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Showing 1 to 20 of 98 for “"Stopped-flow"”.
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Computer interfaced stopped-flow kinetics
Stopped-flow kinetics is a method in which two reactants are forced into a mixing chamber and their flow abruptly stopped. The progress of the reaction is monitored at an observation window, a short distance from the chamber along the flow path. Traditionally, data from the stopped-flow has been …
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Automated Analysis by Stopped-Flow Methodology
Extensions to the stopped-flow analyzer have been developed that demonstrate capabilities which offer the stopped-flow method as an attractive alternative for dedicated chemical analysis. The instrumentation has been designed to expand the versatility of the stopped-flow method, exploiting its …
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Automated Clinical/analytical Methodologies Developed for a Stopped-Flow Spectrophotometric System
Made available in DSpace on 2014-12-13T20:10:25Z (GMT). No. of bitstreams: 1 7820983.pdf: 7729717 bytes, checksum: d08b7f2b6a85c850a4a76aaa271ee1dc (MD5) Previous issue date: 1978
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A Stopped-Flow Analyzer for Kinetic Methods Development - Automated Implementation of Guggenheim's Methods
… single-beam spectrophotometer into a versatile stopped-flow kinetic analyzer. A relatively inexpensive microcomputer system serves as a dedicated controller/data processor for the instrument. The design employed features preservation of the optical configuration and geometry of the original …
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Clinical-Scale Hyperpolarization of 129Xe and 131Xe via Stopped-Flow Spin Exchange Optical Pumping
… keeping polarizations high, particularly in a “stopped-flow” polarizer environment. This dissertation is divided into eight main chapters. The first chapter is written to provide a historical context for the SEOP field and summarize the evolutionary stages that have led to current methodologies. …
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Development and Characterization of a Stopped-Flow-Bypass Analysis System With Applications To Biochemical Measurements
A new apparatus called Bypass Trapped Flow Analysis System (ByT-FAS) is described. A properly designed ByT-FAS gives an analyst the ability to use analyte sample volumes of 10 to 200 μL [or more] and reagent volumes of approximately the same size. The sample and reagent are injected into their …
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Investigation of the Structure and Kinetics of Heme-Containing Proteins Using Magnetic Circular Dichroism and Rapid-Scan, Stopped-Flow Spectroscopy
… (UV-Vis) electronic absorption and rapid-scan, stopped-flow spectroscopies were utilized to determine reaction mechanisms and kinetics data. Due to the chromophore properties of heme-containing proteins, magnetic circular dichroism (MCD) spectrophotometry was used to determine structural aspects …
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An Automated Spectrophotometric Stopped-Flow System for Reaction-Rate Measurements and Application to the Rapid Quantitative Determination of Phosphate in Soil and Water Samples
Made available in DSpace on 2014-12-10T23:01:27Z (GMT). No. of bitstreams: 1 7309916.pdf: 8180279 bytes, checksum: d7a6d2dfcd2901443949146ca66290d2 (MD5) Previous issue date: 1972
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Vízkezelési eljárásokban képződő toxikus vegyületek analízise kromatográfiás módszerekkel
… keresztül. Az N-monoklóraminosav képződése a stopped-flow készülék holtideje alatt lejátszódik, így már csak abszorbancia ugrásként tudtuk detektálni. Az N,N-diklóraminosav megjelenését, majd bomlását stopped-flow módszerrel tudtuk nyomon követni. Hosszabb időskálán a hipoklórossavat …
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Early Events in the Folding of the Protein Ubiquitin
… technique and by low temperature stopped flow mixing. In the case of the T-Jump, initially cold denatured protein solution is rapidly heated with a 20 nsec heating pulse. The subsequent refolding is monitored by time resolved fluorescence lifetime and spectrum. In the case of …
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Kinetics and Mechanism of Syn, Alpha' Lithiation of Tertiary Benzamides
… reso- nance, freezing point depression and stopped-flow infrared spectros- copy indicates the reaction proceeds with rapid initial formation of tetrameric complexes followed by rate determining deprotonation. Model studies using N,N,N',N'-tetramethylethylenediamine (TMEDA)
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Investigation of the Chemiluminescent Reaction Between Lucigenin and Organic Reductants and Its Application as a Detector for High Pressure Liquid Chromatography
… was carried out in three distinct steps: (1) stopped flow investigation of the reaction parameters; (2) development of a flow injection system for investigation, specifically the development of a chemiluminescence detector for HPLC, and; (3) application of the chemiluminescence detector to the …
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Part I. Studies of the substitution and formation reactions of rhenium carbonyl o-semiquinone compounds. Part II. Molecular mechanics studies of the steric interactions in chromium pentacarbonyl phosphine compounds
… with P(n-Bu)$\sb3$ was studied by stopped-flow methods. The observed kinetics data shows considerable variability, both within and among different sets of runs. The results are best explained by an electron-transfer catalyzed chain mechanism.
