Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"Stress Fiber"”.
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Stress Fiber Formation and Migration Are Novel PGR-Dependent Pathways Initiated in the Granulosa Cells of the Ovulating Follicle
… ‘formation of actin filaments’ and ‘formation of stress fibers’ as primary pathways. In contrast, RNA-seq analysis of the ovarian stromal compartment found minimal PGR-dependent regulation of stromal genes. It was then established that in response to ovulatory stimulus, thick actin stress fibers …
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Tetraspanin KAI1/CD82 inhibits cell migration-related cellular events via reorganizing actin network
… by defects in actin cortical network and stress fiber formations. Notably, actin polymerization was attenuated by KAI1/CD82. Although Rac1 activity was diminished upon KAI1/CD82 expression, Rac1 could not rescue lamellipodia formation because Rac1 activity is not required for this process …
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Molecular and Cellular Mechanisms of LIM Protein Mechanotransduction
… force-activated binding events could mediate stress fiber repair: a paradigmatic example of cytoskeletal mechanotransduction mediated by the LIM protein zyxin. While cellular studies have uncovered the molecular components involved in stress fiber repair, the precise molecular mechanism by …
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Alternative splicing of the zebrafish myosin phosphatase targeting subunit, MYPT1, produces a novel isoform
… complex with Protein Phosphatase 1 β (PP1β) via stress fiber assay. TV202 was also shown to be localized in the cytoplasm, enriched in a paranuclear manner. TV202Δ was found the be localized inside the nucleus. It was also found that TV202 was zygotically, but not maternally, expressed during …
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Differential Regulation of Phosphatidylinositol 4-Phosphate 5 Kinase Beta during Hypertonic and Oxidative Stress
… laboratory found that hypertonic and oxidative stress increases and decreases PIP2 levels, respectively. In this thesis, I sought to understand how these two different types of stresses alter PIP2 by examining the effects on the phosphatidylinositol 5-phosphate 4 kinases that generate PIP2. …
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Influence of rearrangement of actin cytoskeleton on the overall material properties of ATDC5 cells during chondrogenesis
… of the cytoskeletal structure (from stress-fiber to punctate appearance) was observed. This data was then used to construct a Finite Element model of cell. The cell was considered as a composite matrix and the actin as the embedded fibers. Radial deformations of the cell (difference …
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Characterization of zebrafish zipper-interacting protein kinase
… ZIPK, but not rodent ZIPK were able to increase stress fiber formation and myosin light chain-2 (MLC-2) phosphorylation in vitro. Human and zebrafish ZIPK underwent nucleocytoplasmic shuttling without PAR-4 interaction, unlike rodent ZIPK, which required PAR-4 for nuclear exit. Unlike human ZIPK, …
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Effect of Anti-fibrotic Drug (Pirfenidone) on TGF-β Signaling Pathway in Ocular Fibrotic Disorders
… typical phenotypic changes such as formation of stress fiber running parallel to the long axis of cells, and enhanced migration and production of extracellular matrix components such as collagen type I and fibronectin. This fibroblast-like phenotype induced by TGF-β1 was significantly inhibited …
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Lithograph regulation of cellular mechanical properties c by Tsu-Te Judith Su.
… between these mechanical parameters and actin stress fiber distribution. It was discovered that the fractal dimension is a weak inverse function of cell adhesion area but that there is a significant change in fractal dimension between patterned and control cells which can freely spread to their …
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Interactions of Fibroblast with Cytotoxic and Invasive Strains of Pseudomonas aeruginosa on ECM Mimicking Fibers
… cell-pathogen interactions on highly aligned nanofibers of varied spacing and diameter, which mimic the fibroblast deposited ECM and dictate fibroblast morphology. We discovered that the cytotoxic strain of P. aeruginosa induced significantly shorter fibroblast death times. Furthermore, two modes …
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The Effects of Acetylenic Tricyclic Bis-(Cyano Enone) on Cell Migration
… 374 of actin, inhibits actin polymerization and stress fiber formation. These findings were applied to a model of epithelial-to-mesenchymal transition, a precursor event to metastasis, where I determined TBE-31 was able to inhibit the crucial rearrangement of cortical actin to form actin stress …
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The Transformation of Human Retinal Pigment Epithelial Cells
… type of transformation of RPE cells with stress fiber formation. TGFβ induces Snail, α5integrin and FN-EDA expressions, but decreases Slug expression. Furthermore, TGFβ induces myofibroblast markers (αSMA and CTGF), although vitreous suppresses their expressions. Vitreous also …
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Regulation of RhoA Activation and Actin Reorganization by Diacylglycerol Kinase
… RhoA signalling downstream and disrupted stress fibers. Moreover, DGKz loss resulted in decreased stress fiber formation following the expression of a constitutively active RhoA mutant, suggesting it is also important for RhoA function following activation. This is consistent with the …
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Structural Stiffness Gradient along a Single Nanofiber and Associated Single Cell Response
… spreading, migration, cytoskeleton arrangement (stress fiber and focal adhesion distribution) and differentiation. In this study using our recently described non-electrospinning fiber manufacturing platform, customized scaffolds of suspended nanofibers are developed to study single cell behavior …
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Cancer Associated Fibroblast Derived Angiogenic Factor Mfap5 In Ovarian Cancer Progression
… and permeability via focal adhesion and stress fiber formation by activating the calcium dependent FAK/ERK/LPP signaling pathway. Finally, monoclonal anti-MFAP5 antibodies were developed. These antibodies demonstrated inhibitory effects on tumor growth and improved paclitaxel …
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NMR AS A TOOL TO QUANTITATIVELY CHARACTERIZE MOLECULAR MECHANISMS GOVERNING ACTIN DYNAMICS AND RICE LATERAL ROOT DEVELOPMENT
… important for cellular shape, cell motility and stress fiber repair. This study presented a model for a mechanism of action where phosphorylation can act as a stoichiometric switch and can explain the importance of the phosphorylation cycle in the localization of VASP by Zyxin. The second …
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Synthetic Peptide Ligand Mimetics and Tumor Cell Motility
… of the actin cytoskeleton resulting in increased stress fiber formation and actin colocalization with cortactin at the cell membrane. HYD1 interacts with a6 and a3 integrin subunits and elevates laminin-5 dependent intracellular signals including focal adhesion kinase, mitogen activated protein …
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Synovial Fibrosis in Osteoarthritis of the Knee: Mechanism of Development and Potential Therapeutic Targets
… smooth muscle actin (a-SMA) upregulation and stress fiber formation. Transforming growth factor-β (TGF-b) plays an important role in mediating the pathogenesis of synovial fibrosis. Synoviocytes produce proteoglycan-4 (PRG4; lubricin), a heavily glycosylated mucinous glycoprotein, and …
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The Effect of Microenvironmental Cues on Adipocyte Cytoskeletal Remodeling
… adipocyte maturation and inducing actin stress fiber formation facilitated by myocardin-related transcription factor A (MRTF-A/MKL1) nuclear translocation. The second aim explores the effects of mechanical unloading (simulated microgravity) on key adipocyte functions and actin …
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Mechanisms and Functional Consequences of Cardiac Remodeling: Role of Myocardial Rac1 and Vascular Profilin1 Genes
… of transgenic mice favoring F-actin induces stress fiber formation and plays an important role in vascular hypertrophy by inducing internal mechanical stress, leading to vascular remodeling and hypertension by the time the profilin-1 mice were 6-month old. Mild hypertension clinically can …
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