent …
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Mechanism of Synaptotagmin Action in Neurotransmitter Release
… The mechanism of Ca2+ action is still unknown. Synaptotagmin 1, a brain-specific vesicular transmembrane protein, is the Ca2+ sensor in neurons. It has two cytoplasmic C2 domains (C2A and C2B) that bind Ca2+. Both C2 domains interact with negatively charged phospholipids in a Ca2+ dependent …
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Molecular genetic characterization of the Drosophila synaptotagmin family
… of intense investigation in neuroscience. The synaptotagmin family of vesicle proteins has been implicated in calcium- dependent neurotransmitter release, although Synaptotagmin 1 (Syt 1) is the only isoform demonstrated to control synaptic vesicle fusion. We have characterized the six …
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Characterization of Synaptotagmin Function In Calcium Dependent Neuronal Exocytosis
Es wird vermutet, dass das Protein Synaptotagmin 1, das in der Membrane des synaptischen Vesikels sitzt, der Kalziumsensor in der neuronalen Synapse ist. Synaptotagmin 1 hat zwei sogenannte C2 Domänen, die kalziumabhängig Membranen binden. Es ist gezeigt worden, dass die Deletion von Synaptotagmin …
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Unraveling the Functions of Synaptotagmin and Munc13 in Neurotransmitter Release
… and Munc18-1 as core components. Munc13, synaptotagmin and complexin underlie the exquisitely tight regulation of synaptic exocytosis. The SNAREs form the SNARE complex that brings synaptic vesicles and plasma membrane together. This complex is disassembled by NSF/α-SNAP (soluble …
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Retrograde regulation of activity-dependent synaptic growth by Synaptotagmin 4
… to postsynaptic Ca2+ remain unknown. The Synaptotagmin family of Ca2+ sensors includes several evolutionarily conserved neuronal isoforms that are predicted to regulate vesicle fusion, including the synaptic vesicle Ca2+ sensor Synaptotagmin 1. Recently, the Synaptotagmin 4 (Syt 4) isoform …
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Analysis of Synaptotagmin-SNARE Complex Interactions by One-Dimensional NMR Spectroscopy
… vesicle and plasma membrane. The protein synaptotagmin-1 is the major sensor for the calcium concentration. Synaptotagmin-1 and the SNARE complex are therefore believed to interact in order to couple the calcium concentration to membrane fusion. The detailed mechanism of the interaction is …
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Characterization of synaptotagmin 7 function in neurotransmission and its subcellular localization at synapses
… phase of SV fusion mediated by the Ca²⁺ sensor Synaptotagmin 1 (SYT1). A slower SYT1-independent asynchronous phase is also present at many synapses and has been hypothesized to be mediated by another Synaptotagmin, SYT7. To determine if SYT7 plays an evolutionarily conserved role as an …
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Reevaluating the functional role of the C₂A domain of synaptotagmin in neurotransmitter release
… neurotransmitter. The synaptic vesicle protein, synaptotagmin, was identified as the Ca2+ sensor for this fast, synchronous neurotransmitter release. It is hypothesized that Ca2+ binding by synaptotagmin acts as an electrostatic switch. At rest, vesicles are in a state of variable priming with …
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Competition Between Synaptotagmin 1 and Complexin for SNARE Complex Binding, Controls Fast Synaptic Vesicle Exocytosis
Calcium binding to synaptotagmin 1 triggers fast exocytosis of synaptic vesicles that were primed for release by SNARE complex assembly. Besides synaptotagmin 1, fast Ca2+- triggered exocytosis requires complexins. Synaptotagmin 1 and complexins both bind to assembled SNARE complexes, but it is …
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Molecular Basis of Coupling Calcium Sensing to Fast Membrane Fusion by Synaptotagmin-1 in Neurotransmitter Release
The content of this work is based on the previously published articles that are cited below.
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Genetic analysis of stoned B/stonin 2 function in vivo
… ist und u. a. von Clathrin, Dynamin, AP2 und Synaptotagmin 1 abhängig ist. Stonin 2 ist ein Protein, das in Hirngewebe angereichert vorgefunden wird und sowohl in axonalen synaptischen Vesikel Clustern in Neuronen als auch in Clathrin/AP2 umhüllten Membraneinstülpungen in Astrogliazellen …
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Structural and Functional Studies of C2-Domain Proteins Involved In Neurotransmitter Release
… properties of two types of C2-domain proteins, synaptotagmins and RIMs. With NMR spectroscopy and biochemical assays, we demonstrated that synaptotagmin 4 is a Ca2+ sensor in Drosophila but not in rat, in contrast to the prediction based on sequence alignments. X-ray crystallography revealed …
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Calcium Triggered Synaptic Vesicle Exocytosis
… substitution, namely D232N and D238N in synaptotagmin-1, combined with electrophysiology, I found evoked release in D232N mutant neuronal cultures is significantly increased, whereas in D238N cultures release is slightly but significantly decreased. Ca 2+ titration curves indicated the …
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Regulation of SNARE-Mediated Synaptic Vesicle Release by Synaptotagmins and Complexins
… release. Among these proteins are synaptotagmins and complexins, two protein families that directly interact with the SNARE complex, and that are interdependent to each other in regulating SNARE-mediated synaptic vesicle release: complexin clamps neurotransmitter release until …
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SNAREs in evoked and spontaneous neurotransmission
… konnten wir zeigen, dass der knock-out von Synaptotagmin-7 keinen Phänotyp in hippocampalen Neuronen besitzt. Es konnte ebenso gezeigt werden, dass SNAP-23 in der Tat mit Synaptotagmin-7 in einer lebenden Zelle interagiert, und auch, dass Synaptotagmin-7 der mutmaßliche Kalzium-Sensor für …
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Structure and Function of Proteins Involved in Regulated Secretion: Synaptotagmins and Complexin
… neurotransmitter release such as Complexin, and Synaptotagmin as well as other required proteins such as Munc13 and Munc18. Complexin is a small soluble protein that binds to the SNARE complex with high affinity and regulates the formation of the SNARE complex. Synaptotagmin is the calcium sensor …
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Molecular Determinants of Synaptic Vesicle Exocytosis and Endocytosis Coupling
… endocytosis phenotype previously reported after synaptotagmin 1 loss-of-function can also be triggered by other manipulations that promote asynchronous release such as Sr2+ substitution and complexin loss-of-function. The link between asynchronous release and slowed endocytosis was due to …
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