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Showing 1 to 20 of 40 for “"Streptomyces coelicolor"”.
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Inhibition of Hsp90 in Streptomyces coelicolor
… the effect of Hsp90 inhibition on the bacteria Streptomyces coelicolor. These changes were studied by growing S. coelicolor in the presence of Hsp90 inhibitors, extracting the growth media, and subjecting the extractions to HPLC and MS analysis. It has been found that reproducibility is …
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MarR Family Transcriptional Regulators from Streptomyces coelicolor
… methyltransferase regulator) and PecS in Streptomyces coelicolor were studied to investigate their potential role in this bacterium. In Streptomyces coelicolor, the gene (SCO3133), which encodes TamR, is oriented divergently from the tam gene, which encodes trans-aconitate …
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Nucleoid-Associated Proteins of Streptomyces coelicolor: Discovery and Functions
… A method for surveying the proteome of the S. coelicolor nucleoid was developed which generated a list of 25 proteins with a high probability of being NAPs. This list included known NAPs such as HupA, HupS, Lsr2 and sIHF and the known global regulators CRP and BldD, as well as a number of …
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The regulatory role of MtrA and NsrR in streptomyces coelicolor
Streptomyces coelicolor inhabits a harsh soil environment, which it must adapt and respond to; therefore how S. coelicolor senses and responds to its environment is of the utmost importance. This project addresses two types of regulation system: the three-component system MtrAB-LpqB and the …
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Biophysical analysis of MarR family transcription factors from Streptomyces coelicolor
… are 42 of these MFRs present in the genome of Streptomyces coelicolor, a prolific producer of secondary metabolites, the majority of which are uncharacterized. In this work, the crystal structures of three of these MFRs from Streptomyces coelicolor, Sco3914, Sco4122 and Sco5413, have been …
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Functional and mathematical analysis of the glyoxylate shunt in Streptomyces coelicolor
Streptomyces coelicolor is the model organism for the genus Streptomyces, which produces many bioactive secondary metabolites with clinical applications. Based on work done in Escherichia coli, the glyoxylate shunt was thought to be the main anapleurotic pathway in S. coelicolor during growth on …
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Gene-enzyme relations affecting tryptophan biosynthesis in Streptomyces coelicolor A3(2)
… least two separate regions on the chromosome of Streptomyces coelicolor A3(2). Tryptophan-requiring mutants capable of utilizing indole mapped counterclockwise of hisC9 between hisC9 and proAl. Trp mutants unable to use indole in place of tryptophan mapped clockwise of hisC9 between hisC9 and …
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Understanding the Genome-Wide Response of Streptomyces coelicolor to the Glycopeptide Antibiotic Teicoplanin
… for providing resistance to teicoplanin in S. coelicolor, which is instead mediated mainly by the elusive membrane protein, VanJ. This study further characterised VanJ by comparing the transcriptomes of a wt S. coelicolor A3(2) M600 strain and an isogenic ΔvanJ knock-out mutant after exposing …
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The dual-targeted membrane assembly of the <i>Streptomyces coelicolor</i> Rieske protein
… problem. Previous work has shown that the <i>Streptomyces coelicolor</i> Rieske protein is dual targeted to the cytoplasmic membrane since it requires both the Sec and Tat export pathways for correct insertion.<br/><br/>The aim of this study was to investigate this dual-targeting mechanism and …
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Genetic and phenotypic analyses of auxotrophs requiring aromatic amino acids and vitamins in Streptomyces coelicolor
Five mutants of Streptomyces coelicolor having auxotrophic requirement for aromatic amino acids were mapped relative to closely linked genetic markers. Based on four-point reciprocal crosses mutants were found to map in at lea.st two locations. One mutant mapped between proA and hisC while three …
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Efecto de la concentración citosólica de cobre en la germinación y el metabolismo secundario en Streptomyces coelicolor
Estudiamos la cepa de S. coelicolor mutada en los genes SCO2730/2731 (chaperona de cobre, copZ / transportador de cobre, copA). Esta cepa muestra retraso en germinación, crecimiento y esporulación, pero también un aumento notable en producción de metabolitos secundarios y un metabolismo secundario …
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Los operadores SCO1897-1901 y SCO2102-2013 modulan la diferenciación y el metabolismo secundario de Streptomyces coelicolor
Streptomyces es una bacteria no patógena del suelo caracterizada por un ciclo de vida complejo que incluye procesos de muerte celular programada, diferenciación y esporulación. Desde el punto de vista aplicado, Streptomyces produce dos tercios de los antibióticos y otros compuestos bioactivos de …
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