Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Super-resolution fluorescence microscopy"”.
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Super-Resolution Fluorescence Microscopy using Transparent Waveguide Chips
Super-resolution fluorescence microscopy (optical nanoscopy) allows us to surpass the optical diffraction limit and observe biological phenomena at the nanoscale using visible light. Different modalities come with their own trade-offs, such as imaging speed vs. achieved resolution or live cell …
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Next-generation fluorophores for single-molecule and super-resolution fluorescence microscopy
The development of single-molecule and super-resolution fluorescence techniques has revolutionised biological imaging. Nano-scale cellular structures and heterogeneous dynamic processes are now able to be visualised with unprecedented resolution in both time and space. The achievable localisation …
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Studying synaptic organization and dynamics of AMPA receptors in primary neuronal cultures and brain tissue using super-resolution fluorescence microscopy
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2025-12-01
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Computational microscopy for sample analysis
Computational microscopy is an emerging technology which extends the capabilities of optical microscopy with the help of computation. One of the notable example is super resolution fluorescence microscopy which achieves sub-wavelength resolution. This thesis explores the novel application of …
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Intracellular labeling of live cells via transient membrane permeabilization for fluorescence and super resolution microscopy
Fluorescence imaging of intracellular proteins is often achieved by using transfection-induced expression of fluorescent protein. This can potentially impose artifacts such as loss of function or over-expression of target proteins. Direct labeling of the intracellular protein is an alternative to …
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Characterization of the Morphology, Composition, and Activity of the Germ Plasm in Nasonia vitripennis
… within the oosome. Using a combination of super-resolution fluorescence microscopy and transmission electron microscopy, I identified lipid droplets and ribosome-associated vesicles widely distributed throughout the oosome interior, where mitochondria are notably absent. I also identified …
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Cellular delivery and site-specific targeting of organic fluorophores for super-resolution imaging in living cells
Recent advances in super-resolution fluorescence microscopy have pushed the spatial resolution of biological imaging down to a few nanometers. The key element to the development of such imaging modality is synthetic organic fluorophores with suitable brightness and photostability. However, organic …
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Single-Objective Tilted Light Sheet Illumination with Exchange-PAINT and Deep Learning for Fast, Accurate, and Precise 3D Single-Molecule Super-Resolution Imaging in Mammalian Cells
Single-molecule super-resolution fluorescence microscopy is a powerful method for imaging detailed biological structures at the nanoscale. However, imaging capabilities in thick samples such as mammalian cells remain limited due to increased fluorescence background which degrades the achievable …
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Visualizing Mast Cell Activation: Single Molecule Dynamics of Early Events in FceRI Signaling
… In this work we employ single molecule fluorescence imaging techniques to quantify adapter protein recruitment, lateral mobility, receptor aggregation, and cytoskeletal organization to create a better understanding of many key processes in immune cell regulation. We focus on …
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Applying single-molecule localisation microscopy to achieve virtual optical sectioning and study T-cell activation
Single-molecule localisation microscopy (SMLM) allows imaging of fluorescently-tagged proteins in live cells with a precision well below that of the diffraction limit. As a single-molecule technique, it has also introduced a new quantitative approach to fluorescence microscopy. In the Part A of …
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Structural Organization and Chemical Activity Revealed by New Developments in Single-Molecule Fluorescence and Orientation Imaging
<p>Single-molecule (SM) fluorescence and its localization are important and versatile tools for understanding and quantifying dynamical nanoscale behavior of nanoparticles and biological systems. By actively controlling the concentration of fluorescent molecules and precisely localizing individual …
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Bilinear inverse problems with sparsity: Optimal identifiability conditions and efficient recovery
Bilinear inverse problems (BIPs), the resolution of two vectors given their image under a bilinear mapping, arise in many applications. Without further constraints, BIPs are usually ill-posed. In practice, parsimonious structures of natural signals (e.g., subspace or sparsity) are exploited. …