Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 124 for “"Surface protein"”.
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Probing surface protein patterning in biological systems using fluorescence nanoscopy
… nanoscopy to study the patterning of proteins in biological surfaces at a high spatial resolution of 25 nm. We also developed new analytical methods to extract quantitative information on protein arrangement within biological substrates to elucidate key phenomenon such as signal …
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Identification of surface protein complexes of Streptococcus pyogenes through protein microarray technology
… composition and structure of the bacterial cell-surface proteome and its complexes can provide an invaluable tool for its comprehensive understanding. The knowledge of protein complexes composition and structure could offer new, more effective targets for a more specific and consequently …
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Analysis of the role of outer surface protein C (OspC) in Borrelia burgdorferi pathogenesis
… cycle is through the expression of outer surface protein C (OspC). OspC is a surface exposed lipoprotein encoded on circular plasmid 26 that forms homodimers on the bacterial surface and has distinct conserved and variable portions of sequence. When ospC is deleted, the spirochetes are …
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External Cell Surface Protein Phosphorylation in Normal and Rous Sarcoma Virus Transformed Chick Embryo Fibroblasts
<p>Endogenous protein kinase activity was detected on the external cell surface of both normal and Rous sarcoma virus (RSV)-transformed chick embryo fibroblasts (CEF). Cells growing in plastic dishes were incubated with [Υ-³²P]ATP for 20 minutes. Under the conditions employed, only proteins located …
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Entwicklung eines Lipoprotein-Impfstoffes aus Pflanzen : Produktion des rekombinanten 'outer surface protein A' (OspA) von Borrelia burgdorferi in Tabakchloroplasten
… Stellenwert bei der Produktion therapeutischer Proteine, besonders von Impfstoffen, ein. Einige dieser Proteine benötigen für ihre Funktionalität verschiedenste post-translationale Modifikation und es ist nicht bekannt, ob Pflanzen zu diesen Stoffwechselleistungen befähigt sind. In der …
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A Molecular Genetic Investigation of the Regulation of Outer Surface Protein C in Borrelia Burgdorferi: Identification and Characterization of the Novel Regulatory Protein BBD18
… and subsequently alter their gene expression and protein profiles. As part of this adaptation, spirochetes within the tick midgut downregulate outer surface protein (Osp) A and upregulate OspC. Although OspC is an essential B. burgdorferi virulence factor needed for early mammalian infection, it …
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A Molecular Genetic Investigation of the Regulation of Outer Surface Protein C in Borrelia Burgdorferi: Identification and Characterization of the Novel Regulatory Protein BBD18
… and subsequently alter their gene expression and protein profiles. As part of this adaptation, spirochetes within the tick midgut downregulate outer surface protein (Osp) A and upregulate OspC. Although OspC is an essential B. burgdorferi virulence factor needed for early mammalian infection, it …
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Regulation of outer surface lipoprotein A in the Lyme disease spirochete Borrelia burgdorferi
… undergoes dramatic alterations in its surface lipoprotein. Two essential lipoproteins, outer surface protein A (OspA) and outer surface protein C (OspC), are reciprocally regulated throughout the B. burgdorferi lifecycle. Very little is known about the regulation of OspA. These studies …
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A Study of Cell Surface Change After Viral Transformation by Iodination and Cell-Cycle Dependent Phosphorylation of Sv-40 Large Tumor Antigen
… parts. Part I deals with iodination of the cell surface protein before and after viral transformation and Part II deals with cell-cycle dependent phosphorylation of the large tumor antigen induced by SV-40 in human cells.
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Investigation of the Interaction of Modified Soy Protein Fractions With Wheat Starch Granule Components
… wheat products with a significant soybean protein addition, a thorough understanding of each product's individual physicochemical, structural and dynamic nature is necessary. Significant research has been conducted on the function of wheat gluten in bakery products. This information has …
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ISOLATION AND CULTURE OF FIBROBLASTS FROM ENDOSCOPIC DUODENAL BIOPSIES OF CELIAC PATIENTS
… (Q-banding analysis), expression of typing proteins (fibroblast surface protein and cytokeratin 20) and morphometric parameters (diameters and their ratio, perimeter, area and perimeter/area ratio at computerised image analysis) were investigated on cultured cells. Results. Primary cells …
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Utilization of Cholera Toxin B as an Adjuvant for the Subunit PspA Vaccine, Provides Effective Protection against Streptococcus pneumoniae Challenge
… One such proponent is known as Pneumococcal Surface Protein A (PspA) which is a surface protein conserved throughout most serotypes and a potential candidate as a subunit vaccine against <em>Streptococcus pneumoniae. </em>To increase the immunogenicity of PspA for long term and effective …
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Microsporidia Spore Adherence and Host Cell Infection In Vitro
… of new therapies. Spores adhere to host cell surfaces<em> in vitro. </em>Our laboratory has focused on determining specific host cell and microsporidia spore surface participants in spore adherence. Our previous studies have shown that host cell sulfated glycosaminoglycans and the spore …
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Characterization of a single-chain anti-T cell receptor antibody and a single-chain T cell receptor
The T cell receptor (TCR) is a cell surface protein that specifically binds to a foreign antigen on the surface of another cell. The TCR is made up of two disulfide-linked chains (called $\alpha$ and $\beta$) each containing a variable and a constant domain. The tertiary structure and binding …
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Tending towards Non-fouling: A study of the interaction between proteins and surfaces prepared by radio frequency glow-discharge plasma
All proteins adsorb irreversibly onto surfaces and often results in denaturation, aggregation and loss of activity. This has serious implications not only for medical devices that are used clinically for the treatment of disease but also for the pharmaceutical industry that deals with handling, …
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Development of methods for the detection Staphylococcal Protein A in ICU patient urine
… in the Intensive Care department. Staphylococcal Protein A (SpA) is an immunoglobulin-binding surface protein, expressed by more than 98.9% of S. aureus strains, with a primary function of aiding S. aureus in the colonisation of the host. While many Staphylococcal toxins are excreted by the …
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Single-cell Protein-Transcriptome Atlas of Haematopoiesis across the Human Lifespan
… expression and function. Studying genes and cell surface proteins at the single-cell level using a combination of scRNA-seq and index sorting has proven valuable for inferring individual cellular states and identifying subpopulations for further research. Nevertheless, traditional methods that …
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Regulation of Surface Proteins Assembly on the Wall of Gram-Positive Bacteria
<p>Cell wall peptidoglycan-anchored surface proteins are essential virulence factors in many Gram-positive bacteria. Attachment of these proteins to the peptidoglycan is achieved through a transpeptidation reaction, whereby sortase cleaves a conserved Cterminal LPXTG motif and attaches the protein …
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The characterisation of the cell surface of Staphylococcus aureus in the search for new therapeutic targets
… cell receptors, a better understanding of which proteins are displayed on the cell surface is required. This dissertation presents an analysis of the surface proteome of S. aureus and describes several potential novel adhesins. Enzymatic cell shaving and surface protein biotinylation were used to …
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Hypervirulent Clostridium Difficile Strains: Adherence, Toxin Production and Sporulation
… more adherent than other strains. The bacterial surface protein SlpA varied in both size and amount between strains. Both total <italic> C. difficile </italic>surface protein preparations and specific antibodies to SlpA significantly reduced adherence, indicating that SlpA specifically …
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