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Showing 1 to 20 of 44 for “"Sulfolobus"”.
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Biosynthesis of Caldariellaquinone in Sulfolobus acidocaldarius
… of caldariellaquinone (CQ) has been studied in Sulfolobus acidocaldarius using a variety of methods. By growing cells with a series of tyrosines labeled with deuterium or ¹³C and measuring the extent and position at which label was incorporated into the CQ by mass spectrometry, it was concluded …
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Charakterisierung des Replikationsenzyms ORF904 aus Sulfolobus islandicus
ORF904 ist ein multifunktionelles Enzym, welches durch das kryptische Plasmid pRN1 codiert wird. Das Protein besitzt eine C-terminale Primase/Polymerasedomäne und eine N-terminale ATPase/Helikasedomäne. Im Rahmen dieser Arbeit wurde die molekulare Funktion der N-terminalen Domäne genauer …
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A Thermostable Cytochrome P450 From Sulfolobus Solfataricus
"Crystallographic studies have revealed the placement of conserved key catalytic residues and solvent in the active site that are important in the P450 dioxygen scission reaction. CYP119 is found to display a mobile F-G helix and loop region that undergoes a significant conformational change upon …
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Mechanistic interactions between Sulfolobus islandicus and its viruses
… biology have been elucidated. The viruses of Sulfolobus are diverse in morphology, genomic content, and infection mechanisms. However, compared to bacterial and eukaryotic virus-host systems, little is known about these viruses or their interactions with their host. Utilizing Sulfolobus …
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Protein O-Kinases in the Archaeon Sulfolobus solfataricus
… The extreme acidothermophilic archaeon, <i>Sulfolobus solfataricus</i>, exhibits a membrane-associated protein kinase activity. Solubilization of the kinase activity requires the presence of detergent such as Triton X-100 or octyl glucoside, indicating its activity reside in an integral …
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Charakterisierung thermophiler Cellulasen aus Sulfolobus solfataricus und Thermotoga maritima
… exprimiert wird, nämlich in dem Crenarchaeot Sulfolobus. Seit 2007 steht für Sulfolobus acidocaldarius ein Shuttle-Vektor-System zur Verfügung, in welchem SSO1949 unter Kontrolle verschiedener induzierbarer und konstitutiver Promotoren exprimiert wurde. Darüberhinaus wurde SSO1949 zur …
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Evolutionary and ecological consequences of chronic infection in Sulfolobus islandicus
… I utilize the model system of the archaeal host Sulfolobus islandicus and its viruses, the Sulfolobus Spindle Shaped Viruses (SSVs), to better understand how chronic infection shapes these microbial populations. I show how chronic infection with SSVs gives the host a competitive benefit against …
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Identification of a protein kinase substrate in Sulfolobus solfataricus P2
… the study of the acidothermophilic organism Sulfolobus solfataricus. Such findings may provide answers into evolutionary relationships and facilitate an understanding of phosphate transfer via proteins in more elaborate systems where pathway disturbances can lead to disease processes. A …
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Estudio de Oxidación de Azufre Elemental con Sulfolobus Metallicus a 67ºc
… mecanismo de oxidación de azufre elemental con Sulfolobus metallicus a 67 ºC para la producción de ácido sulfúrico. Se realizaron tres experimentos. El experimento principal con el fin de estudiar la capacidad oxidativa de azufre elemental, por lo que se monitorearon variables como pH, acidez, …
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Population genomics of Sulfolobus islandicus: genome-wide recombination and antagonistic interactions
… entire microbial populations and communities. Sulfolobus islandicus is a model organism for studying microbial population biology due to its island population structure that isolates endemic populations to specific regions. A single population from the Mutnovsky Volcano region of Kamchatka, …
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Examining patterns of CRISPR immunity in natural populations of Sulfolobus islandicus
… between geographically isolated populations of Sulfolobus islandicus. S. islandicus is a thermoacidophilic crenarchaeon that has been isolated from acidic hot springs in the Northern Hemisphere. In this dissertation, a biogeographic pattern of isolation by distance, previously established for S. …
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Evolutionary studies through Sulfolobus islandicus genomics: Ancient relationships and modern dynamics
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2023-12-01
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The mechanism of cyclic proteasome-mediated protein degradation in Sulfolobus acidocaldarius
Sulfolobus acidocaldarius, a member of the TACK archaea superphylum, is one of the closest, experimentally tractable Prokaryotic relatives of Eukaryotes. Importantly, like Eukaryotes, Sulfolobus cells maintain an ordered cell cycle, despite lacking eukaryotic-like cell cycle regulators (homologues …
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Entwicklung eines Sulfolobus-E. coli Shuttle-Vektors basierend auf dem Plasmid pRN1
Sulfolobus spp., ein Vertreter der thermoacidophilen Crenarchaeota, ist auf dem Weg sich als wichtiger archaealer Modellorganismus zu etablieren. Aufgrund seiner Wachstumsbedingungen von 75-80 °C bei pH 3-3,5 ist Sulfolobus biotechnologisch ebenfalls von hoher Bedeutung, da seine Enzyme …
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Viral diversity and host-virus interactions in the model crenarchaeon Sulfolobus islandicus
… we find the hyperthermophilic crenarchaeon Sulfolobus islandicus which represents one of the few genetically tractable systems within the TACK (Thaumarchaeota, 'Aigarchaeota', Crenarchaeota and Korarchaeota) archaea. In this dissertation I focus on broadening our understanding of host-virus …
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Mechanisms and regulation of dsDNA break repair in the Sulfolobus genus of thermophilic archaea
… centre of all four rings. Here we reveal that in Sulfolobus species the inner catalytic chambers are made up of mixed beta rings composed of two subunits. The first plays a crucial structural role but appears catalytically inert, while the second conveys catalytic activity. Here we investigate an …
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The effect of particulate-induced hydrodynamic stress on the bioleaching of chalcopyrite by a Sulfolobus sp.
In slurry reactors, hydrodynamic stress originates primarily from interactions between particles and cells in the reactor. This form of stress has been shown to negatively affect the process performance. The present study centres on slurry reactors utilised in the bioleaching industry. The …
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An investigation into the effect of solid particulate phase on the bioleaching performance of Sulfolobus metallicus
… performance of the extreme thermophile Sulfolobus metallicus. The hypothesis of the thesis was as follows: Archae involved in bioleaching are susceptible to damage in agitated aerated vessels, especially with increasing pulp density.
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Characterization of SsoSSB, Sso1450, Sso2001 Proteins and Analysis of CRISPR and cas Genes from Sulfolobus solfataricus P2
… the complete sequencing of the genome of Sulfolobus solfataricus (Sso) P2, this organism has been widely used as a model strain for crenarchaea. The present work concentrated on the characterization of a newly discovered single-stranded binding (SSB) protein from Sso P2 and the …
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