Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Strep-tag"”.
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Development of bispecific filamentous bacteriophages for the generation of a novel automated screening system based on phage display technology
… DNA sequence was fused to geneIII and the Strep-tag® encoding DNA to geneIX. Functional coexpression of the resulting scFvCWP/pIII- and Strep-tag®/pIX-fusion proteins as well as their incorporation into correctly assembled bispecific phage particles was verified by phage ELISA and …
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Elucidating the role of Mitoferrin (Mfrn), Iron Regulatory Proteins (IRP1 and IRP2) and Hephaestin (Heph) in Iron Metabolism by tagSNP and Protein-Protein Interaction (PPI) analysis
… and Heph in iron homeostasis. Analysis of 18 tagSNPs in the Mfrn gene was carried out in an Asian-Caucasian population to establish any correlation between the Mfrn tagSNPs, haemoglobin levels and birth weight in the presence of covariates such as sex of the fetus, gestational age and mother's …
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Konstruktion eines bakteriellen Systems zum Export von Coenzym B<sub>12</sub>
… der Gene die Sequenz für einen His6- oder Strep-Tag angefügt. Western-Blot-Analysen zeigten nur eine geringe Produktion des sigTorA-IF-Fusionsproteins, dagegen wurde das sigTorA-MutB Fusionsprotein in großen Mengen in verschiedenen Enterobakterien wie z.B. Citrobacter freundii und …
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Interazioni tra le glicoproteine D, B, H ed L critiche per la fusione indotta dal virus Herpes simplex
… HSV-HHV8 gB chimeras with gH or gD fused to the strep-tag. The gB sequence between aa residues 219-360 was identified as putative region of interaction with gH or critical to the interaction.
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Investigating chlorite dismutase biocatalyst reuse using columns
… a chlorite dismutase biocatalyst modified with a strep tag; this reuse system allows the biocatalyst to bind to coated resin beads that can be reused in a column system. Chlorite dismutase treats chlorite to chloride and oxygen. Activity tests were conducted in batch for the purified and attached …
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Identifizierung und Charakterisierung der Isoflavon-umsetzenden Enzyme aus dem humanen Darmbakterium Slackia isoflavoniconvertens
… Reduktasen wurden diese genetisch an ein Strep-tag fusioniert und mittels Affinitätschromatographie gereinigt. Die übrigen durch Daidzein induzierten Proteine IfcA, IfcBC und IfcE wurden ebenfalls in E. coli exprimiert und als Strep-Fusionsproteine gereinigt. Vergleichende Aktivitätstests …
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The tyrosine regulated DAHP synthase and the biosynthetic pathway of aromatic amino acids in Saccharomyces cerevisiae
… durch PHA2 kodierte Prephenat-Dehydratase als Strep tag® Fusionsprotein exprimiert und aufgereinigt. Das Enzym zeigte eine Michaelis-Menten-Kinetik mit einem kcat von 16 s-1. Ein einziger Aminosäureaustausch in der NSRP-Region (Asp308Glu) der Domäne, die Ähnlichkeit zur regulatorischen …
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Molecular basis oh herpes simplex virus entry into the cell and retargeting of the viral tropism for the design of oncolytic herpesviruses
… and purified the heterodimer by means of ONE-STrEP-tag system by IBA. With respect to biological function, the purified heterodimer is capable: • of reacting to antibodies that recognize conformation dependent epitopes and neutralize virion infectivity; • of binding a variety cells at cell …
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Understanding the biochemical properties and physiological function of the protein syncollin
… Syncollin was expressed with various epitope tags (including His, GST and Strep) in bacteria, insect cells and mammalian cells. The best results were obtained by expressing syncollin bearing a double-Strep tag at its C terminus (syncollin-Strep) in mammalian (tsA-201) cells and purifying the …
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Production and characterization of the recombinant wheat chitinase Wch1 and generation of chitin-specific antibodies
… fused at the N-terminal end of both a his6 tag and a Strep-tag II and as a C-terminal fusion with the maltose binding protein (MBP). Furthermore, the cDNA gene was cloned into plant expression vectors, enabling accumulation of the recombinant protein in the plant cell apoplast (Wch1-apo) and …
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Determination of fatty acid synthesis intermediates in escherichia coli and bacillus subtilis
… growth. The method primarily relies on an inert strep-tagged ACP which is purified using a two-step purification strategy utilizing volatile buffers of low pH in order to preserve the thioester bond between the acyl group and ACP. Samples obtained via this method were extremely pure and free of …