Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"Stimulated Emission Depletion (STED) Microscopy"”.
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Stimulated Emission Depletion (STED) Microscopy and Pacific Orange Dye Optimization For H9C2 Cox-1 Imaging Via Indirect Immunocytochemistry
<p>The fundamental barrier of traditional microscopy has always been the Abbe limit. Diffraction has served to limit image production and microscopic investigation on the sub-cellular level, greatly hindering microbiology and other forms of study. Stimulated Emission Depletion microscopy is one of …
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Dual-Colour STED-microscopy on the Nanoscale
Stimulated emission depletion (STED) microscopy was the first concept for breaking Abbe's diffraction barrier in optical far-field microscopy verified in biological applications. However, the theoretically infinite resolution was limited due to photobleaching of the fluorescent species. In this …
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Development and application of correlative STED and AFM to investigate neuronal cells
… migration a technique combining atomic force microscopy (AFM) with stimulated emission depletion (STED) microscopy was developed. First a custom STED microscope was developed. To facilitate the design of this system the theoretical performance of a range of STED techniques (cw-STED, time-gated …
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Adaptive optics stimulated emission depletion microscope for thick sample imaging
Over the past few decades, fluorescence microscopy has proven to become the most widely used imaging technique in the field of life sciences. Unfortunately, all classical optical microscopy techniques have one thing in common: their resolution is limited by the diffraction. Thankfully, due to the …
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Diabetes and the Female Heart
… Finally, I employed super-resolution Stimulated Emission Depletion (STED) microscopy and automated image analysis pipelines to quantify key ECC proteins organisation: transverse tubules (T tubule), ryanodine receptor type 2 (RyR2) clusters, and filamentous actin (F-actin). Results …
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Development of the multi-scale dynamic modeling techniques for multi-modal brain recordings
… (~200 nm), we need an in-vivo super-resolution microscopy to achieve our goal. As a super-resolution imaging method, stimulated emission depletion (STED) microscopy has unraveled fine intracellular structures and provided insights into nanoscale organizations in cells. Although image resolution …
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Investigating New Guaiazulenes and Diketopyrropyrroles for Photonic Applications
… under acidic conditions, including absorption, emission, quantum yield, and optical band gap patterns. These results were exploited in the design of a photosensitive polymeric system with potential application in the field of three dimensional (3D) optical data storage (ODS). Chapter 2 describes …