Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"Subcellular Fractionation"”.
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Subcellular Fractionation Methods for the Quantification of Endogenous Protein in Human Liver Using Targeted Proteomics
… enrichment strategy is often employed, i.e., subcellular fractionation. This method allows the enrichment of targeted proteins based on their subcellular location by isolating the corresponding organelles. In this dissertation, subcellular fractionation methods were incorporated into the …
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Glycosyl Transferases in Chondroitin Sulfate Biosynthesis: Subcellular Location and Elongation Reaction
The subcellular location and polymerization reaction of the glucuronosyl transferase and the N-acetylgalactosaminyl transferase involved in the synthesis of chondroitin sulfate were examined in cultured embryonic chick tibial chondrocytes. The chondrocytes were subjected to subcellular …
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The regulation and role of oxidative signal-inducible 1 protein kinase in Arabidopsis thaliana
… OXI1 protein kinase. Confocal microscopy and subcellular fractionation studies revealed a cytosolic localisation pattern for OXI1. Employment of a bioinformatics approach confirmed the induction of OXI1 gene expression in response to a range of AOS generating stimuli. However, the …
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Fractionation and compositional studies of rabbit skeletal muscle membranes
… constituents, which include membrane-enclosed subcellular structures known as organelles. The cells of most tissues also contain a reticulum or system of branching tubules. The interplay of the biochemical activities of these structures enables the cell to function. Almost thirty years ago, …
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Kinetics and Intracellular Pathways Required for MHC Ii-Peptide Loading and Surface Expression of a Hapten-Protein Conjugate in Murine Macrophage
… several techniques including flow cytometry and subcellular fractionation, new insights into the cell biology of antigen processing in murine macrophage were revealed. Specifically, the proteolytic enzymes involved in the cleavage of the antigen act at different points within the endocytic system …
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Brain distribution and release of Cholecystokinin octapeptide
… throughout brain, particularly in the cortex. Subcellular fractionation of rat brain was used to study the subcellular localisation of CCK. Tissue was homogenised to shear off nerve terminals (synaptosomes) which were purified and used to study the release of the peptide from hypothalamic and …
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Nrf2-Dependent Overexpression of Sulfiredoxin and Peroxiredoxin III in Human Lung Cancer, Especially Squamous Cell Carcinoma
… 8 G. Two-dimensional electrophoresis 9 H. Subcellular fractionation 10 I. Immunohistochemistry 10 J. Statistical analysis 11 Ⅲ. RESULTS 12 A. Localization of Prx III and Srx in A549 cells 12 B. Oxidation of Prx III in mink lung epithelial cells 12 C. Srx dependent regulation of Prx III …
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Aromatic alcohol oxidase, aldehyde oxidase and mannitol oxidase in terrestrial gastropods
… (n-hexadecyl) were utilised by this activity. Subcellular fractionation experiments showed aromatic alcohol oxidase activity to be localised in the endoplasmic reticulum. Aldehyde oxidase activity was considered in relation to its possible role in the further utilisation of the products of …
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The Molecular Anatomy of Mitochondrial Disease: Identification and Characterisation of Novel Nuclear-Encoded Mitochondrial Disease Genes
… A combination of super-resolution microscopy and subcellular fractionation and protease digestion studies confirmed the intracellular localisation of COA7 to be the mitochondrial intermembrane space. Secondly, a novel homozygous recessive variant in TMCO6 (NM_018502.5: c.271C>T) was identified in …
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GPR50, a potential factor involved in psychiatric disorders interacts with Alzheimer’s disease-related protein β- secretase (BACE1)
… at the neurites in mouse primary neuronal cells. Subcellular fractionation of adult mouse brain revealed that endogenous Gpr50 and Bace1 were co-fractionated in the presynaptic vesicles. Secondly, I showed that, in contrast to HEK293 cells, GPR50 overexpression had no effects on β-secretase …
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Identification and characterization of innate immune receptor substrates of γ-secretase enzyme complex
… we investigated the spatial distribution, subcellular trafficking and subcellular occurrence of regulated intramembrane proteolysis of IL-1R1, TLR4 and TNFR1. Using dynasore an inhibitor of clathrin-dependent receptor endocytosis, both ectodomain shedding and γ-secretase-mediated cleavage …
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Interrogations of protein and RNA: localisation, interactions and dynamics
… transcriptome. In this Thesis, two biochemical fractionation-based techniques were developed in the quest for subcellular exploration of the proteome and transcriptome. In addition, two biological questions were investigated with regard to the post-transcriptional control of the ultraviolet …
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Epigenetic Modulation of Synaptic Resilience in Alzheimer’s Disease
… signatures characteristic for these groups. Subcellular fractionation followed by 2D gel electrophoresis and mass spectrometry were used to analyze the PSDs. Fifteen proteins were identified as the unique proteomic signature of NDAN PSDs, thus setting them apart from control subjects and AD …
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Characterization of the Saccharomyces cerevisiae gene MGP1, a novel member of the ras gene superfamily that functions in the mitochondrial biogenesis
… MGP1;Mgp1p co-fractionated with mitochondria in subcellular fractionation experiments. In vitro mitochondrial import experiments demonstrated Mgp1p is translocated into mitochondria by a mechanism dependent on inner membrane potential, and Mgp1p was processed during translocation. After …
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A signal for retention of cytochrome P450 2C1 in the endoplasmic reticulum
… and cellular localization was determined by subcellular fractionation, immunolocalization and the glycosylation state of the proteins. Both the $\beta$-galactosidase and alkaline phosphatase hybrid proteins were retained in the endoplasmic reticulum establishing that a specific sequence or …
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Tyrosine Sulfation in A431 Cells
… membrane proteins. Further, I developed a subcellular fractionation method for A431 cells and employed enzyme marker assay and electron microscopy examination to characterize the subcellular fractions. The sulfated P61 and its sulfotransferase were localized to the rough endoplasmic …
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Role Of Type Ii Phosphatidylinositol 4-Kinases In Endosomal Tubule Dynamics During Melanosome Biogenesis
… not affect BLOC-1 association with membranes by subcellular fractionation. Rescue studies revealed that enzymatic activity and AP-binding abilities of both PI4KIIα and PI4KIIβ are necessary for TYRP1 localization to melanosomes. Live cell imaging showed that PI4KIIα associates with tubules from …
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NSAIDs Modulate Morphine Transport at the Blood-Brain Barrier: A Role for P-glycoprotein
… a drug-drug interaction. Confocal microscopy and subcellular fractionation revealed that under conditions of PIP, the disassembly of high-molecular weight P-gp-containing structures result in an increase in P-gp ATPase activity and changes in the localization of P-gp. Western blot analysis …
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Novel roles of striatal enriched protein phosphatase (STEP) in neuronal intrinsic properties and homeostatic synaptic plasticity
… neurons. In the part of appendix, I describe subcellular fractionation technique using hippocampi from rats which underwent ECS, and molecular and electrophysiological verification of chemical long-term potentiation (cLTP). Taken together, the findings in this dissertation suggest that STEP …
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The role of Nbeal2 in the homeostasis and retention of granules in haematopoietic cells
… related processes in haematopoietic cells. Subcellular fractionation of human and murine platelets revealed that Dock7, a known Nbeal2 binding partner, is mislocalised and has reduced expression in the platelets of GPS patients and Nbeal2−/− mice. The interaction of Nbeal2 with Dock7, a …
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