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Showing 1 to 7 of 7 for “"Strain-promoted azide-alkyne cycloaddition"”.
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Chemical and enzymatic tools to study proteins in their native cellular environment
… that we have engineered to accept and ligate an azide functional handle onto a 13-amino acid ḺplA a̲cceptor peptide (LAP). Subsequent derivatization of the azide with fluorophores functionalized with cyclooctyne via strain-promoted azide-alkyne cycloaddition allowed us to target many bright and …
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4D Control of Tissue Development and Blood Vasculature using Photo-mediated Chemistries
… poly(ethylene glycol) (PEG) hydrogels formed via strain-promoted azide-alkyne cycloaddition (SPAAC). In this section, material and cellular variables are optimized to both create functional cardiac tissues from hPSC-derived cardiomyocytes (CM) using PEG hydrogels as well as first maintain and then …
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Functionalization of Peptide Nucleic Acids via post-synthetic click chemistry
… PNA MBs were prepared by incorporation of azide-containing monomers into the oligomer by automatic solid-phase peptide synthesis and subsequent derivatization with pyrene moieties by copper-catalyzed azide-alkyne cycloaddition (CuAAC) produced the functional MBs. Two pyrene-based …
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Bioorthogonal Tethering of Drug Fragments to Engineered G Protein-Coupled Receptors
… was more specific and efficient than the strain promoted azide-alkyne cycloaddition reaction assessed. GCE was then employed to site-specifically introduce one of three different reactive ncAAs in CCR5 near an allosteric binding site for the drug maraviroc (mvc). Molecular dynamics …
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A multiplexed approach for quantitative profiling of the translatome using bioorthogonal non-canonical amino acids
… (Aha), a methionine analog that contains an azide functional group, such that proteins synthesized for the duration of the pulse incorporate Aha. By coupling pulsed stable isotope labeling of amino acids in cell culture (pSILAC) and Aha metabolic labeling of newly synthesized proteins with …
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Strain-promoted Stapled Peptides for Inhibiting Protein-protein Interactions
… is peptide stapling, which involves constraining a short peptide into its native alpha-helical form by forming a covalent link between two of its amino acid side-chains. The Sondheimer dialkyne reagent has previously been used in strain-promoted double-click cycloadditions with …
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DEVELOPMENT OF CRISPR-CAS IMMOBILIZATION SRATEGIES FOR THE FABRICATION OF A BIOSENSING PLATFORM
… click chemistry reactions, the so called Strain-promoted azide-alkyne cycloaddition (SPAAC) between a dibenzocyclooctyne (DBCO) and azide groups, and the reaction between hydrazine and aldehyde. This latter reaction allowed the formation of a hydrogel with minimal interference with the …