Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 69 for “"Super-resolution microscopy"”.
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Super-resolution Microscopy: Novel Developments and Optimisations
… of a multifunctional localisation based super-resolution microscope at Cambridge Advanced Imaging Centre. The microscope is optimised to perform single and dual-colour imaging with high localisation precision and accuracy. Moreover, three-dimensional imaging capability is included in the …
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Hardware, software, and applications of super-resolution microscopy
Modern microscopy techniques can image beyond the diffraction limit, in three spatial dimensions, and capture sub-cellular resolution videos, providing new biological insight and assisting in drug development. However, such advanced instruments typically require expert engineers and physicists to …
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Cellular responses to external threats probed by super-resolution microscopy
Fluorescence microscopy has been an essential tool in biology. However, its imaging resolution has been limited around >200 nm in lateral dimensions, and >500 nm in axial dimension, leaving many biological structures too small to study. However the recent developments of several super-resolution …
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Advances in volumetric super-resolution microscopy and single-particle tracking
Single-molecule localisation microscopy (SMLM) has enabled optical microscopy to probe biological structures and processes at the nanometer scale. To accommodate the inherently 3D nature of biological structures, 3D-SMLM techniques---such as the astigmatic and double-helix point spread functions …
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Deep learning for image processing in optical super-resolution microscopy
Optical microscopy is fundamentally governed by a trade-off between image quality, imaging speed and duration. The quality can be considered a function of the signal-to-noise ratio, contrast and image resolution, which are all limited by the amount of light that can be acquired within a set …
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QUANTIFYING OLIGOMERIC STATES OF PROTEINS IN-CELL VIA SINGLE-MOLECULE SUPER-RESOLUTION MICROSCOPY
… using protein localizations obtained from super-resolution microscopy. A single-molecule assay was developed to extract the surface protein oligomer populations using the probability density function of molecule density (PDFMD). We provided the theoretical model of PDFMD, further validated …
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Intracellular labeling of live cells via transient membrane permeabilization for fluorescence and super resolution microscopy
… motion of labeled actin and mitochondria using super-resolution fluorescence microscopy (dSTORM). Furthermore, we show the ability to image single proteins inside the living cell by tracking single molecules of kinesin using photostable probes.
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Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control
High resolution imaging in three dimension is important for biological research. The RESOLFT (Reversible Saturable Optical Fluorescence Transitions) fluorescent microscopy is one technique which can achieve lateral super-resolution imaging. Two-photon microscopy naturally generate high resolution …
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Single-molecule techniques for mapping protein aggregates
… are required to unravel their compositions. Super-resolution microscopy is particularly useful, as these aggregates are smaller than the diffraction limit of light. Compared to conventional light microscopy, the fluorescence of the nearby molecules in super-resolution microscopy fluoresces at …
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Quantitative mRNA detection with advanced nonlinear microscopy
… two nonlinear technologies, second harmonic super-resolution microscopy (SHaSM) and transient absorption microscopy (TAM), are developed to detect and quantify single Human edimer receptor 2 (Her2) mRNA transcripts. The SHaSM is used to detect single mRNA transcript beyond the diffraction …
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A general method for three color STED microscopy with one depletion laser : application to primary neuronal culture
… phenomena with unprecedented spatial resolution. Indeed, early studies employing these methodologies have revealed an extraordinary level of nanoscale spatiotemporal organization of complex and highly dynamic molecular architectures at the pre- and postsynaptic compartments. How …
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Adaptive optics stimulated emission depletion microscope for thick sample imaging
Over the past few decades, fluorescence microscopy has proven to become the most widely used imaging technique in the field of life sciences. Unfortunately, all classical optical microscopy techniques have one thing in common: their resolution is limited by the diffraction. Thankfully, due to the …
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Graphene Brain on a Chip Platform for the Study of Neurodegeneration
… calcium imaging, electrophysiology, and super-resolution imaging data obtained, and a physiologically relevant application for the graphene MEAs in neurodegeneration research. The fabricated devices demonstrated a higher spatiotemporal resolution and lower impedance than commercially …
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Toward Ultra-Resolution Biomolecular Mapping in Cells with Expansion Microscopy
… functions, achieving nanoscale spatial resolution in biomolecular imaging is essential. Expansion microscopy (ExM)1, a new kind of super-resolution microscopy, enables this by physically enlarging preserved biological specimens. Thus allows the investigation of structure-function …
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Design and Evaluation of Nanoparticle Systems for Bacterial Targeting
… interactions with bacterial envelopes using super-resolution microscopy. cFLs, lipid-based NPs engineered to fuse with cellular membranes, have been previously studied for their ability to deliver antimicrobial agents across the permeability barrier of the bacterial outer membrane (OM). In …
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Organization and Regulation of Proteins Required For Initiation of Cell Division In Escherichia Coli
… FtsA, and ZipA in normal cells using 3D-SIM super-resolution microscopy, which showed that they generally colocalize in puncta instead of a uniformly continuous ring. Finally, I investigated the assembly of purified FtsA and FtsA mutant proteins by electron microscopy and assessed their …
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The localisation and role of Sidekick at apical vertices in Drosophila epithelial morphogenesis
… of epithelial vertices, the Immunoglobulin-superfamily domain protein Sidekick. I have characterised the localisation of Sidekick tagged with YFP in a number of Drosophila epithelial tissues and found that in most epithelial tissues, Sidekick-YFP localises to vertices at the level of …
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Quantum simulation with an optical kagome lattice
… for active phase stabilization for bichromatic superlattices. We show that we have overcome these challenges and give a thorough account of our machine's technical details. Furthermore, we present calculations and measurements that fully characterise the kagome quantum simulator. Much of the …
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