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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Supported lipid bilayer"”.
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Polymer Supported Lipid Bilayer Membranes for the Integration of Transmembrane Proteins
This work reports on the successful formation of supported multicomponent lipid bilayer membranes (sLBMs) from natural occurring lipids as well as synthetic lipids on a set of polymer cushions consisting of alternating maleic acid copolymers. Maleic acid copolymers provide a versatile platform to …
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Development of a Biosensor for Investigating Membrane Curvature Sorting
… of cellular membranes plays a critical role in lipid and protein sorting. A novel biosensor was developed to probe the influence of curvature on sorting. This biosensor mimics large, two-dimensional membranes in dynamic equilibrium, achieves high spatial resolution between curvature and …
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Bioelectronic and microscopic analysis of bacterial membrane systems with an applied focus on antimicrobial development
… precision. The first is the development of the supported lipid bilayer model that represents bacterial cell membranes in an *in vitro* setting. These models have existed for some years, but recently a breakthrough has been achieved where supported lipid bilayers can now be created which contain …
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The Membrane Topology of the Microsomal Cytochromes P450: Interfacial Characterization of Protein Structure
… two truncated P450s with a phosphatidylcholine supported lipid bilayer. The data has been fit to a kinetic model that incorporates a semi-empirical available surface function to account for the effect of steric interactions between pre-adsorbed molecules. The results indicate that P450 does in …
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Microscopic and Spectroscopic Investigation Of: I. Copper-Additive Systems in Electroplating Bath. II. Supported Phospholipid Bilayer Systems
… used to observe the phase transition process of supported lipid bilayer systems with and without the influence of other macromolecules. Addition of a weak polyelectrolyte, poly(methacrylic acid) (PMA), to a supported phospholipid bilayer depresses the melting temperature and alters the morphology …
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Modeling interactions between cells and the aqueous environment
… the cell membrane model consists of a hydrogel-supported lipid-bilayer. Hydrogels are cross-linked polymer networks that can absorb large amounts of water without dissolving or loosing their shape. A number of hydrogels are stimuli-sensitive. They can change their structures and properties in …
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Biophysical characterization of the Escherichia coli RNA degradosome
… discrete particles of the enzyme complex on a supported lipid bilayer on glass. To enable in situ studies of the degradosome, the HaloTag was encoded at the end of the rne gene within the E. coli chromosome using CRISPR/Cas. In order to extract the labeled degradosome from its native lipid …
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Differential Interactions of Lipopolysaccharides with Lipid Bilayers: Applications for Pathogen Detection
… presentation of LPS by inserting the hydrophobic lipid A portion of the molecule into a lipid bilayer, leaving the O antigen exposed. This method is advantageous because it minimizes exposure of the highly conserved lipid A epitopes, and maximizes exposure of the serogroup specific O antigens. In …
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Identifying the Role of Phospholipase D1 and Phosphatidic Acid in Exocytosis
… significant changes in membrane curvature, and lipids and curvature-sensing proteins can assist these processes. One protein in particular, phospholipase D1 (PLD1), and its product, the lipid phosphatidic acid (PA), are involved in multiple exocytic processes. However, PLD1 and PA’s role in this …
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DROSOPHILA CLASS-I MYOSINS THAT CAN IMPACT LEFT-RIGHT ASYMMETRY HAVE DISTINCT ATPASE KINETICS
… bias via in vitro gliding assays on a supported lipid bilayer. Despite myo1C’s lack of directionality at a protein level, there must be an intrinsic property in its motor domain to promote directionality at cellular to organismal levels. We performed steady-state and transient kinetics …
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Identifying the Relevance of C-Reactive Protein Conformers and the Advancement of a Membrane Curvature Binding Assay
<p>Lipid membranes play a vital role in cell signaling processes. Membrane shape and lipid content affect interactions between cellular membranes and proteins. This research focuses on characterizing those interactions and their impact by using various biochemical and biophysical assays. These …
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The stability of phospholipid and biomimetic polymer vesicles
… have demonstrated similar properties as phospholipids and incorporate AqpZ. Previous research conducted on AqpZ:ABA vesicles showed permeabilities exceeding commercial RO membranes and high selectivity. These properties make biomimetic materials promising for RO, but investigation of their …
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Observing, Understanding, and Manipulating Biological Membranes
<p>The phospholipid bilayer is one of the hallmarks of eukaryotic life. This complicated two dimensionally fluid surface is composed of a double layer of lipids which have a region that is hydrophobic and a region that is hydrophilic. The lipid bilayer membranes of a cell act as a barrier that …
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Development of novel bio/nano interfaces for materials science and biomedical applications
… developed a porous silica nanoparticle-supported lipid bilayer (protocell') for targeted delivery applications. Protocells combine enhanced specificity, stability, and cargo capacity when compared to similarly-sized liposomes. They can, additionally encapsulate and deliver multicomponent …
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THE NON-ERYTHROID MEMBRANE SKELETON AND ITS ROLE IN MECHANOTRANSDUCTION
… cellular behavior of 3T3 fibroblasts on glass supported lipid bilayers depend on the detail of lipid charge mixtures in the bilayer as well as the head group compositions. Live cell-supported lipid membrane hybrids are frequently used in dissecting membrane based inter-cellular communication. …
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Physical Encapsulation of Interface Bilayers
… material system which features interface lipid bilayers capable of hosting a wide variety of natural and engineered proteins. This research builds on the droplet interface bilayer (DIB) platform which first demonstrated that, through self-assembly, lipid-encased water droplets submersed in …
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Monitoring Biological Events on Native Cell Membranes with Organic Bioelectronics
… system, involving cell-derived supported lipid bilayers (SLBs) integrated with bioelectronic devices, is able to rapidly sense drug responses from cell membrane. This system eliminates the complexities and challenges of traditional testing systems, such as sterile cell-culture …
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Development of Bioelectronic Technologies for the Investigation of Extracellular Vesicle Function and Anti-Cancer Drug Discovery
… vesicles (EVs) are cell-secreted phospholipid bilayer-delimited particles of varying size and composition that facilitate intercellular communication. EVs play a critical role in a wide range of diseases by mediating the transfer of active biomolecules between cells, both in the vicinity …
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Multivalent Programmable Interactions Between Lipid Vesicles: Towards Responsive Soft Materials
Lipid membranes and lipid vesicles have been studied extensively in the last 50 years in order to characterise their biological, chemical and physical properties. Such work is of interest from a fundamental biological perspective, but also due to the applications that their biocompatibility …
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Model Membranes Study the Lipid-Reactivity of HIV-1 Antibodies and Vaccine Antigen
… 4E10 are required to first associate with HIV-1 lipids before binding to the target MPER antigen, however, little is known about how lipid membranes contribute to NAb-antigen binding. To this end we have developed model membrane systems to study NAb and antigen lipid interactions. </p><p>We first …
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