Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Superfusion"”.
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Utilisation de la technique de superfusion en cascade pour le dosage de l'activité réninique du plasma
… pour les essais biologiques dans des bains de superfusion en cascade mis au point par Gaddum (1953) et avons fait les observations suivantes: 1. La contraction du côlon de rat peut être rendue spécifique à l'angiotensine en infusant avec la solution de superfusion une mixture d'inhibiteurs …
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Prolactin Effects on Nigrostriatal and Mesolimbic Dopaminergic Neurons: In Vivo and In Vitro Studies
… both in vivo push-pull perfusion and in vitro superfusion techniques coupled to HPLC assay, the effect of prolactin on nigrostriatal and mesolimbic dopaminergic neuronal activity and its possible regulatory mechanism were examined.
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The electrophysiological effects of Endothelin-1 in human atrial myocytes
… by ET-1 at 10 nM (P< 0.05, n=15 cells). Superfusion with ET-1 (10 nM) alone had no significant effect on APD50, APD90, nor ERP (n=21 cells). There were no significant interactions between these electrophysiological effects and diseases states or chronic pre-operative drug therapy. …
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The effects of the tubulin-binding drug, colchicine, on the electrophysiological aspects of phototransduction in toad rod photoreceptors
… of cytoskeletal antagonists. Colchicine superfusion onto intact retinas hyperpolarized rods, increased their membrane resistance and altered the kinetics of voltage responses to light flashes. These effects persisted during blockade of rod inner segment conductances, but colchicine's …
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Characterization of Luteinizing Hormone - Releasing Hormone (Lhrh) Release From Rat Hypothalamus Using an in Vitro System
… rat hypothalamus was examined using an in vitro superfusion system and sensitive assays for LHRH measurement. High extracellular (60 mM) K('+) ion repeatedly stimulated in vitro LHRH release, an effect attributed to nerve membrane depolarization triggering influx of Na('+) and Ca('++) ions and …
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Characterization of neural ion regulation dysfunction during insult and evaluation of micro-opioid receptor activation during simulated ischemia in the pre-botzinger complex
… analyze the system. To attenuate this effect, a superfusion system was designed and built to provide fresh extracellular solution to the tissue. Furthermore, dielectric spectroscopy was utilized to analyze the change in conductivity of the extracellular solution as a result of various simulated …
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Freisetzung von zytoplasmatischem GABA ([3H]-GABA) und Glutamat (D-[3H]-Aspartat) durch Transporterumkehr (Heteroexchange) im Striatum von Ratte und Kaninchen
… ist nicht auszuschliessen, dass die unter der Superfusion mit Gabapentin beobachtete Abnahme der spontanen Freisetzung von Glutamat und Aspartat auf das Reaktionsprodukt der Lactamisierung, Gabapentin-Lactam, zurückzuführen ist.
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Progesterone Action on Hypothalamic Lhrh Neurons: In Vitro and In Vivo Studies
… (LHRH) neurons, as estimated by in vitro (superfusion) and in vivo (push-pull perfusion) techniques. The administration of P unequivocally stimulated the release of LHRH from hypothalamic tissue obtained from ovariectomized, estradiol-primed (OVX + E) rats as well as proestrous rats. The …
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A Role for glutamate and Gaba in attention-deficit/hyperactivity disorder: a study of the spontaneously hypertensive rat
… were investigated, using an in vitro superfusion technique. Since early life stress has been shown to increase the risk of developing ADHD, the present thesis also investigated how a model of early life stress (maternal separation) affects behavioural responses to novelty (behavioural …
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Mechanisms of ion channel activation in primary cultured and clonal cell lines
… approximately 90 s after onset of arachidonate superfusion. Arachidonic acid also induced kinetic modifications of the current which were evident as an acceleration of the time-dependent· inactivation exhibited at depolarised potentials. Inhibitors of cyclo-oxygenases, lipoxygenases and …
