Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"Sulfolobus acidocaldarius"”.
-
Biosynthesis of Caldariellaquinone in Sulfolobus acidocaldarius
… of caldariellaquinone (CQ) has been studied in Sulfolobus acidocaldarius using a variety of methods. By growing cells with a series of tyrosines labeled with deuterium or ¹³C and measuring the extent and position at which label was incorporated into the CQ by mass spectrometry, it was concluded …
-
The mechanism of cyclic proteasome-mediated protein degradation in Sulfolobus acidocaldarius
Sulfolobus acidocaldarius, a member of the TACK archaea superphylum, is one of the closest, experimentally tractable Prokaryotic relatives of Eukaryotes. Importantly, like Eukaryotes, Sulfolobus cells maintain an ordered cell cycle, despite lacking eukaryotic-like cell cycle regulators (homologues …
-
Structural Characterization of the Transfer Ribonucleic Acids From Halobacterium Volcanii and Other Archaebacteria
… barkeri, Thermoplasma acidophilum, and Sulfolobus acidocaldarius), as analyzed by two-dimensional thin layer chromatography of the ('32)P-labelled nucleotides. Second, we describe the sequences of seventeen H. volcanii tRNAs (tentative for two) as determined by the oligonucleotide …
-
Investigations on the taxonomy and the genetics of caldoactive bacteria
… A UV inducible temperate phage was isolated from Sulfolobus acidocaldarius strain B-12. It was purified and partially characterised and a method of growing Sulfolobus on solid medium obtained.
-
DNA double-strand break repair studied by atomic force microscopy
… complex from the thermophilic crenarchaeote Sulfolobus acidocaldarius. The structural analysis identified four distinct architectural arrangements and demonstrates the key role of the Rad50 zinc hooks in the oligomerisation of the complex. AFM imaging showed a dynamic and Velcro-like …
-
Strategies for thiazole/oxazole-modified microcin discovery
… TOMM natural product from the archaeal species Sulfolobus acidocaldarius. I demonstrate that although genetic manipulation has been previously used for the identification of natural products, comparative metabolomics is difficult to use for routine identification of low-abundance natural …
-
Entwicklung eines Sulfolobus-E. coli Shuttle-Vektors basierend auf dem Plasmid pRN1
Sulfolobus spp., ein Vertreter der thermoacidophilen Crenarchaeota, ist auf dem Weg sich als wichtiger archaealer Modellorganismus zu etablieren. Aufgrund seiner Wachstumsbedingungen von 75-80 °C bei pH 3-3,5 ist Sulfolobus biotechnologisch ebenfalls von hoher Bedeutung, da seine Enzyme …
-
Charakterisierung thermophiler Cellulasen aus Sulfolobus solfataricus und Thermotoga maritima
… exprimiert wird, nämlich in dem Crenarchaeot Sulfolobus. Seit 2007 steht für Sulfolobus acidocaldarius ein Shuttle-Vektor-System zur Verfügung, in welchem SSO1949 unter Kontrolle verschiedener induzierbarer und konstitutiver Promotoren exprimiert wurde. Darüberhinaus wurde SSO1949 zur …
-
Die außergewöhnliche Diversität der der Proteolipide in Archaea: Multimere und monomere Rotoren mit sechs bis dreizehn Ionenbindestellen in A1AO-ATPasen
… (Motiv II in Helix zwei). 9. Aus Membranen von Sulfolobus acidocaldarius und M. jannaschii wurden durch Chloroform/Methanol Lipide extrahiert, anschließend wurde aus diesen Lipiden Liposomen hergestellt, in die die A1AO-ATPase aus M. jannaschii rekonstituiert wurde. Die Synthese von ATP konnte …
-
Biochemical and structural characterization of radical SAM enzymes Elp3 and viperin
… Methanococcus maripaludis (Euryarchaeota) and Sulfolobus acidocaldarius (Crenarchaeota). Neither cm5U nor mcm5U/ncm5U (modification in eukarya) was detected in the archaea; however, two new substances (549 Da and 454 Da) were detected. Further analysis is required to determine if these …