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Showing 1 to 7 of 7 for “"Sulfhydryl Reagents"”.

  1. The emerging role of the a4(+)a4(-) interface as a determinant of functional signatures of a4ß2 nicotinic acetylcholine receptors

    … modification of the substituted cysteine with sulfhydryl reagents. These findings suggested that agonist occupation of the site at the a4(+)/a4(-) interface leads to channel gating through a coupling mechanism involving a conformational switch in loop C. The sulfhydryl reagents had similar …

    oxford-brookes Repository record for The emerging role of the a4(+)a4(-) interface as a determinant of functional signatures of a4ß2 nicotinic acetylcholine receptors (opens in a new tab)

  2. Structural and functional relationship of photosynthetic bacterial reaction centers

    … reaction centers (RCs) was modified with various sulfhydryl reagents. It was found that mercurial reagents, especially p-chloromercuribenzenesulfate (pCMBS), inhibit Q$\sb{\rm B}$ function in RCs from Rhodobacter sphaeroides and Rhodobacter capsulatus, but not in Rhodopseudomonas viridis. By …

    uiuc Repository record for Structural and functional relationship of photosynthetic bacterial reaction centers (opens in a new tab)

  3. Exploring the Dynamics of Mechanosensitive Channels Utilizing a Post Translational Modification Approach

    … allows us to use Methanethiosulfonate (MTS) sulfhydryl reagents that can form disulfide bonds with cysteines to change the properties (charge or hydrophobicity) of specific residues. We then use the double knockout strain, ∆MscL/∆MscS, which is osmotically fragile and has a reduced viability …

    utswmed Repository record for Exploring the Dynamics of Mechanosensitive Channels Utilizing a Post Translational Modification Approach (opens in a new tab)

  4. The penetration protease of the cercariae of Schistosoma mansoni

    … or phenylmethylsulfonyl fluoride, but not by the sulfhydryl reagents iodoacetamide or p-chloromercuribenzoate. Typical trypsin inhibitors such as soy bean trypsin inhibitor, trasylol or benzamidine inhibited the enzyme to a lesser degree. The active-site labels, TLCK, TPCK and AcAAAACK of trypsin, …

    cape-town Repository record for The penetration protease of the cercariae of Schistosoma mansoni (opens in a new tab)

  5. Purification and characterization of malate dehydrogenase and 6- phosphogluconate dehydrogenase from Haemophilus influenzae

    … whereas no inactivation was observed with sulfhydryl reagents. Haemophilus influenzae 6-phosphogluconate dehydrogenase was purified 308-fold with a 16% recovery through a 4-step chromatographic procedure involving a PhenylSepharose hydrophobic column, and affinity chromatography on Matrex …

    vt Repository record for Purification and characterization of malate dehydrogenase and 6- phosphogluconate dehydrogenase from Haemophilus influenzae (opens in a new tab)

  6. Animal and Plant Viruses with Double-Stranded Ribonucleic Acid

    … groupings on reovirus 3 are sensitive to sulfhydryl reagents; they are also sensitive to trypsin, but resistant to chymotrypsin; they have the ability to react with neuraminic acid-containing glycoprotein receptors, but lack neuraminidase activity. The reproductive process of reovirus 3 is …

    rockefeller Repository record for Animal and Plant Viruses with Double-Stranded Ribonucleic Acid (opens in a new tab)

  7. Mechanistic Studies on Memory of Chirality Alkylations of 1,4-Benzodiazepin-2-ones & Structure-based Design of Insecticidal AChE Inhibitors for Malaria Mosquito, Anopheles gambiae

    Memory of chirality (MOC) is an emerging strategy for asymmetric synthesis which relies upon the intermediacy of transiently non-racemic reactive species. In these reactions the configuration of the sole stereogenic center of the enantiopure starting material is "memorized" by a chiral non-racemic …

    vt Repository record for Mechanistic Studies on Memory of Chirality Alkylations of 1,4-Benzodiazepin-2-ones & Structure-based Design of Insecticidal AChE Inhibitors for Malaria Mosquito, Anopheles gambiae (opens in a new tab)