Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 564 for “"Structural biology"”.
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Structural biology of GroEL assisted protein folding
… related to GroEL-assisted protein folding using structural biology methods. First, how does GroEL capture SP and how does SP change the kinetics of ADP release? Second, how does GroEL/ES encapsulate SP and control the duration of SP encapsulation? Chapter 1 summarizes the ATPase cycle of GroEL …
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Structural Biology in Cellular Environments Using Sensitivity Enhanced NMR
This dissertation describes the general applicability of DNP solid state NMR to mammalian cells. DNP NMR can detect proteins at their physiological concentrations within cellular environment in tractable times. However, these experiments were limited to cellular lysates, which did not recapitulate …
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Mycobacterium tuberculosis strains in New Zealand: phylogeny and structural biology
… Here, whole genome sequencing, phylogenetics and structural biology were used to investigate evolutionary origins and functional consequences of genomic diversity in New Zealand M. tuberculosis clusters, with a particular focus on the Rangipo strain. Analysis of Rangipo strain non-synonymous …
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Drug Discovery in Redox Biology: From Natural Products to Structural Biology
… infectious diseases. Natural products provide structurally diverse redox-active scaffolds capable of modulating oxidative stress and redox sensitive signaling pathways. The genus Rinorea (Violaceae) is metabolically diverse yet remains largely underexplored. Rinorea longiracemosa (Kurz) Craib, …
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Structural Biology of Cytochrome P450 Monooxygenases in Arabidopsis Thaliana Phenylpropanoid Pathway
… modes. These experiments have clarified the structural specificity of CYP98A3 for p-coumaroyl shikimate and indicated a possible substrate access channel. Procedures have also been developed for the expression of N-terminally truncated forms of CYP98A3 and CYP73A5 suitable for x-ray …
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Modeling proteins, making scientists : an ethnography of pedagogy and visual cultures in contemporary structural biology
… and pedagogy in the burgeoning field of structural biology. Structural biologists are a multidisciplinary group of researchers who produce models and animations of protein molecules using three-dimensional interactive computer graphics. As they ramp up the pace of structure determination, …
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New Tools for Structural Biology and Biophysics: High-Throughput Fluorine Solid-State NMR and Applications to Membrane Proteins
Solid-State Nuclear Magnetic Resonance (SSNMR) spectroscopy is a powerful method for characterizing the structure and dynamics of crystalline and amorphous solid compounds, materials, and biological systems. When applied to biomolecular systems such as membrane proteins, it can provide access to …
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Exploring the molecular architecture of proteins: Method developments in structure prediction and design
… of genotype, proteins have been a focus in structural biology. Since the first characterization and structure determination of protein molecule more than half a century ago1, our understanding of protein structure is improving only incrementally. While computational analysis and experimental …
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ADVANCING IN SITU CRYO-ET TO THE NEXT DIMENSION: TOMOGRAPHY ON 3D ORGANOIDS
… tissue function remains a central challenge in biology. In situ cryo-electron tomography (cryo-ET) can help to address this challenge by providing high-resolution, three-dimensional visualization of macromolecules in near-native, minimally disturbed conditions. With its broad resolution, cryo-ET …
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Machine learning for cryo-EM structure determination
… cryo-microscopy (cryo-EM) has revolutionised structural biology thanks to its relaxed sample requirements. Recent advances in cryo-EM image processing have enabled the structural determination of many macromolecules that were previously intractable by other methods. At the same time, …
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APPLICATION OF STRUCTURAL, MOLECULAR, AND PROTOEMIC STUDIES TO INVESTIGATE ON-TARGET AND OFF-TARGET MECHANISMS OF LORLATINIB RESISTANCE IN ALK DRIVEN NEUROBLASTOMA
… of this thesis, I describe how we applied structural biology along with cell based assays and patient derived xenograft models (PDX) to uncover mechanism of on-target resistance caused by acquired secondary mutations in ALK kinase domain. In the third chapter, I describe how we applied …
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Methods for increasing model accuracy and simulation time scales of biological processes with molecular dynamics
… and the central role they play in the field of structural biology is introduced to give the reader some background on the common basis of the projects. The second chapter describes the first of these projects, where the molecular dynamics flexible fitting method for refining molecular structures …
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Computational approaches for the design and prediction of protein-protein interactions
… a large class of applications in computational structural biology for which atomic-level representation is crucial for understanding the underlying biological phenomena, yet explicit atomic-level modeling is computationally prohibitive. Computational protein design, homology modeling, protein …
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Protein Shape Description and its Application to Shape Comparison
… general. This fact has been the basis for many structural biology methods including the molecular replacement method used in X-ray crystallography or homologous structure restraints in the refinement methods. With the success of methods based on prior information, it is feasible that novel …
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Computational dissection of nitrate transport mechanism
… transporters. Despite recent breakthroughs in structural biology, our knowledge of the molecular nature and regulation of nitrate transporters is far from complete. The major reason is that structural biology only provides few ""snapshots"" of protein structures, whereas proteins undergo …
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Development of Quantitative Solid-State NMR Methods to Characterize Membrane Proteins
… which make many techniques commonly used in structural biology extremely challenging. Membrane proteins often display a structural dependence on the local environment. It is therefore essential to have structural biology tools to study these critical proteins in native-like environments. …
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A thermodynamic definition of protein folds
Modern techniques in structural biology, like homology modeling, protein threading, protein fold classification, and homology detection have proven extremely useful. For example, they have provided us with evolutionary information about protein homology which has in some many cases lead directly to …
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DEVELOPMENT OF VIRTUAL SCREENING METHODOLOGIES FOR IDENTIFYING RNA-BASED THERAPIES
… and enhancing the field of computational structural biology.
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Insights into structure, dynamics of thioredoxin reassembly and dynein light chain 8 and ionization states of thiamin cofactor by MAS NMR spectroscopy
… developing as a method for atomic-resolution structural characterization of proteins and protein assemblies that are insoluble and difficult to crystallize. A number of laboratories including ours are working on developments of MAS technologies that would enable analysis of complex biological …
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