Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 93 for “"Surface Plasmon Resonance (SPR)"”.
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The Development of an Aptamer-based Surface Plasmon Resonance (SPR) Sensor for the Real-time Detection of Glycated Protein
… the feasibility of using an aptamer-based surface plasmon resonance (SPR) sensors for the real-time detection of glycated blood proteins (i.e., GA) has been studied. For the purpose of establishment, the well documented thrombin binding aptamers were immobilized onto a gold sensing surface …
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Preparation of Heparin Surface for Quantification of Fibroblast Growth Factor-2 (FGF-2) Binding Using Surface Plasmon Resonance (SPR)
… interactions using a Reichert Inc, SR7000, surface plasmon resonance (SPR) instrument. The heparin was attached via streptavidin-biotin linkage where the streptavidin was covalently coupled to the mSAM and biotinylated heparin bound to it. These chips were then used to quantify the …
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Numerical characterisation of label free optical biosensors
… penetration depth achieved by techniques like Surface Plasmon Resonance (SPR) and sensor architectures based on nanowires and slot waveguides. This thesis presents three different architectures of label-free optical biosensors. First, a fiber optic surface plasmon resonance (SPR) biosensor for …
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Worm-shaped polymersomes and their effects on targeting efficiency
… onto target substrates were assessed using surface plasmon resonance (SPR) spectroscopy and adherence of the polymersomes to a target cell layer was also evaluated using flow chamber simulating blood flow.
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Interfacial Properties of Environmentally Responsive Polymers
… the mucoadhesive properties using SFA and Surface Plasmon Resonance (SPR), and identify key parameters that control the interaction between hydrogen bonding polymers and soluble mucin.
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Adsorption of Blood Proteins onto Polysaccharide Surfaces
In this study, surface plasmon resonance (SPR) and quartz crystal microbalance with dissipation monitoring (QCM-D) were combined to investigate the adsorption behavior of two platelet adhesion-related blood proteins, human serum albumin (HSA) and human serum fibrinogen (HSF), on two polysaccharide …
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Structural Characterization of Sulfur-Based Monomolecular Thin Films: I. Langmuir-Blodgett Films Derived From Multisulfur Heterocycles. II. Second Harmonic Generation Studies of Alkanethiolates on Ag and Au
… (SHG) has been used as a very sensitive surface probe to study metal-electrolyte interfaces at potentials within the double layer charging region. In this work, the potential dependence of second harmonic (SH) signal from metal/thin film/electrolyte systems have been studied. …
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Using multiple layers and surface roughness control for improving the sensitivity of SRP sensors
Surface plasmon resonance (SPR) sensors have been developed quickly in the past twenty years in biosensing. However, the sensitivity of them restricts them from small molecular detection. This thesis focuses on the sensor chips of the SPR sensors and presents two potential methods to improve the …
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The characterisation of the peanut agglutinin an evolved plant lectin, with improved specificity to the Thompson Freidenriech antigen
… states and patterns of glycosylation on cell surfaces and suitably engineered lectins may be able to recognise tumour specific carbohydrate structures. This study was aimed at carrying out the biophysical characterisation of a set of PNA mutants which showed apparent improvement in specificity …
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Real Time Biological Threat Agent Detection with a Surface Plasmon Resonance Equipped Unmanned Aerial Vehicle
… that made UAV integration a feasible task. A Surface Plasmon Resonance (SPR) biosensor was integrated for the first time into a small UAV platform, allowing the UAV platform to collect and then quantify the concentration of an aerosolized biological agent in real-time. A sensor operator ran …
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Nanobiossensor a fibra óptica revestido com filme fino de ouro para detecção da bactéria Escherichia coli
… by two effects: bending loss by refraction and Surface Plasmon Resonance (SPR). Bacterial selectivity was obtained by specific antibodies immobilization on the fiber surface. Sensors coated with several thicknesses of gold thin film were tested in order to improve bacteria adhesion and to enable …
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External cavity laser biosensor for label-free detection
… for drug discovery process. We develop a plasmonic ECL to further improve the performance metrics of ECL biosensors. Utilizing surface plasmon resonance (SPR) biosensor as mode-selection filter, the plasmonic ECL demonstrates 3× higher sensitivity than photonic crystal based ECL biosensor. …
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Self-Assembly of Pullulan Abietate on Cellulose Surfaces
… lignin-carbohydrate complex (LCC) and a model surface for cellulose fibers was used. The self-assembly of the polysaccharide pullulan abietate (DS=0.027) onto a regenerated cellulose surface prepared using the Langmuir-Blodgett (LB) technique was studied via surface plasmon resonance (SPR). …
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Self-Assembled Monolayers of N-Heterocyclic Carbenes on Gold and Silver: Applications in Biosensing
Modification of metallic surfaces using thiol-based self-assembled monolayers (SAMs) has been explored in the literature in the past three decades; silver surfaces less explored than gold. This technology has promise in many surface-based applications, specifically in micro electronics, sensors …
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The synthesis and characterisation of Langmuir-Blodgett film forming TCNQ adducts.
… infrared spectroscopy (RAIRS) and surface plasmon resonance (SPR).A number of N-alkylpyridinium benzimidazolate betaine derivatives and the related betaine-TCNQ adducts were prepared and their LB film forming properties were studied.
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A Kinetic Study of Anti-VEGF-A Polyclonal Antibodies and Anti-VEGF-A ssDNA Aptamers
… endothelial growth factor A (VEGF-A) using Surface Plasmon Resonance (SPR). It was hypothesized that the anti-VEGF-A aptamer had the same, if not better, binding kinetics than the anti-VEGF-A polyclonal antibody, and as such offers an ideal replacement for use in in field, real-time testing …
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Eukaryotic protein mimicry as an infection strategy for Ehrlichia chaffeensis
… the TRP120 multifunctional effector on the surface of infectious dense-cored ehrlichiae which is known to play a role in phagocytic entry, but a cognate host receptor has not been identified. We recently reported that E. ch. activates canonical Wnt signaling in monocytes to promote bacterial …
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Electrochemical impedance spectroscopy and surface plasmon resonance for diagnostic antibody detection
… is high. Evanescent field biosensing, e.g. Surface Plasmon Resonance (SPR), and electrochemical detection, in particular Electrochemical Impedance Spectroscopy (EIS), have been shown viable for detection of lower affinity antibodies, based on the principle that these technologies allow the …
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Modeling and Simulation of Optical Properties of Noble Metals Triangular Nanoprisms
… community for its applications arising from its plasmonic properties. The optical properties achieved by these materials via excitation of plasmons is very unique to these materials and used as diagnostic and therapeutic agents in the field of medicine, and as sensors in a gamut of disciplines …
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Quantification of the Binding of Insulin-like Growth Factor-I (IGF-I) and IGF Binding Protein-3 (IGFBP-3) Using Surface Plasmon Resonance
… of mammalian cells through the IGF-I cell surface receptor (IGF-IR). A unique feature of the IGF-I system is the regulation of this binding by soluble IGF binding proteins. Recent studies from our laboratory show that there is a pH dependence in the association of IGF-I with the cell …
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