Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Sucrose gradient centrifugation"”.
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Aromatic alcohol oxidase, aldehyde oxidase and mannitol oxidase in terrestrial gastropods
… by successive rate dependent and isopycnic sucrose gradient centrifugation the mannitol oxidase fractions were analysed by electron microscopy. This revealed the predominance of tubular structures typically of seven tubules (each of approx. 50nm in diameter) in a hexagonal 6+1 arrangement …
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Comparison of Two Separation Methods for Biological Particles: Field-Flow Fractionation (FFF) and Sucrose Density Gradients
<p>Sucrose gradient centrifugation and Field-flow fractionation (FFF) are two different particle separation methods that overcome the problems of similar size microorganisms clumping together during standard filtration methods. FFF separates particles based on size and density via the parabolic …
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Surface Functionalizations towards Nucleic Acid Purification: a nanoscale study
… for polysome purification and mRNA extraction (sucrose gradient centrifugation and phenol/ethanol RNA extraction). Polysome purification on microdevice will be based on the immobilization of polysomes to the device surfaces, opportunely treated to enhance polysome adhesion. Surface …
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The growth and stress response characterization of Synechococcus WH8109 cyanobacteria
… exchange and gel filtration chromatography and sucrose gradient centrifugation. The expression levels of this chaperone were analyzed under normal and stress conditions and they have been shown to respond to heat shock and infection.
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Characterization of 50S Ribosomal Subunit Assembly Inhibition in Erythromycin Treated <em>Escherichia coli</em> Cells.
… cells.</p> <p>Subunit assembly was examined by sucrose gradient centrifugation of labeled cell lysates. Normal assembly kinetics of subunit assembly were observed in control cells at 37°C. Formation of the 30S subunit was completed by 7.5 minutes and assembly of the 50S subunit was finished by …
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The localization of UCH-L1(Ubiquitin C-terminaHydrolase-L1) in lipid rafts of neuronal cell
… was also localized in lipid rafts using the sucrose gradient centrifugation assay and soluble-in soluble fractionation assay with cold 1% Triton X-100 buffer in neuronal cell. The results showed that UCH-L1 assoicated with lipid rafts of neuronal cells. And then, we investigated whether the …
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Poly(glutamic acid) promoted assembly of nucleosome cores on the histone gene quintet of psammechinus miliaris
… by the following criteria: Their S value on sucrose gradient centrifugation, resistance to Micrococcal nuclease digestion, DNase I digestion patterns, DNase I digestion kinetics at the susceptible sites, electronmicroscopic appearance, hi stone content and electrophoretic mobility. Cores were …
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The Role of the Protein MTBP in DNA Replication
… has been carried out using gel filtration and sucrose gradient centrifugation; these identify a complex with an apparent molecular weight close to the one expected for a tetramer composed by two molecules of MTBP and two molecules of Treslin, resembling the model proposed for the interaction of …
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Structural and biochemical studies of the yeast linker histone, Hho1p
… in gel-based assays and by analytical ultracentrifugation, suggesting the GII domain may not have a major role in chromatin compaction. Chapter 4 focuses on the interaction of Hho1p with chromatin proteins. Chemical cross-linking and gel filtration indicate that Hho1p does not interact …
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The role of RNAs and extracellular vesicles in the radiation response
… using transmission electron microscopy (TEM), sucrose gradient centrifugation, sizing and the determination of EV concentration released from cells. Next-generation sequencing was performed to identify the mRNA, non-coding RNA and microRNA candidates involved. General characterisation of EVs …
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Gene Activation in Early Amphibian Development
… stage. Analysis of phenol extracted RNA by sucrose gradient centrifugation and gel filtration on Sephadex G-100 demonstrates that 70% of the RNA synthesized by early gastrulae is a high molecular weight heterogeneous RNA. Its base composition approaches that of DNA. 30% of the label appears …
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Development of novel isolation methodologies for microvesicles and exosomes, as potential biomarkers for health (Therapeutic Lifestyle Changes), and use of microvesicle-delivered β-Gly in erythroleukaemia
… by similar methodologies such as differential centrifugation of cell culture supernatant (S/N), filtration, floating on chemical gradients or using antibody labelled magnetic beads. Hence, the isolation techniques, commonly in use, provide low levels of purification and therefore require …