Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Subtractive hybridization"”.
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Uncovering novel pathogenicity-associated loci among Yersinia enterocolitica species by subtractive hybridization
In dieser Arbeit wurde die Methode der subtraktiven Hybridisierung angewandt, um neue Virulenzfaktoren zu finden, die spezifisch für hochpathogene Yersinia enterocolitica Stämme sind. Hierfür wurde die DNA eines nicht pathogenen „Treiber”-Stammes (Y. enterocolitica NF-O, Biotyp 1A) gegen die DNA …
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Draft Genome Sequencing and Suppressive Subtractive Hybridization Analysis of the Fiber -Degrading Components of Ruminococcus Flavefaciens Fd-1
*This dissertation is a compound document (contains both a paper copy and a CD as part of the dissertation). The CD requires the following system requirements: Internet Browser; Microsoft Office.
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Development of a 16S rRNA gene sequence as a biomarker specific to the gastrointestinal tract and feces of swine based on suppressive subtractive hybridization
… The amplified DNA was taken into suppressive subtractive hybridization (SSH) to enrich for swine specific sequences. The final products obtained in SSH were cloned and sequenced. Swine-specific primers were designed and tested in PCR and qPCR assays against swine and non-target DNA. Sequence …
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Differential Expression of Genes During Diapause in the Flesh Fly, <em>Sarcophaga crassipalpis</em>.
… crassipalpis</em>. The results of a Suppression Subtractive Hybridization procedure was used to indentify genes that are differentially regulated in both diapause and nondiapausing states while suppressing genes that are common to both states. Randomly picked colonies from both subtractive …
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The analysis of gene transcripts associated with conidiation in the insect pathogenic fungus, Metarhizium anisopliae /
… we used the PCR-based technique, suppression subtractive hybridization (SSH) to selectively amplify conidial-associated genes in M. anisopliae. We then identified the presence of these differentially expressed genes using the National Center for Biotechnology Information database. One of the …
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Molecular analyses of differences in gene expression due to changes in the level of dietary fat
… either a 3 or 20% corn oil (co) diet for 2 wks. Subtractive hybridization of the lowfat cDNA library revealed genes expressed preferentially by mice fed the 3% co diet. These clones are designated lowfat mammary 1 and 2 (LFM-1, LFM-2). The diet regulated LFM-1 is a homolog to an UV-inducible …
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Defining the tropism of Listeria monocytogenes using a comparative genomics approach
… namely mixed-genome microarrays, dot-blot hybridization and suppressive subtractive hybridization were used to compare the genomes of L. monocytogenes isolates of different serotypes from a variety of different sources. Over 30 genome sequences, potentially involved in the tropism of the …
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Sexual differentiation of somatic and germ cell lineages of the mouse gonad
… of gonad organogenesis, a PCR based subtractive hybridization was performed to identify mouse genes that demonstrate sexually dimorphic expression patterns during embryonic development of the testis and ovary. I successfully identified nineteen genes that are upregulated during testis …
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Investigation of the p53-dependent apoptosis versus arrest decision through analysis of Prep gene regulation
… apoptosis by the oncoprotein E1A. Through a subtractive hybridization screen, the Perp gene was identified as a transcript strongly upregulated in cells initiating p53-dependent apoptosis compared to those undergoing cell cycle arrest.
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Analysis of target genes of the transcription factor CREM during mouse spermatogenesis
… mice and wild-type mice. Using suppression subtractive hybridization we have picked 12,000 clones with sequence fragments of genes expressed stronger in wild type then in the CREM -/- mutant. 956 unique sequences were characterised. Homologies to 158 known mouse genes, 99 other known genes …
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Metabolism and gene expression during diapause in athropods
… not fully explained by oxygen limitation. Subtractive hybridization and qPCR identified 6 genes predicted to regulate diapause entry in A. socius. Reptin, TFDp2, CYP450, AKR are significantly upregulated in pre-diapause embryos, and ACLY and Capthesin B-like protease are downregulated …
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Recombinant Equine Interleukin-1 Induced Models of Equine Joint Disease
… differentiation and cell-cycle processes. Subtractive hybridization-PCR identified 148 differentially expressed cDNAs in synovium resulting from a 6-hour intra-articular EqIL-1b injection. Combined results demonstrate the potent bioactivity of our equine IL-1 proteins and support the …
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Untersuchungen zur Rolle des HIV-1-Tat-Proteins in der AIDS-assoziierten Vaskulopathie
… in Monozyten mittels der suppressed subtractive hybridization (SSH)-Methode untersucht. Hierbei wurde O-linked N-Acetylglucosamine-transferase (OGT) als Gen identifiziert, dessen Expression durch HIV-1-Tat unterdrückt wird. Bisher ist bekannt, dass OGT ein Repressor der basalen …
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Identification and Characterization of Genes Associated with <i>V-Jun</i> Induced Cell Transformation
… We have isolated several clones by subtractive hybridization, and a modified differential display procedure. One of these is clone 4, showing strong sequence homology, both at nucleotide and amino acid level, to cysteine thiol proteases. Northern blot analysis shows that the steady …
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Quitinases de tomateiro (Solanum lycopersicum L.): identificação, caracterização e atividade no controle do Fusarium oxysporum f. sp. lycopersici
… hibridização subtrativa supressiva (Suppresive Subtractive Hybridization, SSH) a partir de um genótipo resistente (genótipo BRH) desafiado com a raça 2 de Fol. Dentre os genes identificados, uma quitinase (SolChi) foi selecionada para verificar respostas no nível da expressão gênica das plantas …
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Bioinformatic and molecular approaches for the analysis of the retinal pigment epithelium (RPE) transcriptome
… in-house using a PCR-based suppression subtractive hybridization (SSH) technique (Diatchenko et al., 1996, 1999), which normalizes for sequence abundance and achieves high enrichment for differentially expressed genes. CAP3 (Huang and Madan, 1999) was used to assemble the high quality …
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Analysis of transcription factor CREM dependent gene expression during mouse spermatogenesis
… mice were compared using the suppression subtractive hybridization (SSH) technique as well as oligonucleotide DNA microarrays. SSH was used to selectively amplify the differentially expressed genes. 12,000 clones, which contain sequence fragments of genes expressed stronger in wild-type as …
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The Molecular Biology of Lichen Symbiosis and Development
… a stratified lichen thallus. Using suppression subtractive hybridization to determine differential gene expression, fungal and algal libraries were made of upregulated genes in the first 2 stages of lichen symbiosis. The symbiotic expression levels of select genes were then verified using …
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Analysis of the genome organization and fitness traits of non-pathogenic Escherichia coli strain Nissle 1917 (O6:K5:H1)
… methods such as PCR, DNA-chip technology, subtractive hybridization, transcriptomics and proteomics are of increasing importance for research on infectious diseases and public health. The aim of this work was to characterize the genome structure and -content of the probiotic Escherichia …
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Molecular dissection of Alternaria brassicicola pathogenesis mechanisms
… pathogenicity genes by creating two suppression subtractive hybridization (SSH) libraries enriched for A. brassicicola genes during fungal pathogenesis, and one full length cDNA library of genes expressed during nitrogen starvation. The first library was created to be enriched for genes expressed …
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