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Showing 1 to 19 of 19 for “"Sucrase"”.
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Effects of Glucocorticoids and Limited Nursing on the Digestive Enzyme Ontogeny and Growth of Nursing Piglets (Maltase, Sucrase, Lactase, Amylase, Acth)
Ten experiments were conducted to evaluate the inductive capacity of exogenous hydrocortisone (HYD) and adrenocorticotropic hormone (ACTH) on the developing carbohydrate digestive enzymes and growth rate of nursing piglets. The response of these enzymes to increased creep feed consumption, produced …
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INHIBITION OF ACTIVITIES OF INDIVIDUAL SUBUNITS OF INTESTINAL MALTASE-GLUCOAMYLASE AND SUCRASE-ISOMALTASE BY DIETARY PHENOLIC COMPOUNDS FOR MODULATING GLUCOSE RELEASE AND GENE RESPONSE
… [Maltase-Glucoamylase (MGAM) and Sucrase-Isomaltase (SI)], has a potentially important role in both the etiology and treatment of this metabolic disorder. Inhibition of the activities of the intestinal alpha-glucosidases may be a promising way to moderate glucose delivery to the …
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Molecular analysis of the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli
… Escherichia coli on plasmid pVS100 involves a sucrase enzyme (gene scrB), and a sucrose uptake system. Synthesis of the sucrase and sucrose uptake system in V. alginolyticus and E. coli(pVS100) is regulated. The nucleotide sequence and analysis of DNA regions encoding the sucrose uptake and …
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Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli
… and the results of a molecular analysis of the sucrase gene (scrA) are presented. The clone pVSlOO, containing a sucrose utilization system, was isolated from a genomic library of v. alginolyticus. Plasmid pVSlOO was mapped and the origin of its insert determined by Southern blotting and DNA …
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The Single drop drying of heat-sensitive materials
… from 100% to 45%. Selected enzymes, viz. dextran sucrase and invertase, were also dried and their residual activities determined by High Performance Liquid Chromatography. The residual activity of dextran sucrase was rapidly reduced at temperatures above 65°C, and the residual activity of …
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In vitro starch digestibility and ex-vivo glucose response studies of Vietnamese starchy foods using Caco-2 cells and 14C radioisotope
… inhibited α-glucosidase (32% maltase, 57% sucrase, at a concentration of 1000 µg/mL), glucose uptake (IC50 = 2300 and 2328 µM) and transport (IC50 = 2639 and 2413 µM). Anthocyanin mixture (93% Cya-3-Glu, 7% Pn-3-Glu), showed high sucrase inhibition (66% at a concentration of 1000 µg/mL), …
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Evaluation of five bifidobacterium isolates as potential probiotics and genetic analysis of their ability to withstand oxidative stress
… Bifidobacterium isolates with extremely high sucrase activity were randomly selected from a faecal sample from a healthy donor for further characterisation as potential probiotics with the ability to utilize fructo- oligosaccharide substrates in the gut. Phylogenic identification of the …
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Development and Application of a High-Throughput Assay for Discovery of Starch Hydrolase Inhibitors
… selective inhibitor of pancreatic a-amylase and sucrase. Moreover, proanthocyanidins in the extracts of root were also found to be major contributors to the inhibition of starch hydrolase. Phenylpropanoid sucrose ester compounds, smilaside A and smilaside D were isolated from extracts of the …
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Effect of Corn Quality and Enzyme Supplementation on Broiler Performance, Gastrointestinal Enzyme Activity, Nutrient Retention, Intestinal Mucin, and Jejunal Gene Expression
… were collected for determination of maltase, sucrase, and aminopeptidase N activities. Jejunal tissue on d 28 was also collected for total RNA isolation and a subsequent genome-wide microarray assay. On d 50, 54 birds per trt were processed to evaluate carcass yield. Interactions of ME and AZ …
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Early nutrition affects intestinal development and immune response in the neonatal piglet
… affect digestive enzyme activity. Lactase and sucrase enzyme activities were measured. Lactase activity was variable over time (P<0.05), and sucrase activity increased over time (p<0.05). Both of these results were not unusual and reflect normal development in the piglet. Intestinal …
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Effects of supplemental osteopontin on intestinal development and serum antibody responses to rotavirus vaccination in piglets
… regions of the small intestine. Lactase and sucrase activities of SR piglets were higher than the two formula-fed groups in the duodenum, lower in the ileum. No significant effects of diet on lactase and sucrase activities were noted between two formula-fed groups in the duodenum and ileum. …
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The role of Dietary Interventions in Broiler Intestinal Health and Immunity
… the activities of digestive enzymes maltase, sucrase and alkaline phosphatase. The expression of apoptotic cell death markers (TUNEL staining and caspase-3 expression) was not altered statistically upon incorporation of dietary additives. Receptor interacting serine/threonine kinase 3 (RIPK3) …
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Plastic Embedding Techniques for Light Microscopy Histological Studies
… separate development, the enzymes lactase and sucrase were first reported in plastic-embedded jejunum. Subsequently it was shown that these two important disaccharidases for assessing malabsorption were sensitive to specific processing agents and could easily be lost, resulting in false …
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Dietary carbohydrates influence the structure and function of the intestinal alpha-glucosidases
… the formation of a higher molecular weight (HMW) sucrase-isomaltase (SI) species in Caco-2 cells that sorts more quickly to the enterocyte surface to act as a digestive enzyme. As this finding suggested a maltose sensing ability of small intestinal enterocytes, molecular mechanisms associated with …
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Neue Ansätze in der Qualitätssicherung von Honig
Der erste Teil der Dissertation behandelt die Substanz Phenylacetaldehyd, welche im Honig ausgehend von der Aminosäure Phenylalanin als natürlicher Stoff, aber auch als Rückstand nach Einsatz als Bienenvertreibungsmittel vorliegen kann. Die in dieser Arbeit durchgeführten Untersuchungen zeigen, …
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Regulation of Intestinal Development and Function in Early-Life Pigs
… reflecting intestinal growth and remodeling. Sucrase Isomaltase (duodenum P = 0.025; jejunum and ileum P <0.001) increased from birth to weaning, coinciding with changes in disaccharidase activity. Somatostatin (duodenum, jejunum, ileum P <0.001) was greatest at birth and at weaning, …
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Developmental Regulation of the Expression of Nutrient Transporter and BrushBorder Membrane Hydrolase Genes in the Small Intestine of Piglets
… (lactase-phlorizin hydrolase, LPH, sucrase-isomaltase, SI, and maltase-glucoamylase, MGA), three peptide hydrolases (aminopeptidase A, APA, aminopeptidase N, APN, and dipeptidyl peptidase IV, DPP IV), two sugar transporters (Na+-dependent glucose transporter 1, SGLT1, and facilitated …
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The role of probiotics, prebiotics, and human milk oligosaccharides in infant formulae
… was increased 31% (p=0.030) in HT-29 cells and sucrase activity increased 54% (p=0.036) in post-confluent CaCo-2Bbe cells with 2000 mg/L 2'FL. Transepithelial resistance increased 21% (p=0.002) in post-confluent Caco-2Bbe cells with 200 mg/L LNnT. In summary, inhibition of proliferation was …
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The effects of 2’-fucosyllactose and Bifidobacterium longum subspecies infantis supplementation on growth, intestinal development and immune development and function in piglets
… piglets per kg/BW. Jejunal and ileal lactase and sucrase activities were similar between treatment groups. Histomorphology measures in jejunum, ileum and AC were similar across treatment groups, except for a trend for increased crypt width (p=0.071) and crypt volume (P=0.069) in the ileum of FL …