Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 37 for “"Substrate selectivity"”.
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Autoregulatory and structural control of CaMKII substrate specificity
… and synaptic plasticity. CaMKII exhibits broad substrate specificity, targeting numerous substrates with diverse phosphorylation motifs. Binding of the calcium sensor CaM to the autoregulatory domain (ARD) of CaMKII functions to couple kinase activation with calcium signaling. Important sites of …
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Molecular Characterization and Loss-of-Function Analysis of an Arabidopsis thaliana Gene Encoding a Phospholipid-Specific Inositol Polyphosphate 5-Phosphatase
… of inositol phosphate and phosphoinositide substrates. I have examined the substrate specificity of the At5PTase11 protein from the model plant, Arabidopsis thaliana. The At5PTase11 gene (At1g47510) encodes an active 5PTase enzyme that can dephosphorylate the phosphoinositide substrates …
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Insights into the role and mechanism of the AAA+ adaptor Clps
… in their function, especially with respect to substrate selectivity. Adaptor proteins that can associate with the proteases add an additional layer of control to substrate selection. Thus, understanding the mechanism and role of adaptor proteins is a critical component to understanding how …
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Molecular Mechanisms of Gating, Selectivity, and Transport in Membrane Channels and Carriers
… cells. Using molecular dynamics simulations, the substrate selectivity, gas permeability and gating mechanism of AQPs, as well as the substrate binding and induced translocation in AAC, are investigated. Two AQPs from the same organism with different functionalities are used to identify key …
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Characterization of CAXCK31, a Bacterial Calcium/Proton Antiporter
… transport properties of CAXCK31, including substrate selectivity, pH dependence, and transport rates, have been characterized for the first time.</p>
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Investigation of regulatory and functional diversity in an enzyme superfamily
… members of this subgroup exhibit distinct substrate specificities, some members with low sequence identities display identical activities. Though the underlying causes of these phenomena are still unknown, evolution of either regulatory and/or functional mechanisms could have generated …
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Biochemical Studies of Aromatic Amino Acid Decarboxylases and Acetaldehyde Synthases
… enzymes with the first reaction step at substrate Cα position, including decarboxylase and acetaldehyde synthase. L-3, 4-dihydroxyphenylalanine (L-dopa) decarboxylase (DDC) is a model enzyme we use as a reference because the structures and functions of DDC are relatively well established. …
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Nucleic base-directed adsorption of colloids and polyelectrolytes
… adsorption in which polyelectrolytes of known substrate selectivity control the adsorption of the colloids onto which they are layered. The first section of this thesis will describe the evolution of this technique in response to certain limitations inherent to the patterning of multilayered …
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The synthesis and evaluation of valine mimic protio and halo enol lactones as human neutrophil elastase inhibitors
… lung tissue in emphysema. Based on the primary substrate selectivity of HNE, a series of valine mimic protio and bromo enol lactones were designed as HNE inhibitors. General methods were developed for the preparation of $\alpha$- and $\beta$-alkyl-substituted 5-alkynoic acids by the bromoform …
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Interplay of DGAT1, PDAT1 and DGAT2 Enzymes in Plant Triacylglycerol Assembly
… that each enzyme prefers DAG and acyl-CoA substrates with different fatty acid compositions. Industrially desirable seed oil composition can be obtained through genetic engineering by replacing the endogenous enzyme with one that has different substrate selectivity. In <em>Arabidopsis …
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Synthetic and metabolic studies on centrally acting amines
… N¹⁰-formyltetrahydrofolic acid. (3) Extensive substrate-activity studies have established that only those 1,2,3,6-tetrahydropyridine derivatives bearing a lipophilic C-4 substituent and an N-methyl group are likely to be good monoamine oxidase B substrates. The design and synthesis of potential …
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Study of Copper Nanowires With Five-Twinned Structure Grown by Chemical Vapor Deposition and Their Applications
… also be selectively grown on a patterned Au/Si substrate. Single CuNW was selectively grown on the nanoscale-patterned substrate. Selectivity was achieved with the aid of phosphite passivation of the gold surface. The temperature dependent growth of CuNWs confirmed the role of phosphite on …
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Development And Optimization Of The First High Throughput In Vitro Fret Assay To Characterize The Saccharomyces Cerevisiae Gpi-T
… glutathione-S-transferase (GST) domain. Peptide substrates for GPI-T were synthesized and modified to contain a pair of chromophores suitable for the development of a fluorescence resonance energy transfer (FRET) assay. GPI-T activity was observed as a time-dependent increase in fluorescence by …
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Decoding the structure WWP2 in the TGFβ signalling pathway
… of WWP2, and to explore how this relates to the selectivity of different isoforms in the TGFβ pathway. Overexpression of recombinant WWP2 domains in a bacterial host, and affinity and sizeexclusion chromatography have been used to produce pure, high concentration protein samples. Both NMR …
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Using VHHs to study the function and control of the E2 Ubiquitin-conjugating enzyme UBC6e
… clearance not only of terminally misfolded substrates, but also of wild-type glycoproteins that fold comparatively slowly. Tuning of ERAD component level involves a mechanism that is likely distinct from the conventional ERAD by Hrdl/SEL1L complex. To better understand how UBC6e is …
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Relationship Between Flavonoid Structure And Phase-II Metabolism
… the major organ of metabolism; 2) establish the substrate-selectivity and regiospecificity of these major UGT isoform(s); 3) develop the in silico prediction models for UGT 1A8 and UGT1A9 using pharmacophore and 2-D/3-D QSAR modeling techniques; 4) study the effect of change in backbone on the …
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A Selectivity Study on the Use of Caffeine and Theobromine Imprinted Polypyrrole Surface Electrodes
… prepare recognition matrices that possess high substrate selectivity and specificity. Conducting polymers (CP) have proved to be an excellent tool for the preparation of nano-structured biologically selective systems. Polypyrrole (Ppy) is one such CP that is extensively used for the construction …
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Investigating the mechanisms and outcomes of Sprouty-2 S-acylation
… enzymes that display distinct activity and substrate selectivity profiles. Sprouty (SPRY) proteins are regulators of Receptor Tyrosine Kinase (RTK) signalling and play a major role in maintaining cellular homeostasis. Indeed, their deregulation is linked to tumorigenesis, developmental …
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Ribonucleotide reduction and redox regulation in Archaea : surprising twists on a common theme
… is the first instance of a RNR displaying dual substrate selectivity. Our investigation of redox regulation focused on thioredoxin reductase (TrR) and thioredoxin (Tr). In addition to providing the reducing equivalents for many RNRs, TrRs are involved in maintaining the intracellular redox …
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The mechanism of Parkin activation by PINK1 phosphorylation
… Ub from upstream E2 Ub-conjugating enzymes to substrates on the mitochondrial surface via an E3$\sim$Ub intermediate. $\\$ Resolving the precise mechanism of Parkin activation by PINK1-mediated phosphorylation is the primary aim of this thesis. Additionally, building on previous work in which a …
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