Global ETD Search
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Showing 1 to 19 of 19 for “"Synapsis"”.
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Epic Search for the Perfect Partner: MutSgamma Promotes Efficient Meiotic Recombination and Homolog Pairing in Mouse Spermatocytes
… to analyze pairing in organisms by cytology, synapsis is used as a poor proxy for pairing. Synapsis occurs when homologs are brought in closer juxtaposition and connected by a proteinaceous structure called the synaptonemal complex. Synapsis is achieved much later in meiotic prophase than …
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Investigating the control of pairing and crossover formation in meiosis of Arabidopsis thaliana
… as non-crossovers, important for pairing and synapsis. How these processes are integrated and controlled remains poorly understood. Telomeres are thought to play a role in pairing of homologues. The role of telomeres was investigated in Arabidopsis by treatment with colchicine, a …
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MODELING INFERTILITY: SINGLE GENE MUTATIONS AND CONSEQUENCES FOR GERM CELL DEVELOPMENT
… arrest in pachynema and exhibit defective synapsis. TRIP13 localization in other mutant strains with defective synapsis, indicated that TRIP13 is likely recruited by a component of the SC during early synapsis. In Chapter 3, we examined CCNB3, a meiosis-specific cyclin, which is required …
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Higher Order Chromosome Organization and Recombination Dynamics of Meiotic Prophase I In Mouse Spermatocytes
… parental chromosomes to find each other (pairing/synapsis) and to exchange genetic information thus allowing faithful segregation of chromosomes and the production of haploid gametes. At the start of meiotic prophase I, meiotic chromosomes organize into loop arrays that extrude out of the …
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DNA-PKcs/ Artemis Complex in DNA Double-Strand-Break Repair: Cryo-EM and Biochemical Studies
… into three steps - DNA end recognition, end synapsis and processing, and end ligation. The main objective of my project is to understand the interaction in the complex between DNA-PKcs and Artemis, which is the major nuclease in the step of end synapsis and processing to help clean up the …
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Prenatal exposure to di-(2-ethylhexyl) phthalate and high-fat diet synergistically disrupts mouse fetal oogenesis and affects folliculogenesis
… out to monitor meiotic gene silencing and synapsis in E18.5 oocytes, after making meiotic chromosomal spreads. At 8 days postpartum (dpp) and 21 dpp, F1 ovaries were harvested and subjected to histological evaluation. We found that treatment with an environmentally relevant dosage of DEHP …
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Meiotic defects in infertile men
… used immunofluorescent techniques to observe the synapsis and recombination of chromosomes during meiosis, and fluorescent in-situ hybridization (FISH) to assess sperm aneuploidy. We analyzed testicular tissue from thirty-one men (10 fertile and 21 infertile men). We observed that ~36% (5/14) of …
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The role of SYN1 in early Arabidopsis meiosis
… I, suggesting that both axis formation and synapsis are compromised. Distribution of the recombination proteins AtRAD51 and AtMLH1 was also aberrant. Localization of SYN1 in wild-type nuclei revealed a continuous signal along the chromosome axes. However, careful inspection revealed that …
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Isolation in Tragopogon (Compositae)
… chromosome homologies by determining whether synapsis was regular in the spore mother cells. (4) Paper chromatographic analysis of amino acids and secondary substances (flavonoids) was performed to see if chemical similarities and dissimilarities exist among the Tragopogon taxa studied. (5) …
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A molecular characterization of the meiotic initiation factor STRA8 in mice
… segregation depends on the pairing and synapsis of homologous chromosomes and crossing over during prophase of meiosis I. These chromosomal events are generally conserved across eukaryotes and have been extensively studied. In contrast, the regulation of meiotic initiation is less …
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Microfluidics and chemical kinetics to analyse protein interactions, aggregation, and physicochemical properties
… plaques, which surround neuronal cells impeding synapsis. The number of AD patients is increasing, but a cure has not been founded yet. Therefore, it is crucial to investigate the mechanisms underlying amyloid aggregation and screening for compounds able to prevent this irreversible process. …
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Equine Spermatogenesis: Meiotic Chromosome Behavior and Recombination
… sperm parameters as well as investigating the synapsis and recombination frequency and distribution, at PI or MI, would assist with predictions of stallion fertility prior to breeding. In addition, this study will enable investigators to use linkage analysis in identifying and localising …
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Development of Novel Technologies to Investigate DNA Double-Strand Break Repair Uncovers a Role for the ATM Kinase in Error-Free NHEJ with Implications for Neurodegenerative Diseases
… redundant manner with the core NHEJ-mediated end synapsis machinery. Second, we propose that ATM promotes EF-NHEJ via promoting R-loop resolution by both SETX and ERCC6L2. We show that the role of ATM in promoting EF-NHEJ is largely independent of MRN-dependent ATM activation, and completely …
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Analyse des meiotischen Cohesinkomplexes in Drosophila melanogaster-Weibchen
… reduziert, was Auswirkungen auf die Paarung und Synapsis der homologen Chromosomen hatte. Mit Hilfe von 2-Hybridinteraktionsstudien in der Hefe konnten darüber hinaus auch noch Wechselwirkungen von SOLO mit verschiedenen Cohesin-assoziierten Proteinen, wie zum Beispiel dem Cohesinladeprotein …
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Expression and function of basigin during early pregnancy and spermatogenesis
… displayed normal homologous chromosome synapsis and progression to the midpachytene stage. Both meiosis I (MI) and meiosis II (MII) spermatocytes were detected in the KO preparations. However, expression of the acrosome marker SP-10 was extremely low in germ cells of Bsg KO mice …
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Cryo-EM as a Tool to Study the Diverse Interactome of Ku 70/80 in NHEJ and Beyond
… form the DNA-PK holoenzyme complex, to mediate synapsis of the broken DNA ends (7, 8). Depending on the DNA end configuration, DNA end processing can follow by specialised processing enzymes, nucleases, and DNA polymerases to ensure ends are ready for ligation (9). Ligation is mediated by the …
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Apareamiento intergenómico y formación de polen no reducido en híbridos trigo x aegilops
… hybrids, GISH reveals the outcome of synapsis and crossing-over between the constituent genomes. Metaphase-I pairing of different Triticum×Aegilops hybrids has been analyzed by in situ hybridization experiments that combined both total genomic DNA probes and repeated DNA probes, …
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Building a bridge between biophysics and neurobiology: A synergic approach to develop Alzheimer’s disease translational models and research tools
… with dysfunctional phenotypes such as altered synapsis and hypermetabolism. The research presented in Chapters 3, 4, and 5, revealed that Aβ aggregates in the developed cell models mostly co-localize with cell membranes and progress from spherical to irregular, fibrillar structures over time. …