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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 145 for “"Streptavidin"”.
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Streptavidin-cytokine fusion proteins for use as adjuvants in cancer vaccines
… production and characterisation of recombinant streptavidin-cytokine fusion proteins. Immunoadjuvants can augment the weak or non-existent antitumour response of a patient with cancer to immunotherapy. The response of breast cancer patients to cancer vaccines directed against tumour associated …
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A potential route to hydrogel multifunctionalization utilizing encapsulation of acrylate-conjugated streptavidin
… biologically active hydrogels utilizing the high streptavidin-biotin binding affinity. Streptavidin was conjugated to acryl-PEG-N-hydroxysuccinimide, a commercially available molecule that allows chemical binding to poly(ethylene glycol) (PEG) diacrylate and dextran acrylate hydrogels. Such gels …
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Production and preliminary characterization of a fusion protein comprising streptavidin and a cellulose-binding domain
… or immobilized on cellulose (Ong et al., 1989b). Streptavidin, a protein produced by Streptomyces avidinii, binds to the water-soluble vitamin, D-biotin, with remarkably high affinity. The strong and specific biotin-binding affinity of streptavidin offers a variety of applications in biotechnology …
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Engineering streptavidin and its target ligands with both infinite binding affinity and reversible binding capability
… purification of proteins, it would be ideal for streptavidin to have extremely tight binding to its target ligands (biotin and its binding tags) and at the same time to retain the feature of reversible binding capability. To achieve this objective, a streptavidin mutein (SAVSBPM32) was engineered …
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Quantum dot encoded magnetic beads for multiplexed fluorescence biosensing
… between quantum dot-biotin and quantum dot-streptavidin conjugates to create constructs on the magnetic bead surface. The development of both of these immobilisation strategies was characterisation using X-ray photoelectron spectroscopy and fluorescence spectroscopy of immobilised quantum …
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Design and development of a site specific protein patterning technique for use in a microfluidic antibody separation device
… the patterns were. The conjugation of streptavidin, streptavidin-FITC, NeutrAvidin, single domain protein L and multidomain protein L to silicon surfaces, upon reaction with UANHS, were investigated. Fluorescently labelled probes that associated with the protein motifs were used to …
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Attachment mechanisms of a novel, targeted, lipid-based, ultrasound contrast agent
… assessed for microbubble attachment include a streptavidin-biotin mechanism, electrostatic mechanism and antibody targeting. The microbubble has been optimized for use with 40MHz IVUS through an investigation into the effect of various production methods on the echogenicity of the agent. …
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Studies fo the microstructure of self-assembling peptides : effects of chemical cross-linking and growth characterization
… in combination with the observation that streptavidin and biotin can be used to increase the peptide gel's modulus as measured by rheometery that raises the prospect of controlling cell function by control of matrix physical properties. Atomic force microscope (AFM) was used to reveal the …
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Development of Protein Based PCBP2 siRNA Nanocomplex for Liver Fibrosis Therapy
… step for the silencing activity of siRNAs. Using streptavidin as the nanocomplex core, PCBP2 siRNA was delivered to HSC-T6 (hepatic stellate) cells. Intracellular fate of the nanocomplex components and PCBP2 siRNA was monitored. Fluorescent probes were tagged with the siRNA, protamine and …
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Preparation of Heparin Surface for Quantification of Fibroblast Growth Factor-2 (FGF-2) Binding Using Surface Plasmon Resonance (SPR)
… (SPR) instrument. The heparin was attached via streptavidin-biotin linkage where the streptavidin was covalently coupled to the mSAM and biotinylated heparin bound to it. These chips were then used to quantify the interactions of fibroblast growth factor-2 (FGF-2) with the surface bound heparin. …
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How Kinesin-1 Deals With Roadblocks: Biophysical Description and Nanotechnological Application
… first stop and then detach when they encounter a streptavidin obstacle on their path along the microtubule. Consequently, the deceleration of streptavidin coated microtubules in gliding assays could be attributed to an obstruction of kinesin-1's path on the microtubule rather than to …
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Cyaninfarbstoffe als Fluoreszenzsonden in biomimetischen und biologischen Systemen : Fluoreszenz-Korrelations-Spektroskopie und Fluoreszenzanisotropie-Untersuchungen
… im jeweiligen Modellsystem. Das Biotin-Streptavidin-System wurde als Modellsystem für die Untersuchung von Protein-Ligand-Wechselwirkungen verwendet, da der Bindungsmechanismus weitgehend aufgeklärt ist. Nach Bindung der Farbstoffe an Streptavidin wurde eine erhebliche Veränderung in den …
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Reactive and soft landing of polyatomic gas-phase ions on plasma-treated metal surfaces
… function, as demonstrated for trypsin and streptavidin. The majority of trypsin ions soft-landed at hyperthermal kinetic energies retain enzymatic activity. The landed streptavidin can be washed into solution where they show affinity to biotin. The layer of streptavidin monomer that is …
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Development of an optical cavity-based biosensor for point-of-care diagnostics.
… the limit-of-detection (LOD) of the OCB, streptavidin and C-reactive protein (CRP) detections were performed. The optical cavity design was optimized further, and the surface functionalization process was improved. The polymer swelling was effectively utilized to fine-tune the cavity width …
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Development of a PDMS Based Micro Total Analysis System for Rapid Biomolecule Detection
… determination of mixing efficiency based on the streptavidin-biotin conjugation reaction between biotin comjugated fluorescent microbeads and streptavidin conjugated paramagnetic microbeads, followed by fluorescence measurements. Based on the performance test results, one of the micromixers was …
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Synthetic creation of a chemotactic system via utilization of magnetically actuated microrobotic walkers
… the walkers. The receptor-ligand pair of biotin-streptavidin was utilized due to the extremely strong binding affinity of the pair. The presence of free biotin binding sites on the surface was required to obtain chemotactic motion as these binding sites modulated walker velocity. The walkers …
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Novel Precursors for Polymer-Protein-Conjugate Synthesis via Reversible Addition-Fragmentation Chain Transfer Polymerization
… for subsequent conjugation to the protein streptavidin. Sulfhydryl-terminated PNIPAAm-b-PAA was conjugated to the streptavidin mutant S139C using a bismaleimide cross-linker and also direct conjugation via disulfide linkage. Both conjugations were successful and proceeded with more than 50 …
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