Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"Sulfotransferase"”.
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ROLES OF SULFOTRANSFERASE ENZYMES IN TRANS-PLACENTAL DISPOSITION
… transfer. Several isoforms of the sulfotransferase enzymes are functional in placenta but there is only limited information available on the utility of cultured trophoblast cells for studying placental sulfation. We examined the expression and activities of four sulfotransferase …
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Sulfotransferase-vermittelte Genotoxizität von benzylischen Metaboliten alkylierter polyzyklischer aromatischer Kohlenwasserstoffe
… und nachfolgende Sulfonierung katalysiert über Sulfotransferasen (SULT) zu reaktiven Schwefelsäureestern umgesetzt werden. Die SULT-vermittelte Bioaktivierung zu einem genotoxischen Schwefelsäureester wurde für den benzylischen Alkohol 1-Hydroxymethylpyren des Hepatokanzerogens 1-Methylpyren in …
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Tyrosine Sulfation in A431 Cells
… identified and characterized a unique tyrosine sulfotransferase and its substrate in A431 cells. I also established an in vitro assay method for tyrosine sulfotransferase activity, which opened a door towards enzyme purification Sulfation of proteins on tyrosine residues has recently been shown …
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Estrogen sulfotransferase (sult1e1) expression and function in mcf10a-series breast epithelial cells: role as a modifier of breast carcinogenesis and regulation by proliferation state
<p>Estrogen sulfotransferase (SULT1E1) catalyzes the sulfonation of estrogens, which limits estrogen mitogenicity. TaqMan Gene Expression assays were used to profile the mRNA expression of estrogen receptor (ERα and ERβ) and estrogen metabolism enzymes including cytosolic sulfotransferases …
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In vitro characterization of aromatase, estrone sulfotransferase and estrone sulfatase activities in the porcine placenta and endometrium at 30, 60 and 90 days of gestation
… of three enzymes, aromatase, estrone (E1) sulfotransferase and E] sulfatase, in the porcine placenta and endometrium on d 30, 60 and 90 of gestation. These enzymes play key roles in determining in utero concentrations of estrogens. Days 30, 60 and 90 were chosen because previous …
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Heparan sulfate glycosaminoglycan regulation of vasculogenesis
… of HSGAG modifying enzyme, N-deacetylase/N-sulfotransferase-1, translated to inhibition of HSGAG sulfation and resulted in impaired blood vessel development in zebrafish embryos. Interestingly, vessel formation in both embryonic stem cell and zebrafish models was restored by the addition of …
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Molecular and biochemical characterization of hydroxyjasmonate and flavonoid sulfotransferases from Arabidopsis thaliana
… the biological function of all the sulfotransferase (ST) coding genes of A. thaliana . Based on amino acid sequence alignment with previously characterized soluble STs, we have identified 17 genes coding for putative STs in the genome of this plant. Prior to this work, only one …
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Wirkung von Etomidat auf zelluläre Regulationsmechanismen der Nebennierenrinde
… Wirkung auf die Aktivität der DHEA-Sulfotransferase. Auf mRNA-Ebene führte Etomidat zu einer leichtgradigen Verminderung der Expression von P450c17 und P450c21, auf Proteinebene ließ sich dahingegen eine deutlich vermehrte Expression von P450scc und eine leichtgradig vermehrte …
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Cartilage mechanobiology : the effects of loading on the fine structure and function of chondroitin sulfate glycosaminoglycans
… of interest: chondroitin sulfate and GalNAc 4S,6-sulfotransferase.
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Elucidation of gene clusters underlying withanolide biosynthesis in ashwagandha
… additional P450s (CYP88C7 and CYP88C10) and a sulfotransferase (SULF1) generate the characteristic A-ring structure of withanolides, featuring a C₁ ketone and C₂-C₃ unsaturation. The discovery of SULF1 as a core withanolide pathway enzyme challenges the conventional view of sulfotransferases as …
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Assessing Hepatic Gene Expression in Response to Xenobiotic Exposure in Mice
… (Gstm1; P = 0.049) on d 11 was increased, and sulfotransferase 5a1 on d 11 decreased (P = 0.02). Sulforaphane increased expression of cytochrome P450 1a2 on d 5 (P = 0.02) and flavin containing monooxygenases 1 on d 11 (P = 0.002), both phase I genes. It also increased expression of a phase II …
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Glycomics : an emerging paradigm to protein-carbohydrate interactions
… chondroitinase B, HSGAG 2-0 sulfatase and 3-0 sulfotransferase as model enzymes. For each of these enzymes a theoretical model for the enzyme-substrate structural complex is developed and it is coupled with the site directed mutagenesis and biochemical studies to determine its catalytic …
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The Role of Periplasmic Disulfide Bond Status in the Regulation of the Salmonella SPI1 Type Three Secretion System
… DsbI, downstream of a periplasmic arylsulfate sulfotransferase (ASST). Here we show that DsbL and DsbI function as a redox pair for disulfide bond formation and, as such, affect transcription of the SPI1 type three secretion system genes and activation of the RcsCDB system. In contrast to …
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Analysis and Engineering of Polyene Biosynthesis in Actinomycetes
… Gene deletion was used to identify the essential sulfotransferase involved. In Chapter 4, a novel genetic engineering approach dubbed "accelerated evolution" was explored with the clethramycin PKS, which had previously been used to generate multiple analogues of the polyketide rapamycin. In this …
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Charakterisierung von Sulfotransferasen im Gastrointestinaltrakt von Mensch und Ratte und Aktivierung von Promutagenen in V79-Zellen, die eine intestinale Form (1B1) des Menschen und der Ratte exprimieren
… Mukosa des Menschen und der Ratte mit Sulfotransferasen wurde mit Hilfe von Immunodetektion und Enzymaktivitätsmessungen untersucht. In Proben aus Colon und Rektum von 39 Personen wurden die Formen h1A1, h1A3 und h1B1 identifiziert, wobei in einer weiteren Probe, die als einzige von …
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Heparan Sulfate Proteoglycans in Pancreatic Ductal Adenocarcinoma (PDAC)
… from WT-KRAS mice. HS3ST1, a gene encoding for a sulfotransferase enzyme responsible for the synthesis of Tetra-1, is significantly upregulated in PDAC tumors and dynamically altered upon KRAS* inhibition. Loss of HS3ST1 in PDAC cell lines hamper proliferation as well as colony formation, and …
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FROM GENE TO STRUCTURE: Formation of Iduronic Acid in Dermatan Sulfate by Two DS-epimerases
… are simultaneously needed together with the sulfotransferase D4ST-1 to generate these types of domains. Interestingly, 4-O-sulfated iduronic acid blocks constitute the binding site for several growth factors, including fibroblast growth factor 2. Through this binding, dermatan sulfate can …
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Investigation of the role of VML in the establishment of Drosophila embryonic dorsal-ventral polarity
… the developing oocyte. pipe encodes a sulfotransferase that transfers sulfate groups to several protein components of the vitelline membrane layer of the eggshell, including Vitelline Membrane-Like protein (VML). These sulfated proteins represent a ventral cue embedded in the eggshell, …
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