Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Stress Granule"”.
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Ruolo della TDP-43 nella formazione dei granuli da stress nella Sclerosi Laterale Amiotrofica
… Recent studies suggest that TDP-43 may regulates stress granules dynamics that are cytoplasmic structures composed of non-translating messenger ribonucleoproteins (mRNPs) that rapidly aggregate in cells exposed to adverse environmental conditions. Stress granules function in part to triage RNA and …
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STRESS GRANULES MODULATE SYK TO CAUSE MICROGLIAL DYSFUNCTION IN ALZHEIMER’S DISEASE
… our lab indicates that SYK can associate with stress granules, ribonucleoprotein particles that form in stressed cells and contain inactive translation initiation complexes. I found that microglial cell lines and primary mouse brain microglia, when stressed by exposure to sodium arsenite or …
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Role of Syk in the regulation of cytoskeleton and stress granules in breast cancer
… partners also found to be recruited to stress granules. Treatment of cells with inducers of stress granule formation leads to the recruitment of Syk to these protein-RNA complexes. This recruitment requires the phosphorylation of Syk on tyrosine and results in the phosphorylation of …
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Elucidating the Role of RanBP2/Nup358 in the Regulation of Cytokines mRNAs
… we uncovered a novel role for RanBP2 in stress granule dynamics. We showed that RanBP2 SUMOylates G3BP2, a core stress granule component, to delay stress granule formation during stress and promote their disassembly during recovery. Since many viruses target stress granules to evade …
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TDP-43 Is Directed to Stress Granules by Sorbitol, a Novel Physiological Osmotic and Oxidative Stressor
… suggests that TDP-43 associates with cytoplasmic stress granules, which are transient structures that form in response to stress. In this study, we establish sorbitol as a novel stressor that directs TDP-43 to stress granules in Hek293T cells and primary cultured glia. We quantify TDP-43 …
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The Interaction Between Host G3BP and Viral Nucleocapsid Protein Regulates SARS-CoV-2 Replication
… proteins that promote condensation to form stress granules in response to various cellular stresses, including viral infection. G3BP1/2 are prominent interactors of the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the functional …
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An omics study into the molecular impact of autosomal dominant APP and MAPT mutations on the cerebral cortex
… matter of these patients, potentially related to stress granule formation. The role of Nop58 in the brain or in tauopathies has not yet been described. The results from my dissertation provide a platform for future examination of cholesterol biosynthesis and Nop58 in tauopathy underlined by MAPT …
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An investigation of proteoforms in health and disease using peptide-level readouts
… in response to osmotic, heat, and oxidative stresses. We identify shared and stress-type specific phosphorylation signatures that align with previously reported data. We also identify phosphorylation sites on proteins known to localize to stress granules, providing a candidate list of stress …
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RNA-binding proteins mediate anti-inflammatory regulation of vascular disease
… G3BP1 by siRNA knockdown significantly reduced stress granule formation and inflammatory gene abundance in hVSMC. Microtubule inhibitors reduced SG formation in hVSMC. These results support the hypothesis that SG formation in atherosclerosis is driven by inflammation, SG may mediate the cellular …
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Functional analysis of amyotrophic lateral sclerosis mutations using Drosophila cell culture models
… Gene ontology analysis revealed that stress granule dynamics and ion channel regulation are significantly overexpressed as critical processes disrupted in TDP43 mutant expressing Drosophila S2R+ cells. This thesis enhances the understanding of genetic interactions and molecular …
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Yeast Ataxin-2 (Pbp1) Condensates Regulate TORC1 Activity and Autophagy in Response to Cellular Redox State
… protein that has earlier been implicated in stress granule formation, RNA biology, and neurodegenerative disease. However, the normal endogenous function of Pbp1 and ataxin-2 remains poorly understood. In this dissertation, I identified Pbp1 as a dedicated regulator of TORC1 signaling and …
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Parallels and Divergences in Multisystem Proteinopathy Genes: Stress Granules, Autophagy, and Myogenic Deficits
… this, we examined three areas of interest: stress granules, autophagy, and myogenesis. The currently identified roster of MSP genes have several structural and functional commonalities which fall into two categories: LC3B-intracting domain containing autophagy adaptors (SQSTM1, VCP, OPTN) …
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Molecular mechanisms underlying morphological effects of protein kinase C under normal conditions and cellular stress.
… proteins were found to localize to specific RNA-granules, formed when cells are exposed to stress. PKCalpha, rather than PKCε, was found to be the main PKC isoform found in these stress granules. We have furthermore discovered a role for PKCalpha in regulating stress granule formation.
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Regulation of cellular growth and identification of stromal gene signatures in breast cancer
… can be controlled by various mechanisms. During stress, the cell can form cytoplasmic complexes of proteins and RNAs, called stress granules, to inhibit translation of proteins unnecessary for the cell during harmful conditions and focus translation on stress-related proteins. In neuroblastoma …
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Stress-Induced Targeting of Molecular Chaperones In The Yeast Saccharomyces Cerevisiae
The eukaryotic stress response is an essential mechanism that helps protect cells from a variety of environmental stresses. Cell death can result if cells are not able to properly adapt and protect themselves against adverse stress conditions. Failure to properly deal with stress has implications …
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The NTF2L Domain Is a Date Hub That Interacts with SLiMs to Regulate Assembly of Stress Granules
… take a prototypical biomolecu- lar condensate, stress granule (SG), as an example to illustrate how the interactions between SLiMs and date hubs contribute to the interaction networks that drive biomolecular con-densation.</p> <p>We firstly confirmed that the NTF2L domain of G3BP1 protein, the …
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Mechanisms of dynein-based mRNA transport processes in human cells
… motor. As FXR1 and FXR2 are highly enriched in stress granules – large RNPs that sequester mRNAs when cells receive an insult – this thesis also explores the role of the dynein-BICD2 complex in the assembly of these structures. For this purpose, biochemical assays, as well as fixed and live cell …
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Multi-color visualization and quantification of single RNA translation and HIV-1 programmed ribosomal frameshifting in living cells
… RNAs interactions with ribonucleoprotein (RNP) granules during cellular stress. NCT was used to image real-time single RNAs, their translational output, and RNA-granule interactions during stress. Although translating mRNAs only interact with RNP granules dynamically, non-translating mRNAs can …
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SYK promotes TGF-beta-induced P-body clearance in breast cancer cells through the enhancement of autophagy
… with processing bodies (P-bodies), mRNP granules that are associated with mRNA degradation. These interactions led me to hypothesize that Syk co-localizes with P-bodies. I found that Syk co-localizes with P-bodies in HEK293T cells. Moreover, I show tyrosine phosphorylation occurring in …
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