Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Stx2"”.
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Factors regulating the production of STX-2 in Escherichia coli O157:H7
… to determine their effect on expression of the stx2 gene and on release of Stx-2 from the pathogen. Those factors selected for study were: (i) various classes of antibiotics; (ii) probiotic microorganisms; and (iii) carbon source variation together with cAMP. Stx-2 was assessed using MTT …
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Zelluläre Funktionsstudien zum Wirkungsmechanismus von Shiga Toxinen bei mikro- und makrovaskulären Endothelzelllinien
… der Stx umfasst zwei Hauptgruppen, Stx1 und Stx2, welche 53 bzw. 64 Prozent Sequenzidentität in ihrer A- bzw. B-Untereinheit aufweisen. Nach der Freisetzung der Stx durch die EHEC im Lumen des Gastrointestinaltraktes gelangen diese über den Blutkreislauf zu ihrem Wirkungsort, den kapillaren …
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Determining the Roles of Q-SNAREs in Mast Cell Tumor Necrosis Factor Exocytosis
… to SNAREs in TNF release in mast cells, I used Stx2 KO BMMCs as well as Stx3 and Stx11 KD RBL-2H3 cells in secretion assays to determine the consequences of depleting these isoforms on TNF secretion. The resulting data indicates that while Stx2 KO does not impact either TNF secretory pathway, …
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Mechanismen der Apoptose in proximalen Tubulusepithelzellen der Niere (LLC-PK1) : ein pathophysiologisches Modell für das hämolytisch-urämische Syndrom (HUS)
… Shigatoxin1 (Stx1) und als auch Shigatoxin2 (Stx2) toxisch auf proximale Tubulusepithelzellen der Niere (LLC-PK1) wirken und konzentrationsabhängig Apoptose auslösen: [Kontrolle: 10%, Stx1 0,1µg/ml: 14% (p>0,05), Stx1 0,5µg/ml: 29% (p<0,001) , Stx1 1,0µg/ml: 37% (p<0,001), Stx2 0,1µg/ ml: 21% …
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Untersuchungen zur Regulation von Shiga-Toxin 2 und zur Attenuierung von enterohämorrhagischen Escherichia coli
… Strategien mit dem Ziel verfolgt, einen Stx2-produzierenden EHEC-Stamm zu attenuieren. Eine Attenuierungsstrategie für EHEC ist die direkte Ausschaltung von Virulenzfaktor-Strukturgenen wie den Toxingenen. Zu diesem Zweck wurde eine stx2-negative Mutante des EHEC-Stammes O157:H7 86-24 …
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Jautienoje aptiktų Šiga toksiną išskiriančių Escherichia coli identifikavimas ir genominė analizė /
… characterized by the synthesis of Stx1 and/or Stx2 toxins. STEC is associated with numerous human diseases worldwide, ranging from mild diarrhea to hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). Cattle are considered the primary source …
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Isolation And Molecular Characterization Of Shiga Toxin Producing Escherichia coli In Cattle, Water And Diarrhoeal Children From The Pastoral Systems Of Southwestern Uganda
… of STEC from di erent sources produced Stx1 or Stx2 or both Stx. Using PCR or PCR-RFLP assays, stx2 and eae gene types were analysed. Variant stx2 vhc was most prevalent and closely associated with stx2d2 in clinical and bovine STEC. The frequency of eae-positive STEC among clinical and bovine …
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Development of 11-plex assay for the rapid screening of samples for detection of Shiga toxin-producing E. coli
… O157:H7 as well as the virulence genes: stx1, stx2, and eae. The goal is to produce a multiplex panel of MOL-PCR probes for identifying DNA signatures corresponding to each of the STEC-8 serotypes and ultimately strain specific identification.'
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Recombinant Mucins with Tailored Glycosylation as Bacterial Toxin Inhibitors
… receptors of Shiga toxin 1 and 2 (Stx1 and Stx2) and C. difficile toxin A, respectively. The blood group P1 determinant generated in multiple copies on PSGL-1/mIgG2b by the expression of pigeon α4GalT and the core 2 enzyme (C2β6GnT1) bound with high avidity to both Stx1 and Stx2. In Paper …
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Shiga Toxin-Producing Escherichia coli: a Public Health Challenge in the Pre-Harvest Stage of the Farm-to-Table Continuum
… of strains positive for virulence genes stx1, stx2, eaeA, and hlyA; (c) isolate and identify serotypes present in stx-positive samples; and (d) assess genetic similarity of isolates. Understanding factors that influence distribution of STEC strains may help support on-farm management strategies …
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WATER BUFFALO MILK CHAIN IN BANGLADESH
… for E. coli O157:H7 were tested, eae, stx1, and stx2, of which the stx2 genotype was the most prevalent in milk products (74 %). L. monocytogenes and Y. enterocolitica were more prevalent in the farm bulk milk (65-79 %) than in the later milk value chain nodes. The prevalence of S. enterica was …
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Effects of low molecular weight compounds on growth, virulence gene expression and cytotoxic activity of Shiga toxin-producing Escherichia coli O157:H7
… expression of the Shiga toxin genes, stx1 and stx2, and the intimin gene, eaeA, by STEC serotypes (O157:H7 and non-O157), and to investigate the effect of compound NV002 on human kidney (HK-2) cells based on the influence of the compound on the expression of major virulence genes in E. coli …
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Characterization of Campylobacter, Salmonella, and Diarrheagenic Escherichia Coli from Food, Food Waste and Water in the Chobe Region of Botswana
… and enteropathogenic E.coli), stx1 and stx2 (present in enterohemorrhagic E. coli) and est1b ( present in Enterotoxigenic E.coli) genes. Campylobacter isolates were confirmed using a genera-specific PCR while S. enterica isolates were confirmed using invA primers. These enrichment and …