Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 8 of 8 for “"Substituted cysteine accessibility method"”.
-
Investigation of Prestin Membrane Topology by Substituted Cysteine Accessibility Method.
… of the two published topology models. A methodology that has not yet been used to study prestin’s topological map is substituted cysteine accessibility method. This method requires the introduction of cysteine residues into predicted extracellular or intracellular loops of prestin. …
-
Investigation into the role of binding loop E on the function of the nematode cys-loop GABA receptor.
… acid residue in binding loop E was changed to a cysteine and analyzed via electrophysiology and the substituted cysteine accessibility method. It was found that of the 18 loop E mutants analyzed, His142, Ser144, Arg147, and Ser157, all played a role in channel activation and were sensitive to …
-
Regulation of Expression and Function of Neuronal Nicotinic Receptors by Accessory Subunits
… separable ligand binding properties using a substituted-cysteine accessibility method followed by covalent modification. This approach identified a third ligand binding site unique to the (α3)3(β4)2 stoichiometry at the α3 accessory interface that is crucial for channel activation. …
-
<em>Agrobacterium</em> Virb10 Contributions to Type Iv Substrate Secretion, T-Pilus Biogenesis, and Outer Membrane Pore Formation
… large C-terminal modified β-barrel. I introduced cysteine insertion and substitution mutations throughout the length of VirB10 in order to: (i) test a predicted transmembrane topology, (ii) identify residues/domains contributing to VirB10 stability, oligomerization, and function, and (iii) monitor …
-
Characterization of The Agrobacterium Tumefaciens Virb2 Pilin of The Virb/D4 Type Iv Secretion System
… linked N and C termini. VirB2 was shown by the substituted cysteine accessibility method (SCAM) to adopt distinct structural states when integrated into the inner membrane and when assembled as a component of the secretion channel and the T-pilus. The VirB4 and VirB11 ATPases were shown by SCAM …
-
Determining Structural Transitions That Occur Upon Gating a Bacterial Mechanosensitive Channel
… to solve this problem. The first utilized the Substituted-Cysteine Accessibility Method (SCAM). Specific amino acids are replaced with a cysteine residue and the ability of that cysteine to react with a substrate is assessed. A cysteine library of the transmembrane domains of E. coli MscL was …
-
Identificaiton of the pore-lining helices of connexin 43 gap-junctional hemichannels
… poly-alanine helix scanning mutagenesis, the substituted cysteine accessibility method and luminescence resonance energy transfer. Using the latter methodology in particular, as well as a new method to produce purified hemichannels of controlled subunit composition, I was able to assign all …
-
Molecular transport properties of sodium/dicarboxylate cotransporters
… half of TM3 in NaDC1 was studied using the substituted-cysteine accessibility method and the transport specificity ratio (TSR). The TSR analysis provides evidence that TM3 contains determinants for substrate specificity and catalytic efficiency. All of the mutants were tested for sensitivity …