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Showing 1 to 12 of 12 for “"Sulfolobus solfataricus"”.
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A Thermostable Cytochrome P450 From Sulfolobus Solfataricus
"Crystallographic studies have revealed the placement of conserved key catalytic residues and solvent in the active site that are important in the P450 dioxygen scission reaction. CYP119 is found to display a mobile F-G helix and loop region that undergoes a significant conformational change upon …
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Protein O-Kinases in the Archaeon Sulfolobus solfataricus
… The extreme acidothermophilic archaeon, <i>Sulfolobus solfataricus</i>, exhibits a membrane-associated protein kinase activity. Solubilization of the kinase activity requires the presence of detergent such as Triton X-100 or octyl glucoside, indicating its activity reside in an integral …
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Charakterisierung thermophiler Cellulasen aus Sulfolobus solfataricus und Thermotoga maritima
… SSO1949 aus dem hyperthermophilen Archaeon S. solfataricus P2 ist aufgrund ihrer extremen thermo- und acidophilen Eigenschaften interessant für industrielle Anwendungen. 2005 wurde das 37 kDa grosse Enzym biochemisch charakterisiert und weist ein pH- und Temperaturoptimum von ca. 1.8 und ca. …
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Identification of a protein kinase substrate in Sulfolobus solfataricus P2
… the study of the acidothermophilic organism Sulfolobus solfataricus. Such findings may provide answers into evolutionary relationships and facilitate an understanding of phosphate transfer via proteins in more elaborate systems where pathway disturbances can lead to disease processes. A …
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Characterization of SsoSSB, Sso1450, Sso2001 Proteins and Analysis of CRISPR and cas Genes from Sulfolobus solfataricus P2
… the complete sequencing of the genome of Sulfolobus solfataricus (Sso) P2, this organism has been widely used as a model strain for crenarchaea. The present work concentrated on the characterization of a newly discovered single-stranded binding (SSB) protein from Sso P2 and the …
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Purification and characterization of a protein phosphatase (PP1-Arch) from the archaebacterium Sulfolobus solfataricus, isolation and expression of its gene
… Soluble fraction was prepared from <i>Sulfolobus solfataricus</i>, from which PP1-Arch was purified over 1OOO-fold by DE-52 ion-exchange, hydroxyapatite, gel filtration (G- 100), and Mono Q FPLC chromatography. PP1-Arch was identified from the final purified sample by renaturation on an …
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Präparation und röntgenkristallographische Untersuchungen an archaebakteriellen Box C/D sRNPs und einer neuartigen Glukosyltransferase aus Thermotoga maritima MSB8
… den Proteinen aL7, aNop56 und aFibrillarin aus Sulfolobus solfataricus konnte rekonstituiert werden. Die Kristallisation des rekonstituierten Komplexes lieferte jedoch nur Kristalle, die aufgrund ihrer geringen Auflösung (~ 14 Å) nicht für eine Strukturaufklärung verwertbar waren. Eine Analyse …
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The "atypical" protein kinase, SsoPK5, an archaeal member of the piD261/Bud32 subfamily
… reading frame (ORF) sso0433 from the archaeon Sulfolobus solfataricus encodes a protein kinase, SsoPK5 that exhibits 33% sequence identity to p53 related protein kinase (PRPK) from Homo sapiens and 26% sequence identity to piD261/Bud32 from Saccharomyces cerevisiae. Given this high degree of …
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Protein Phosphorylation in Archaea
… Previously, in the sulfur-dependent archaeon Sulfolobus solfataricus a gene was cloned encoding a protein-serine/threonine phosphatase that was similar to eucaryal protein-serine/threonine phosphatases type 1, 2A, and 2B. To identify protein phosphatases in other archaeons, oligonucleotides …
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Isolation and characterization of new pterins from nonmethanogenic archaebacteria
… positively charged pterin has been isolated from Sulfolobus solfataricus. This pterin is very special since no positively charged unconjugated pterin had ever been found in nature before. This pterin is named solfapterin after the species name of the bacteria from which it was obtained. The …
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Dual-specific protein phosphatases in the <i>Archaea</i>
… <i>Pyrococcus horikoshii</i>, and <i>S. solfataricus</i>. Only one has been partially characterized, <i>Tk</i>-PTP from <i>T. kodakaraensis</i>. Hence, our current body of knowledge concerning the functional properties and physiological roles of these enzymes remains fragmented. The …
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Entwicklung eines Sulfolobus-E. coli Shuttle-Vektors basierend auf dem Plasmid pRN1
Sulfolobus spp., ein Vertreter der thermoacidophilen Crenarchaeota, ist auf dem Weg sich als wichtiger archaealer Modellorganismus zu etablieren. Aufgrund seiner Wachstumsbedingungen von 75-80 °C bei pH 3-3,5 ist Sulfolobus biotechnologisch ebenfalls von hoher Bedeutung, da seine Enzyme …