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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Subtilisin"”.
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Subtilisin structure and catalysis in non-aqueous media
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemistry, 1998.
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Characterization of subtilisin-like serine proteases in trichomonas vaginalis
… infection found worldwide. In this study, subtilisin-like serine proteases (subtilases) were examined for their putative role in cell viability. Published data of other eukaryotic protozoan parasites confirm the importance of subtilases for adhesion and invasion. Serine protease inhibitor …
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The New Role of Proprotein Convertase Subtilisin/Kexin Type 9: A Connection of Proprotein Convertase Subtilisin/Kexin Type 9, Apolipoprotein B, and Autophagy
… via LDL receptor (LDLR). Proprotein convertase subtilisin/kexin type 9 (<em>PCSK9</em>) is a newly discovered gene, playing an important role in LDL metabolism. Gain-of-function mutations of <em>PCSK9</em> lead to hypercholesterolemia and loss-of-function mutations of <em>PCSK9</em> are …
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PROPROTEIN CONVERTASE SUBTILISIN/KEXIN TYPE 9, ATHEROSCLEROSIS AND EXTRACELLULAR VESICLES ¿ EXPERIMENTAL AND CLINICAL EVIDENCE
… vesicles (EVs). Proprotein convertase subtilisin/kexin type 9 (PCSK9) is expressed and secreted by VSMCs. This study aimed to unravel the role of PCSK9 on VSMCs-derived EVs in terms of content and functionality. EVs were isolated from human VSMCs overexpressing human PCSK9 …
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Activity of Analogs of Anticancer Drugs on the Serine Protease Enzymes Subtilisin and Chymotrypsin
… for the anticancer activity. Chymotrypsin and subtilisin are serine proteases that have a histidine residue in the active site. We are investigating the inhibition of the digestive enzymes chymotrypsin and subtilisin by analogs of the anticancer drug cisplatin and trying to discern trends in …
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A genetic and molecular analysis of the bli-4 locus of Caenorhabditis elegans, an essential gene encoding KEX2-like subtilisin-typw serine endoproteases
This thesis reports a characterization of genetic mutations and molecular structure of the bli-4 (I) gene of Caenorhabditis elegans. The bli-4locus had been previously defined by a single recessive mutation, e937, which disrupts the structure of adult-stage cuticle causing the formation of …
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Hair Bundle Stiffness in the Turtle Utricle: Structural and Regional Variations
… to the Ca²⁺ chelator BAPTA and the protease subtilisin. BAPTA treatment reportedly breaks tip, kinocilial and ankle links while subtilisin breaks the shaft and ankle links. Following BAPTA and subtilisin treatments, bundle stiffness reduced by 65 ± 10% and 63 ± 11%, respectively. The …
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Structural and functional studies of the C-terminal domain of human apolipoprotein A-I: Limited proteolysis and deletion mutagenesis
… PC (POPC) by chymotrypsin, trypsin, elastase, or subtilisin generated a major fragment of $\sim$22 kDa. Under milder conditions proteolysis of lipid-free apoA-I produced a fragment of similar size. The fragments shared the same N-terminus as intact apoA-I and the chymotryptic fragment had a …
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Identification and characterisation of proteases in Mycobacterium tuberculosis
… typical of some serine proteases, notably subtilisins, and possibly metalloproteases. Purification attempts were unsuccessful. A family of five genes that encode putative, secreted, serine proteases has recently been described in M. tuberculosis. These proteases share 36-47% sequence …
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Defining Molecular Mechanism of Egress in Cryptosporidium
… that calcium-dependent protein kinase 5 (CDPK5), subtilisin-like serine protease (SUB1), and cGMP-dependent protein kinase G (PKG) are essential for egress of Cryptosporidium parvum merozoites from host cells. Our results demonstrated that PKG and SUB1 are crucial mediators of merozoite egress, …
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The analysis of gene transcripts associated with conidiation in the insect pathogenic fungus, Metarhizium anisopliae /
… One of the transcripts encoded an extracellular subtilisin-like protease, Prl, which plays a fundamental role in cuticular protein degradation. Analysis of the patterns of gene expression of the transcripts using RT-PCR indicated that conidial-associated cDNAs are expressed during the development …
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Microarray Analysis Identifies Novel Cholesterol Regulated Genes, Including Pcsk9 Which Regulates LDL Receptor Function and LDL Cholesterol Levels
… the down-regulated genes, Proprotein convertase subtilisin kexin 9 (Pcsk9) was cloned from mouse liver. Pcsk9 was found to be synthesized as a pro-form in the endoplasmic reticulum (ER) and expressed as a processed form in the trans-Golgi network. In mice, Pcsk9 was found to be expressed mainly …
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Characterization of PCSK9-mediated LDLR Degradation in Hepatic and Fibroblast Cells
The discovery that proprotein convertase subtilisin/kexin type 9 (PCSK9) mediates degradation of low-density lipoprotein receptors (LDLR) indicates a critical role in LDL metabolism. PCSK9 is a secreted protein that binds to the epidermal growth factor-like (EGF)-A domain of LDLR and directs the …
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Crystallographic studies on a cold adapted subtilase and proteins involved in mRNA processing
Die Kristallstruktur einer Subtilisin-ähnlichen Serin-Proteinase des psychrotrophen marinen Bakteriums, Vibrio sp. PA-44, wurde durch molekularen Ersatz bei 1,84 Å Auflösung aufgeklärt. Damit konnte zum ersten Mal die Struktur einer kälteadaptiven Subtilase aufgeklärt werden. Dies macht es möglich, …
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Mode of Action of Abscisic Acid in Barley Aleurone Layers: Induction of Proteins by Abscisic Acid
… with antibody against an $\alpha$-amylase/subtilisin inhibitor, and the 35/36 kD doublet cross-reacts with antibody against a barley lectin specific for free amino sugars. The most abundant induced protein, p29, can be greatly enriched by a combination of low pH, low concentrations of …
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Ncd Motor Tail Domain Interactions With Microtubules
… and MT co-sedimentation assays with subtilisin-digested MTs demonstrated that Ncd tail binding sites (located at the extreme C-terminus and in the H11-H12 loop / H12 helix of each tubulin monomer) on tubulin correspond to tau binding sites. Further, the Ncd tail domain, like tau, can …
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Characterization of the Non-Proteolytic Mechanism and Cellular Site of Action of PCSK9-Mediated Degradation of the Low-Density Lipoprotein Receptor
Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) is a serine protease that has emerged as a central regulator of plasma low-density lipoprotein cholesterol levels. Here, it is demonstrated that PCSK9 is secreted into the blood and that the secreted PCSK9 binds and degrades LDLRs in liver. To …
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Structure Determination of Viscotoxin A1, Tendamistat and Tri Peptidyl peptidase-I
… 2.35Å ergab eine globuläre Struktur mit einer Subtilisin-ähnlichen Faltung, einer S475-E272-D360 katalytischen Triade und einer oktaedrisch koordinierten Ca2+ Bindestelle, die charakteristische Eigenschaften der S53 Familie der sedolisinartigen Peptidasen sind. Im Gegensatz zu anderen S53 …
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Somatic cell gene therapy for diabetes mellitus: engineering a surrogate B-cell
… to the consensus sequence for cleavage by the subtilisin-like serine protease, furin, was carried out. Co-transfection of human fibroblasts with wild-type proinsulin and furin resulted in 58% conversion to mature insulin by these cells. Intraperitoneal implantation of the mature-insulin …
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