Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 58 for “"Super resolution imaging"”.
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Super-resolution Imaging of Chromatin and Functional Nuclear Architecture
… genes are poorly understood. Newly developing super-resolution imaging techniques offer an important new way to investigate structural features of the genome, however, imaging chromatin within the nucleus is traditionally very difficult due to the dense packaging with a small nuclear volume. …
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Super-resolution imaging of glutamate receptors trafficking at live synapses
… fluorescent microscopy with diffraction-limited resolution. As a result, the detail localization of AMPA diffusion sites on the synapse cannot be resolved. To address these problems, we employed high-precision three-dimensional single particle tracking (SPT) to image receptor diffusion; and …
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Using photo-instability to quantify fluorophores and achieve super-resolution imaging
… and quantifying fluorophores in biological imaging applications. Fluorophore photobleaching, blinking, binding, etc., is exploited to quantify and localize single fluorophores, even when their fluorescent images overlap those of nearby fluorophores. In the first technique, we image single, …
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Quantitative super-resolution imaging of cell polarity proteins using DNA-PAINT
… understanding their function. The development of super-resolution imaging has improved our ability to garner this information, but counting individual molecules in densely-packed assemblies is still challenging. DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT) is one of …
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Investigating the role of protein aggregation in neurodegenerative diseases using super-resolution imaging
… idealised test tube conditions. In this work, super-resolution fluorescence imaging is firstly applied for the study of alpha-synuclein, the protein associated with Parkinson's disease. A new method is described combining single-molecule localisation microscopy and an alpha-synuclein aggregate …
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Advanced applications of Raman spectroscopy and super-resolution imaging of biological and plant materials
… topics are discussed: a developed multimodal imaging method to study gene function in plants, the potential for Raman spectroscopy to infer relative plant metabolite content for optimal harvest, and the prospects of using inorganic semiconductor quantum dots with saturated excitation …
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Development of Three-Dimensional Super-Resolution Imaging Using a Double-Helix Point Spread Function
… limited by spherical aberration present when imaging away from coverslip surfaces to the study of small volumes close to the coverslip. By matching the refractive index of the objective lens immersion liquid to that of the imaging media, this aberration can be minimised, facilitating …
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Development of optical systems and imaging analyses for hyperspectral microscopy and 3D super-resolution imaging
… few decades to address the demands of higher resolutions (both spatial and temporal) as well as multicolor imaging in order to further the efforts of biological and medical science. Super-resolution microscopy techniques are capable of achieving resolutions several factors greater than …
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Super-resolution imaging of glutamate receptors in cultured hippocampal neurons under basal and LTP conditions
… and structure of AMPARs. With three-dimensional super-resolution microscopy (7~16 nm resolution in x-y and 20~32 nm resolution in z), we study AMPARs and NMDARs under basal and cLTP conditions. Under basal condition, we compare two different size fluorophores attached to AMPAR or NMDAR: one is …
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Cellular delivery and site-specific targeting of organic fluorophores for super-resolution imaging in living cells
Recent advances in super-resolution fluorescence microscopy have pushed the spatial resolution of biological imaging down to a few nanometers. The key element to the development of such imaging modality is synthetic organic fluorophores with suitable brightness and photostability. However, organic …
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Single-Objective Tilted Light Sheet Illumination with Exchange-PAINT and Deep Learning for Fast, Accurate, and Precise 3D Single-Molecule Super-Resolution Imaging in Mammalian Cells
Single-molecule super-resolution fluorescence microscopy is a powerful method for imaging detailed biological structures at the nanoscale. However, imaging capabilities in thick samples such as mammalian cells remain limited due to increased fluorescence background which degrades the achievable …
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Visualizing Mast Cell Activation: Single Molecule Dynamics of Early Events in FceRI Signaling
… this work we employ single molecule fluorescence imaging techniques to quantify adapter protein recruitment, lateral mobility, receptor aggregation, and cytoskeletal organization to create a better understanding of many key processes in immune cell regulation. We focus on understanding the …
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Adaptive optics stimulated emission depletion microscope for thick sample imaging
… has proven to become the most widely used imaging technique in the field of life sciences. Unfortunately, all classical optical microscopy techniques have one thing in common: their resolution is limited by the diffraction. Thankfully, due to the very strong interest, development of …
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Coherent control of nitrogen-vacancy centers in diamond nanostructures for quantum sensing and networking
… have led to numerous applications ranging from super-resolution imaging to the exploration of previously untested new phenomena using quantum entanglement for information processing and sensing. The solid-state environment of the diamond allows us to engineer nanostructures, which are promising …
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Arbitrarily-controllable programmable aperture light field cameras : design theory, and applications to image deconvolution & 3-dimensional scanning
… Deconvolution," as well as to 3D scanning and super-resolution imaging. The use & performance of ACPA cameras in these applications is explored both in theory and with experimental results. Additionally, a framework for ACPA camera design for optimal 3D scanning is described.
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Trans-skull Ultrasound for Imaging and Therapy
… as a novel modality for the treatment and imaging of brain diseases. When enhanced by circulating microbubble agents, which scatter sound and vibrate in response to the incident ultrasound, it can enable a range of new therapeutic interventions and open new possibilities for imaging. …
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Parallel and flexible fluorescent imaging using two-photon RESOLFT super-resolution microscopy with spatial light modulator control
High resolution imaging in three dimension is important for biological research. The RESOLFT (Reversible Saturable Optical Fluorescence Transitions) fluorescent microscopy is one technique which can achieve lateral super-resolution imaging. Two-photon microscopy naturally generate high resolution …
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Super-resolution phased array sonar imaging
This dissertation applies super-resolution signal processing alogrithms to image reconstruction for a multibeam phased array sonar. A generic data model for phased array sonars is first developed and is used to derive conventional signal processing concepts for image reconstruction which are …
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Nanolaminate coatings to improve long-term stability of plasmonic structures in physiological environments
… research endeavors. They are used in sensing, super-resolution imaging, SPP lithography, SPP assisted absorption, SPP-based antennas, light manipulation, etc. To take full advantage of the attractive capabilities of CMOS compatible low-cost plasmonic structures based on Al and Cu, nanolaminate …
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EVALUATING THE USE OF TURBIDIMETRY FOR STUDYING FIBRIN STRUCTURAL PROPERTIES
… to those obtained experimentally using SEM and super-resolution imaging. The summation of this work will provide a framework for utilizing turbidimetry to study fibrin and pave a path for utilizing turbidimetry in a clinical setting.
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