Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 104 for “"Stem cell differentiation"”.
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H4K16 acetylation during embryonic stem cell differentiation
… component of the doseage compensation system. It is also unique amongst histone modifications in that it has been directly associated with chromatin decompaction. H4K16ac has been linked to development through its Histone Acetyltransferase, MOF. Deletion of MOF in mice leads to mass …
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Capturing Complex Microenvironments for Directed Stem Cell Differentiation
… overcome the limitations associated with adult cell sources, which are often restricted by supply or compromised by disease, mesenchymal stem cells (MSCs) have emerged as potential candidates for vascular tissue engineering. While evidence suggests the roles of several factors influencing MSC …
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Engineered N-cadherin substrates for stem cell differentiation
… Certain success was made to modulate neural differentiation of stem cells using soluble factors and cell adhesion matrix properties. These differentiation studies are often dependent on the density of cells plated on the substrate, which implies that there is an important role of …
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Understanding the Dynamics of Embryonic Stem Cell Differentiation
The two defining features of mouse embryonic stem (ES) cells are self-renewal and naive pluripotency, the ability to give rise to all cell lineages in the adult body. In addition to being a unique and interesting cell type, pluripotent ES cells have demonstrated their potential for continued …
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Regulated Histone H3 Proteolysis During Mouse Embryonic Stem Cell Differentiation
… chromatin often lead to dynamic changes in many cellular processes, including development and differentiation, by affecting the accessibility of the genomic information stored in the DNA. The cell uses many different mechanisms to regulate chromatin in order to establish, maintain, and propagate …
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The regulation of mouse embryonic stem cell differentiation by Nrf2
Embryonic stem (ES) cell maintenance and differentiation are dynamic processes controlled by various intrinsic and extrinsic factors. Identifying these factors will enhance the understanding about developmental process and improve the application of stem cells in clinic. Previous studies highlight …
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Role of Endoplasmic Reticulum Stress in Trophoblast Stem Cell Differentiation
… of endoplasmic reticulum (ER) stress on the differentiation of trophoblast stem cells (TSCs), placental development, and subsequent pregnancy outcome. To address this question, I first used an in vitro mouse TSC model. Morphological observations suggest that upon ER stress, TSCs lose stemness …
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Engineering Tools to Promote and Characterize Wnt-Mediated Stem Cell Differentiation
… we constructed a kinetic model consisting of a system of ordinary differential equations. We fit this model to empirical time course data derived from Western blots of HEK293T cells treated with Wnt. From this fit we were able to gain insights into how the intracellular levels of the Wnt pathway …
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The Role of Snail Homolog 1 in Embryonic Stem Cell Differentiation
During gastrulation, epiblast cells undergo an epithelial-to-mesenchymal transition: EMT) as they ingress through the primitive streak and form mesoderm. To better understand the molecular pathways of EMT during this developmental transition, we developed a model system utilizing mouse embryonic …
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Stem cell differentiation study: Statistical analysis and application to microarray data
… and data mining procedures for modeling stem cell genes during the differentiation process, (2) To present biological interpretations of "sternness" genes based on time-course expression patterns and genetic networks. Stem cells have been of much interest over the past few years. Up to …
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Neural stem cell differentiation in collagen scaffolds for retinal tissue engineering
Rat neural stem cells (NSCs) were cultured in monolayer or in porous collagen scaffolds and exposed to neurogenic or non-neurogenic medium to determine the effects on neural differentiation and neurite growth. Nestin, [beta]III-tubulin, and GFAP expression were determined using immunofluorescent …
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Complementary Strategies to Promote Mesenchymal Stem Cell Differentiation for Ligament Tissue Engineering
… graft choices for repair of ACL disruptions. The cell-based approach to construct engineered ligament grafts presented herein involves the culture of mesenchymal stem cells (MSC) on biodegradable, fibrous polymeric scaffolds to promote tissue formation. Multipotent MSCs are advantageous because of …
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The Functions of Setd5 and Mir-221 In Embryonic Stem Cell Differentiation
<p>Embryonic stem cells (ESCs) are a widely used model system to study cellular differentiation because of their pluripotent characteristics, and ESC differentiation is an epigenetic process. In an effort to identify a new epigenetic factor that is required for ESC differentiation, the function of …
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The role of Polycomb-mediated epigenetic regulation in embryonic stem cell differentiation
During mammalian development, a single founding cell must produce all of the different types of cells in the adult organism. What are the regulatory mechanisms required to coordinate the necessary gene expression networks for this process? Polycomb group (PcG) proteins are epigenetic regulators …
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Elucidating Cause-Effect Relationships between Extracellular Matrix Signaling and Mesenchymal Stem Cell Differentiation
Mesenchymal stem cell (MSC) differentiation is known to be influenced by a range of environmental stimuli. MSC-based bone regeneration strategies would benefit from the identification of scaffold material properties which intrinsically promote osteoblast lineage progression. The aim of this work …
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P120-Catenin Regulates Rest and Corest, and Modulates Mouse Embryonic Stem Cell Differentiation
… studied to determine their contributions to stem cell biology, but less is known about p120-catenin in the nuclear compartment. P120 is developmentally required as a consequence of its biochemical and functional interactions with cadherins, small-GTPases and transcriptional regulators. We …
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Targeted mesenchymal stem cell differentiation from induced pluripotent stem cells for therapeutic application
<p>Over the past decade, mesenchymal stem cells have increasingly gained interest as a cell source for the treatment of a variety of diseases. This is because they play several roles in tissue homeostasis and regeneration, such as altering the immune response to limit inflammation, aiding tissue …
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The Role of Nitric Oxide and Peroxynitrite in Human Embryonic Stem Cell Differentiation
Human embryonic stem cells (hESCs) have the potential to grow into almost all types of cells in the human body. This special property makes hESC invaluable for the restoration of tissue function after damage from different diseases like Parkinson's disease, Alzheimer’s disease, stroke, and …
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Effects of low oxygen culture on pluripotent stem cell differentiation and teratoma formation
Pluripotent stem cells (PSC) hold promise for the study of embryonic development and the treatment of many diseases. Most pluripotent cell research is performed in incubators with a gas-phase oxygen partial pressure (p02) of 142 mmHg. However, embryonic cells in early development are exposed to a …
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Genetic regulation of neuronal stem cell differentiation: a single-cell sequencing study in planarians
Cellular specialisation is incredibly prominent in the nervous system, where even in a single brain region, individual neurons may differ deeply in their molecular, anatomical, and functional features. Platyhelminthes like Schmidtea mediterranea continuously turn over all their tissues, including …
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