Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Suppression Subtractive Hybridization"”.
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Differential Expression of Genes During Diapause in the Flesh Fly, <em>Sarcophaga crassipalpis</em>.
… crassipalpis</em>. The results of a Suppression Subtractive Hybridization procedure was used to indentify genes that are differentially regulated in both diapause and nondiapausing states while suppressing genes that are common to both states. Randomly picked colonies from both …
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The analysis of gene transcripts associated with conidiation in the insect pathogenic fungus, Metarhizium anisopliae /
… hi this study we used the PCR-based technique, suppression subtractive hybridization (SSH) to selectively amplify conidial-associated genes in M. anisopliae. We then identified the presence of these differentially expressed genes using the National Center for Biotechnology Information database. …
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Analysis of target genes of the transcription factor CREM during mouse spermatogenesis
… of CREM-deficient mice and wild-type mice. Using suppression subtractive hybridization we have picked 12,000 clones with sequence fragments of genes expressed stronger in wild type then in the CREM -/- mutant. 956 unique sequences were characterised. Homologies to 158 known mouse genes, 99 other …
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Bioinformatic and molecular approaches for the analysis of the retinal pigment epithelium (RPE) transcriptome
… was constructed in-house using a PCR-based suppression subtractive hybridization (SSH) technique (Diatchenko et al., 1996, 1999), which normalizes for sequence abundance and achieves high enrichment for differentially expressed genes. CAP3 (Huang and Madan, 1999) was used to assemble the …
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Analysis of transcription factor CREM dependent gene expression during mouse spermatogenesis
… mice and wild-type mice were compared using the suppression subtractive hybridization (SSH) technique as well as oligonucleotide DNA microarrays. SSH was used to selectively amplify the differentially expressed genes. 12,000 clones, which contain sequence fragments of genes expressed stronger in …
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The Molecular Biology of Lichen Symbiosis and Development
… of a stratified lichen thallus. Using suppression subtractive hybridization to determine differential gene expression, fungal and algal libraries were made of upregulated genes in the first 2 stages of lichen symbiosis. The symbiotic expression levels of select genes were then verified …
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Molecular dissection of Alternaria brassicicola pathogenesis mechanisms
… candidate pathogenicity genes by creating two suppression subtractive hybridization (SSH) libraries enriched for A. brassicicola genes during fungal pathogenesis, and one full length cDNA library of genes expressed during nitrogen starvation. The first library was created to be enriched for …
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Cloning and functional characterization of novel genes expressed preferentially in the human retina
… retina specific transcripts was generated using suppression subtractive hybridization (SSH) technique. In total, 1113 clones were randomly isolated from the retina SSH cDNA library and partially sequenced. On the basis of BLASTN algorithm analysis these clones were classified into four categories …