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Mechanistic Studies of Bimolecular Electron Transfer Self-Exchange Rates of Ruthenium Ammine Pyridyl Complexes Measured by NMR Line Broadening Techniques
… These observations matche well with recent stopped-flow work by Mehmood on a similar set of related pseudo-self exchange reactions. Kinetics of the (NH<sub>3</sub>)<sub>5</sub>Ru<sup>II/III </sup>(3-trifluoromethylpyridine)<sup>2+/3+</sup> self-exchange reactions with addition of various …
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Unusual Electron Transfer Rate Effects due to Pyridyl Ring Substituents and Trace Group VIII b Hexacyano Complexes as Added Salts
… of various salts added were studied by using the stopped-flow kinetic spectroscopy. Marcus theory rate <em>vs</em>. driving force plots yielded distinct families of lines depending on whether a 3- or 4- phenylpyridine ligand was present, and reactions of 4-phenylpyridine were in all cases the …
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The study of [beta]-cyclodextrin interactions with sugars using "inhibition kinetics" and the bromination of pyrimidine derivatives
… solution were monitored using a single beam stopped-flow spectrophotometer. Both substrates exhibit saturation kinetics and 1:1 binding with [beta]-CD, and the rates of hydrolysis are retarded by the cyclodextrin, in agreement with earlier studies. The retarded rates of hydrolysis in the …
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Bioinorganic hydrocarbon oxidation : mechanistic and kinetic studies of the soluble methane monooxygenase from Methylococcus capsulates (bath)
… and Substrates by Rapid Freeze- Quench and Stopped-Flow Spectroscopy -- Chapter 5. Oxidation of Radical Clock Substrate Probes by the Soluble Methane Monooxygenase System -- Chapter 6. Tritiated Chiral Alkanes as Probes for the Mechanism of Hydroxylation by the Soluble Methane Monooxygenase.
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Mechanistic studies of the class I ribonucleotide reductases from S. cerevisiae and E. coli.
… of the class I E. coli RNR is investigated using stopped-flow UV-visible spectroscopy and rapid chemical quench methods. These studies have led to a model in which the rate determining step in catalysis is a physical step prior to proton-coupled electron transfer (PCET), nucleotide reduction, and …
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Kinetik der Vesikelbildung in katanionischen Tensidsystemen
… Systeme. Diese wurde mit Hilfe von Stopped Flow Messungen mit unterschiedlichen Detektionsmethoden verfolgt. Es konnte festgestellt werden, dass es sich bei der Vesikelbildung um einen mehrstufigen Prozess handelt. Bei den salzhaltigen Mischungen zeigte sich, dass die …
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Mechanistic Studies of Salt Effects on Bimolecular Electron-Transfer Reactions of Pentaamine Ruthenium Pyridyl Complexes Studied by 19F NMR Line-Broadening and T2 Spin-Echo Techniques
… kinetic salt effects. In agreement with earlier stopped-flow work, addition of alkali-metal fluoride salts increased the rate of ET between the like-charged redox reactants in good quantitative agreement with the quantitative predictions of the Debye-Huckle-Bronsted theory of ion atmosphere …
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