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Beeinflußung der [³H]-Acetylcholin-Freisetzung im Rattenhippocampus und menschlichen Neokortex durch ß-Amyloid 1-28 und durch Hemmung der Cholinacetyltransferase
Im ersten Teil der vorliegenden Arbeit wurde im Superfusionsexperiment die Wirkung des aus 28 Aminosäuren bestehenden Amyloid-ß-Proteins (Aß1-28) auf die [³H]-Acetylcholin (ACh)-Freisetzung und [³H]-Cholin-Beladung im menschlichen Neokortex und Rattenhippocampus untersucht. Aß1-28 ist ein …
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The effect of light on a rat model of depression
… [3H]DA release in the NAc using in vitro superfusion. Serotonin levels in the hypothalamus and PFC were determined using Enzyme-Linked ImmunoSorbent Assay (ELISA). Western blot analysis was used to measure MOR-1 levels in the NAc, OXR-1 and OXR-2 in the PFC. Results: MS caused a …
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Locus-coeruleus norepinephrine system function in a developmental animal model of schizophrenia: the socially isolated rat
… one of two neurochemical analyses. For in-vitro superfusion experiments the tissue was used immediately to quantify functional release of radioactively-labelled norepinephrine when stimulated with glutamate under varying conditions. Enzyme linked immunosorbent assays (ELISA) and bicinchoninic …
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Pharmacological Characterization of Prejunctional Serotonin Receptors in Mammalian Anterior Uvea
… [3H]-5-HT and [3H]NE release using the superfusion method.|Electrical field stimulation of iris-ciliary bodies preloaded with [3H]-5-HT caused an increase in tritium efflux over baseline. Since chromatographic separation of metabolized and unmetabolized [3H]-5-HT released from isolated …
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Eine Methode zur Messung der neuronalen Glutamatfreisetzung
Vorrangiges Ziel dieser Arbeit war es, eine Methode zur Abtrennung neuronal freigesetzten Glutamats zu etablieren. Glutamat spielt als wichtigster erregender Transmitter eine zentrale Rolle in Funktion und Erkrankung des ZNS. Eine Untersuchung der pharmakologischen Modulation der …
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Cellular mechanism of contractile dysfunction in diabetes-induced cardiomyopathy
… compared to age-matched control, respectively. Superfusion of myocytes with HT (high osmolarity) caused alterations in both the amplitude and time course of shortening in myocytes from STZ-treated and age-matched control rats. Resting cell length was significantly decreased on exposure to HT and …
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Modulation der neokortikalen Neurotransmission durch exogene und endogene Cannabinoide unter Berücksichtigung möglicher Speziesunterschiede zwischen Mensch und Tier
… gemessenen Bindungs-Ki von AEA lagen. Analog den Superfusionsergebnissen kann also eine größere (patho)-physiologische Bedeutung von ECs im Menschen vermutet werden. CB1-R-unabhängig hemmten Arachidonsäure, AEA und WIN55212-2 den [³H]-5-HT- und [³H]-DA-Uptake im Neokortex der Ratte. Mittels eines …
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Inhibition of excitatory neurotransmitter activity by hydrogen sulfide in bovine retina, in vitro
… in bovine isolated retina using the superfusion method. Furthermore, I investigated the ability of various H2S donor drugs to protect bovine retinal neurons from glutamate-induced toxicity using the MTT assay. |The H2S donor drugs, L-cysteine (100 nM to 10 µM), NAC (10 µM to 1 mM) and …
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Präsynaptische Modulation der Freisetzung von Acetylcholin : Untersuchungen im Neocortex des Menschen sowie an cholinergen Neuronenkulturen aus der Septumregion des fetalen Rattenhirns
Die vorliegende Arbeit untersucht u.a. anhand von elektrisch evozierter Freisetzung tritiierter Substanzen aus Neocortexgewebeschnitten des Menschen sowie neuronaler in vitro Zellkulturen des fetalen Rattenseptums den modulatorischen Einfluss verschiedener neuronaler Rezeptoren auf die Freisetzung …
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The role of norepinephrine in arousal and attention: a human and rat study
… of NE from LC-NE terminals was achieved by a superfusion technique. The 'hyporaroused' rats (SHR) showed exaggerated release of NE from LC-NE hippocampal terminals when stimulated with glutamate as compared to the 'hyperaroused' rats (WKY). Glutamate ionotropic receptor antagonism yielded …