Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"Synaptosomes."”.
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Quantitative Proteomics Analysis of Differentially Expressed Proteins in Aβ(17-42) Treated Synaptosomes
… oxidative stress levels in Aβ(17-42) treated synaptosomes and control. By using proteomics, we have also identified several biomarkers including aldehyde dehydrogenase, aldolase, α-enolase, heat shock cognate 71, peptidyl-prolyl cis-trans isomerase, and ATP synthase α chain. Our present …
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In vitro release of endogenous monoamine and amino acid transmitters from rat brain synaptosomes
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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In vitro activation of high-affinity choline uptake in synaptosomes prepared from hippocampus and striatum of rat brain
This document only includes an excerpt of the corresponding thesis or dissertation. To request a digital scan of the full text, please contact the Ruth Lilly Medical Library's Interlibrary Loan Department (rlmlill@iu.edu).
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Onset of Insulin Resistance in Hippocampal Synapses After Traumatic Brain Injury: Relevance to Alzheimer’s disease and Therapeutic Implications
… insulin responsiveness in isolated hippocampal synaptosomes after TBI. In addition to assessing both Aβ and tau binding on synaptosomes, I performed electrophysiology at the intermediate and chronic time-points to assess the dysfunctional impact of Aβ and tau oligomers as well as the protective …
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Dynamins and myosin-II regulate the distinct modes of synaptic vesicle exocytosis in mature cerebrocortical nerve terminals and this involves calcium dependent phosphorylations.
… for distinct modes of SV exocytosis. Herein, synaptosomes, pinched off nerve terminals, prepared from cerebral cortex of adult male Wistar rats were used as a model system to investigate these processes. Especially, A. Ashton had previously demonstrated the existence of KR mode of exocytosis …
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Microglial Phagocytosis of Bacteria and Synapses
… and galectin-3 opsonised isolated synapses, or ‘synaptosomes’, in a similar fashion to the bacteria, suggesting diverse regulatory functions for these proteins. Microglial phagocytosis of synaptosomes was also enhanced by apolipoprotein E and extracellular tau, which may play a role in …
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Phosphorylation sites on specific neuronal proteins can control the mode of synaptic vesicle exocytosis and thereby regulate synaptic transmission
… by Kiss-and-Run (KR) mechanisms. In this thesis, synaptosomes prepared from adult rat cerebrocortex demonstrated that these modes can be switched by regulating the intracellular calcium levels and/or protein phosphorylation reactions. The stimuli employed were: 30 mM K+ (HK), 1 mM 4-aminopyridine …
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Brain distribution and release of Cholecystokinin octapeptide
… was homogenised to shear off nerve terminals (synaptosomes) which were purified and used to study the release of the peptide from hypothalamic and extrahypothalamic nerve endings.
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The role of protein phosphorylation in the switching between modes of synaptic vesicle release
… on the presynaptic nerve terminal - by employing synaptosomes - for understanding the mechanisms occurring in synaptic vesicle exocytosis. Synaptic vesicles have rapid recycling capabilities and this in part may be due to a kiss-and-run mode of neurotransmitter release. To investigate the mode of …
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The glutamate hypothesis of depression: the effect of stress and glucocorticoids on glutamate synapse and the action of antidepressants
… induces an increase of glutamate release from synaptosomes of prefrontal and frontal cortex (PFC/FC), via glucocorticoid receptors (GR) activation and SNARE complex accumulation in synaptic membranes. Furthermore, we have demonstrated that the increase of glutamate release induced by acute …
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Hdac6 Inhibition Reverses Long-Term Doxorubicin-Induced Cognitive Dysfunction By Restoring Microglia Homeostasis
… in mitochondrial function or morphology in brain synaptosomes, myelination in the cingulate cortex using Black Gold II staining, or changes in astrocyte reactivity as assessed by anti-GFAP immunofluorescence staining. Using advanced imaging techniques and single-nucleus RNA sequencing, I …
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Serotonergic and dopaminergic systems as targets for exogenous neurotoxins causing a parkinsonian syndrome
… several aspects of amine uptake in mouse brain synaptosomes. Both MPTP and MPP⁺ were inhibitors of labeled neurotransmitter (serotonin and dopamine) uptake consistent with previous studies. MPP⁺ had a higher inhibitory potency in the dopaminergic system, while MPTP had a higher inhibitory …
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MacMARCKS and the Protein Kinase C Signal Transduction Pathway: Role in Neural Secretion and Neural Development
… in a stimulus-dependent manner in rat brain synaptosomes and PC12 cells. It is localized to cell bodies and neurite tips of PC12 cells as well as being present on synaptic vesicles. A dominant-negative mutation in the MacMARCKS effector domain which abolishes PKC-dependent phosphorylation is …
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Shedding Light On General Anesthesia: Uncovering The Molecular Mechanisms For Propofol And Volatile Anesthetics
… The range of propofol-binding proteins within synaptosomes was further defined with the synthesis of a novel photoaffinity tandem click chemistry-active ligand and the development of a quantitative affinity-based protein profiling workflow. Results of the investigation indicated a highly …
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Biochemical differences in the modes of synaptic vesicle release between control and streptozotocin-induced diabetic rats and possible relationship to changes in behaviour
… has been studied herein, using cerebrocortical synaptosomes from adult rats. Switching between the two modes depends upon the secondary messenger calcium and protein phosphorylation reactions. Dr. Ashton has previously demonstrated that an increase in intracellular calcium levels regulates the …
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Persistent “hijacking” of brain proteasomes in HIV-associated dementia
… Analysis of isolated nerve endings, or synaptosomes, revealed immunoproteasome increases in synapses of HIV-positive subjects that correlated with increases in 14-3-3 zeta and decreases in synapsin 1. These findings suggest “hijacking” of constitutive proteasomes by immunoproteasomes …
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Synaptic dysfunction, tau spreading, and cognitive resilience through the lens of tau oligomers in Alzheimer’s disease and related dementias
… binding and internalization of tauO into human synaptosomes via specific membrane protein pathways, suggesting a mechanism for Aβ-facilitated trans-synaptic spread of tau pathology. Chapter 3 reveals that distinct tau species exert region-specific effects on the synaptic excitation/inhibition …
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Neuropathological significance of PLD1 in Alzheimer's disease and behavioral variant frontotemporal dementia
… first time, an elevated PLD1 expression in crude synaptosomes from AD post-mortem brains and further demonstrated the effect of amyloidogenic insults amyloid-β (Aβ) and tau in wildtype mice, implicating a role for PLD1 in synaptic deficits. Given this background, I implemented a 12-month-old …
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The Characterization Of Pb2+ Toxicity In Rat Neural Development: An Assessment Of Pb2+ Effects On The Gaba Shift In Neural Networks And Implications For Learning And Memory Disruption
… approaches), physiological cellular components (synaptosomes and protein expression) and finally through learning and memory assessment with GABA mimetic drug manipulations in the intact animal (behavioral pharmacology). The influence of a 956ppm Pb2+ gestational diet (i.e. from birth to …
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Dynamic Measurement Of Soluble Human Aβ In A Combined Microdialysis-Experimental Traumatic Brain Injury Mouse Model
… following injury. To test this, we prepared synaptosomes in sham and injured PDAPP mice and measured levels of Aβ and APP by ELISA. No significant differences between sham and 2.0 mm-injured mice were detected. Future experiments will determine whether enhanced clearance accounts for …
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