Global ETD Search
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Showing 1 to 12 of 12 for “"Supercomplexes"”.
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Protein Factors Regulating Mitochondrial Respiratory Supercomplexes
… organize into supramolecular structures called supercomplexes (ETC SCs). Protein factors regulating ETC SCs remain largely unknown despite their fundamental implications to mitochondrial respiratory function. Recent knock-out studies have delineated external ETC proteins HIGD1A and HIGD2A as …
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Hibernation is super complex: dynamics of electron transport system supercomplexes
The electron transport system complexes form supercomplexes (SCs) within mitochondrial membranes, perhaps increasing respiratory capacity or reducing reactive oxygen species production. My project aimed to determine the abundance, composition, and stability of SCs in a hibernator. Hibernators have …
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Identification and functional characterisation of different inner mitochondrial membrane supercomplexes
… associate in higher-order assemblies, known as supercomplexes (SC), that are structurally interdependent. Numerous patients carrying mutations in a single complex, indeed, present with combined enzyme deficiencies and, in particular, the destabilisation of complex I (CI) has been often described …
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Electron cryomicroscopy structures of respiratory supercomplexes from alphaproteobacteria suggest mechanisms to enhance catalysis and to prevent deactivation of respiratory complex I
… form oligomeric membrane assemblies known as supercomplexes. Even though they are found throughout nature and several physiological consequences are associated with their depletion, it is not clear why they have evolved and what their selective advantage is. While the role of mitochondrial …
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Effect of experimental complex III deficiency on respiratory chain assembly and function
… thesis were to investigate CIII assembly and supercomplexes in a mouse model harboring the Bcs1l mutation c.232A>G. In homozygotes, the mutation results in a progressive CIII deficiency mimicking the human syndrome. To elucidate the role of the RISP subunit, wild type mice were exposed to CIII …
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Rydberg spectroscopy of calcium monofluoride
… are strong and they repeat in consecutive n*-supercomplexes; however, in the n* = 17 to 18, v⁺ = 1 autoionizing region, the core-penetrating states start to disappear due to predissociation and the non-penetrating states become the dominant spectroscopic feature.
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Structural Studies into the Mechanism and Organisation of Mammalian Respiratory Complex I
… information for complex I and its constituent supercomplexes from immortalised human cell lines remains limited despite its potential as a system for studying human complex I disease mutations and its unique metabolic adaptations. Chapter 2 uses structural data from two high-resolution cryo-EM …
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The Molecular Anatomy of Mitochondrial Disease: Identification and Characterisation of Novel Nuclear-Encoded Mitochondrial Disease Genes
… showed destabilisation of CI-containing supercomplexes. TMCO6 was found to co-localise with the CI holocomplex by 2D-BNGE, and this result was further corroborated by immunoprecipitation experiments. Additionally, cellular models for TMCO6 gene silencing and overexpression were …
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Mitochondrial Calcium Uptake: LETM1 and MICU1 Are Mitochondrial Proteins That Regulate Mitochondrial Calcium Homeostasis and Cellular Bioenergetics
… LETM1 has been implicated in formation of the supercomplexes of the electron transport chain (Tamai et. al., 2008). In support, these studies show that LETM1 knockdown results in increased reactive oxygen species (ROS) production. These results for the first time demonstrate that LETM1 controls …
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Functional analysis of plastid-encoded genes
… with PSII cores. In particular, PSII-LHCII supercomplexes could no longer be isolated from transplastomic tobacco plants. Furthermore, the minor chlorophyll a/b-binding proteins CP26, and to a lesser extent CP29, were substantially reduced under most growth conditions analyzed, in both, …
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Cryo-EM as a Tool to Study the Diverse Interactome of Ku 70/80 in NHEJ and Beyond
… multi-component assemblies, defined as NHEJ supercomplexes. Further attempts to obtain cryo-EM models of Ku with additional non-core NHEJ components were carried out, with some allowing the visualisation of high-resolution structures, such as that of Ku with the accessory factor Paralog of …
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Wpływ bisfosfonianów stosowanych w terapii osteoporozy na metabolizm tlenowy komórek śródbłonka
… and reorganization of respiratory chain supercomplexes. Increased ROS production is associated with higher reduction level of mtCoQ. It was observed for the first time that N-BPs can lead to decreased level of heme a in mitochondria. Therefore, these results indicate that N-BP-induced …