Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Surface biotinylation"”.
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Holin-dependent secretion of a large clostridial cytotoxin by C. perfringens
… using FAST fluorescence technology and cell-surface biotinylation to determine if the amphipathic helix of TpeE changes its orientation in the presence of TpeL. Using FAST fluorescence, the mean pixel intensity for each frame did not change between TpeE-FAST and TpeE-FAST TpeL cultures, using …
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Distribution and targeting of CLC-3
… localized to the plasma membrane as assessed by surface biotinylation. Plasma membrane ClC-3 was rapidly endocytosed with t1/2 of approximately 9min. Biotinylation experiment detected a portion of ClC-3 recycled back to the cell surface. Antibody binding to an external HA epitope demonstrated …
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Dynamic subcellular proteomics identifies novel regulators of adipocyte insulin action
… driven changes, we conducted a targeted cell-surface biotinylation proteomic study, enhancing the resolution of plasma membrane specific changes. This study identified additional novel insulin-stimulated protein translocation events (TSPAN31, SLC20A2, CD302) and confirmed translocation events …
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Regulation Of Nkcc2 Trafficking By Vesicle Fusion Proteins Vamp2 And Vamp3 In The Thick Ascending Limb
… proteins VAMP2 and VAMP3 blunts the increase in surface NKCC2 expression and NaCl absorption in response to stimulation by cAMP. In other cells, VAMPs mediate fusion of exocytic vesicles with the plasma membrane. Whether VAMP2 and VAMP3 mediate different pathways for NKCC2 exocytic delivery to …
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Influence of Sodium Channel Activation on NMDA Receptor-Mediated Structural Plasticity.
… Acute exposure to PbTx-2 increased cell surface expression of NR2B containing NMDARs and TrkB receptors (high affinity receptor for Brain-derived neurotrophic factor, BDNF) as observed in the surface biotinylation assay, demonstrating direct evidence for the ability of PbTx-2 to sensitize …
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Role of Neuraminidase-mediated Desialylation in Microglial Activation
… is desialylation which is the removal of cell surface sialic acid residues by enzymes called sial- idases or neuraminidases. Sialic acids are 9-carbon, negatively charged monosaccharides that normally form the terminal residue of the mammalian cells’ glycocalyx. A murine microglial cell line …
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Novel Role of ErbB-2 in Inhibition of Jagged-1-Mediated Trans-Activation of Notch in Breast Cancer
… of SKBr3 cells</p><p>increased the cell surface protein expression of Jagged-1 by flow cytometry and cell surface biotinylation. Moreover, confocal studies indicated that Jagged-1 and Notch-1 co-localized possibly in early endosomal antigen-1 (EEA-1) positive vesicles. However, upon …
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The subcellular trafficking of the prion protein: Characterisation of the function of the PrPc N-terminus
… was analysed. Metabolic labelling and surface-biotinylation assays were performed in order to compare the intracellular trafficking and turnover of PrPc mutants showing specific deletions within their N-terminal sequence with those of wild type PrPc (wtPrPc). Upon transient expression …
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The mechanisms of aquaporin expression and translocation in cerebral pathologies
… of the AQP4 water channel. Modulating AQP4 surface localisation and expression rather than direct blocking of its pore could provide novel therapeutic benefits. The research in this thesis primarily elucidates the regulatory mechanisms involved in processes of hypotonicity and hypoxia …
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The characterisation of the cell surface of Staphylococcus aureus in the search for new therapeutic targets
… of which proteins are displayed on the cell surface is required. This dissertation presents an analysis of the surface proteome of S. aureus and describes several potential novel adhesins. Enzymatic cell shaving and surface protein biotinylation were used to catalogue proteins on the cell …
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Cell Wall/Surface Proteome of Candida albicans: its Application in Rapid Identification of Yeast Species by Mass Signature and Characterization by in vitro and in vivo Chemical Labelings
… impaired (such as those in HIV patients). Cell surface of C. albicans is the frontier where initial interplay between host-pathogen takes place and therefore is of great importance in understanding the mechanism of hostpathogen interaction. MALDI-TOF-MS analysis of intact fungal cells yielded …