Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"Substrate Stiffness"”.
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Effects of substrate stiffness on bacterial biofilm formation
… is known to be influenced by many properties of substrate materials, such as surface chemistry, hydrophobicity, roughness, topography, and charge. However, few studies have been conducted to investigate the effects of substrate stiffness. In this study, Escherichia coli RP437 and Pseudomonas …
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Role Of Substrate Stiffness In Driving Atherogenic Behavior In Macrophages.
… properties of tissue, the effect of physical stiffness and associated matrix remodeling on macrophages can now be studied. Herein I describe the effects of physical substrate stiffness on macrophage behavior relevant to atherosclerosis plaque formation using a polyacrylamide hydrogel-based in …
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Substrate stiffness tunes the neuronal response to the chemical guidance cue semaphorin3A
… the Franze research group identified that tissue stiffness and the mechanosensitive ion channel Piezo1 are important for proper pathfinding of the axons of retinal ganglion cell neurons (RGCs). In an in vitro culture system comprising explanted Xenopus laevis eye primordia, I found that …
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Role of Substrate Stiffness on Migratory Properties and Epithelial to Mesenchymal Transition in Human Lung Cancer Cells
… process in cancer cell metastasis, the role of substrate stiffness on EMT and motility patterns is not completely understood. In addition, EMT can be induced in some cell types transforming growth factor-¿¿(TGF-¿¿)but it is not known how changes in substrate stiffness influence TGF-¿¿ induced …
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Stiffness controls primary cilia position and length: mechanisms and potential in reversing osteoarthritis phenotypes
The focus of this thesis was to determine how the stiffness of the extracellular microenvironment influences cellular mechanosensitivity through the action of, the primary cilium. Mechanical stress and the stiffness of the extracellular matrix are key drivers of tissue development and homeostasis. …
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Genetic Regulation of Retinal Cells by Variation of Underlying Elastic Modulus
… it was noticed that when cells are plated on substrates of different stiffnesses undergo certain conformational changes in their cytoskeleton. Specifically, the tension in cytoskeleton of the cells on the substrates increased with stiffness. Many different cell types were noted to have a …
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Cells and force transduction
… tension gauges to investigate how force and substrate stiffness guide cellular decision-making during initial cell attachment and spreading on deformable substrates. The well-established dependence of cell traction and spreading on substrate stiffness has been attributed to levels of force …
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The mechanical regulation of Semaphorin3A signalling in the developing Xenopus laevis brain
… guidance cue Semaphorin3A is regulated by substrate stiffness. The strength of axonal turning responses to Semaphorin3A can be quantified using a turning assay, whereby axons change their trajectory in response to a gradient of a chemical cue. I developed two alternative turning assay …
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Probing cellular mechano-sensitivity using biomembrane-mimicking cell substrates of adjustable stiffness
… recognized that mechanical properties of substrates play a pivotal role in the regulation of cellular fate and function. However, the underlying mechanisms of cellular mechanosensing still remain a topic of open debate. Traditionally, advancements in this field have been made using …
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Engineering Matrix Properties for Stem Cell Culture and Gene Delivery
… a synthetic stem cell niche with controlled stiffness and density of cell adhesion peptides was developed for spermatogonial stem cell (SSC) culture (Chapter 2). The synthetic stem cell niche allowed for the growth of SSCs in both two-dimensional (2D) and three-dimensional (3D) cultures, and …
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The mechanical regulation of Eph/ephrin signalling in the developing Xenopus brain
… understood, I investigated the role of tissue stiffness in this signalling pathway using the optic pathway of the African clawed frog, Xenopus laevis, as a model system. Using atomic force microscopy, I found that, in vivo at early stages, the Xenopus optic tectum was soft and mechanically …
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Molecular Mechanisms of Lymphocyte Activation - Please Mind the Gap
… activation mechanism in lymphocytes. Finally, stiffness-dependent T cell activation is explored. While substrate stiffness is known to influence T cell activation,studies are typically performed on glass, which is orders of magnitude stiffer than any substrate in vivo. This work introduces a …
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Applications of Traction Force Microscopy in Measuring Adhesion Molecule Dependent Cell Contractility
… hydrogels as controlled elastic modulus substrates for single cell traction force microscopy studies. The first section describes the use of EDC/NHS chemistry to convalently link microbeads to the hydrogel matrix for the purpose of performing long-term traction force studies (7 days). The …
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Experimental and Computational Analysis of Cell Mechanics during Spreading and Migration
… using patterned micropost arrays, the effects of substrate stiffness, cell adhesive area, and micropost density on contractile forces of endothelial cells were investigated. Each of these traits was found to play a fundamentally different role in contributing to overall cellular contractility. …
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Enhancing the Performance of Skeletal Muscle Powered Biohybrid Robots
… cells delaminating from their underlying substrate. Building on previous work from our lab demonstrating a method to culture contractile skeletal muscle in 2D formats, this work aims to enhance the performance of these systems by tuning substrate stiffness and topography. We show that …
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Biophysical and gene expression change in living cells by force-induced mechanotransduction
… F-actin, cytoskeletal tension, Lamin A/C, or substrate rigidity. Other protein pairs in Cajal bodies exhibit different magnitudes of fluorescence resonance energy transfer. Dynamic cyclic force induces tiny phase lags between various protein pairs in Cajal bodies, suggesting viscoelastic …
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Influence of static and dynamic topography on osteoblast proliferation and maturation
… topography was created on polymers of varied stiffness to determine if there is correlation between substrate stiffness and topography on osteoblast maturation. Overall, this thesis gives insight into potential benefits of SMP use in biomedical applications. In addition, it demonstrates the …
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Buckling patterns on pre-stretched bilayer shells
… our samples, releasing the pre-stretch in the substrate by deflation places the outer film in a state of biaxial compression and yields a variety of buckling patterns. We systematically explore the parameter space by varying the pre-stretch of the substrate and the ratio of film stiffness to …
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Engineering approaches to study lung mechanobiology
… of cyclic stretch. I further varied the cell substrate stiffness in order to replicate the mechanics of soft and healthy tissue, or stiff, fibrotic tissue. This device also enables dynamic imaging of cells subject to mechanical stretch. Studies conducted with this device (Chapter 2) …
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Kinetics of cell attachment and spreading on hard and soft substrates
… from suspension first comes into contact with a substrate, it forms initial attachment bonds with proteins on the substrate surface. These bonds are mediated through integrins, which are transmembrane heterodimers, binding to the cell's environment on one side and to the cell's cytoskeleton on …
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