Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"Staurosporine"”.
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STAUROSPORINE-INDUCED APOPTOSIS/PROGRAMMED CELL DEATH
… breakdown which could commit the cell to die. Staurosporine, an established 'universal' apoptogen was used to induce apoptosis in human Chang liver cells and human KB epidermoid carcinoma cells. While staurosporine is known to induce interphase specific cell cycle arrest at either G1/S or G2/M …
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Structural studies of rebeccamycin, staurosporine, and violacein biosynthetic enzymes
… bisindole natural products rebeccamycin, staurosporine, and violacein from the common starting material L-tryptophan involves shared enzymatic transformations. However, the pathways diverge at two steps, each involving a reactive, bisindole intermediate. We have taken a structural approach …
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On the origins of enzyme inhibitor selectivity and promiscuity: a case study of protein kinase binding to staurosporine
… and I show that the tetrahydropyran ring of staurosporine causes induced-fit of the glycine rich loop. I apply multiple linear regression to select distances measured between the distinctive parts of residues which correlate with the binding constants. This method allows me to understand the …
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Genetic analysis of thymocyte apoptosis
… immature mouse thymocytes by ceramide analogues, staurosporine, and Fas stimulation, through diverse signal transduction pathways, ultimately resulting in caspase protease activation. Glucocorticoids (GC), ceramide analogues, and staurosporine pathways are inhibited by the Bcl-2-class of apoptosis …
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Heat shock protein 27 inhibition of apoptosis in human breast cancer cells
… with apoptotic inducers such as Sodium Butyrate, Staurosporine, Vitamin E Succinate and Cycloheximide. The cells that constitutively expressed Hsp 27 had a higher growth in the presence of Cycloheximide and Vitamin E Succinate and also exhibited lesser apoptosis in the presence of Sodium Butyrate …
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Apoptosis Pathways: Presence and significance in Ejaculated Human Spermatozoa
… beads. Fractions were divided into groups: staurosporine, anti-Fas antibody, and hydrogen peroxide treated and control. Direct enzymatic measurement of caspase activity, flow cytometric evaluation of phosphatidylserine translocation, immunoblots, and immunocytochemistry were used in this …
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TGF-β1-induced Chloride Channel Activity and Migration of Human Eosinophils
… inhibitor rottlerin, the general PKC inhibitor staurosporine, and the chloride channel inhibitors 4,4’-diisothiocyanatostilbene-2,2’-disulfonic acid (DIDS) and 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB). Protein expression of the chloride channel (CLC) family of chloride channels, PKC …
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Synthesis of Marine Chemicals and Derivatives as Potential Anti-Cancer Drugs.
… inhibition of prostate cancer cell growth using staurosporine a a positive control. All three compounds have shown significant inhibition of prostate cancer cell growth. Compound <b>9</b> is yet to be evaluated.</p>
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Contribution of LEDGF/p75 to Prostate Cancer Chemoresistance
… by the classical apoptosis inducers TRAIL and staurosporine. This selectivity might be related to LEDGF/p75’s ability to protect cells against insults that induce ROS generation, since we observed that DTX but not TRAIL and staurosporine generated ROS. To better understand the mechanism by …
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AUTOANTIGENS IN PARKINSON'S DISEASE
… also demonstrated to be neuroprotective against staurosporine-induced neurotoxicity in human dopaminergic neurons derived from induced pluripotent stem cells. Our clinical and neuro-immunological studies thus highlight the potential of the STIP1 co-chaperone as an endogenous neuroprotective agent …
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Inactivation of the JAK-STAT Pathway
… signaling from the IFN-γ receptor (imposed by staurosporine treatment) previously activated Stat1 disappeared within 30-60 minutes, implying continuous generation and removal of tyrosine phosphorylated molecules during extended IFN-γ treatment. The proteasome, induced inhibitory proteins and …
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Development of a Human Lung Cancer Cytochrome C-GFP Reporter Cell Line to Study Anti-cancer Drug Responses in Apoptosis Pathways
… different apoptosis-inducing reagents; TRAIL, Staurosporine, and Tarceva. The result from this study illustrated that the dynamic release of cytochrome C-GFP is dependent on the cell types and doses. We have also developed a system to investigate the anti-cancer drugs response in a sequence of …
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Investigation of the absorption pathways of some poorly absorbed drugs using human intestinal (CAC0-2) cell monolayers
… (CHPP) and N-benzoyl-staurosporine (NBS), and the polypeptide, human calcitonin. Lanthanum ions are proposed as a paracellular pathway inhibitor and tested with D-mannitol permeability and transepithelial electrical resistance measurements. The effect La3+ has on the …
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Methods and technologies for high-throughput and high-content small animal screening
… This screen identifies the kinase inhibitor staurosporine as a strong inhibitor of neural regeneration, and does so in a concentration and neuronal cell type-specific manner. Finally, we present a simple device for immobilizing C. elegans inside standard microtiter plates that is compatible …
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G-Triple Chemiluminescent DNAZyme Based Biosensor for the Detection of Calcium(II) Ions in Solution and the Biological Functionality of Kinase Enzymes and the Rationale for their Employment as Promising Drug Targets, Substrate Specific Inhibition of P38alpha
… Kinase A (PKA) and the pan-kinase inhibitor Staurosporine with the final goal of evaluating the ability of rooperol analogues to inhibit the protein kinase p38a. p38a is a Mitogen Activated Protein Kinase (MAPK) which when dysregulated can cause diseases such as autoimmune disorders, …
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Effect of cellular factors on the generation of beta-amyloid.
… the N-terminal construct sensitises cells to staurosporine-induced apoptosis. TEM images from cells expressing the fusion protein reveals numerous phagosomes and mutilaminar bodies that fit the profile seen for the blob-like aggregates in terms of dimension, number and general morphology. …
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Windows in the clock: An in vitro study of melatonin action and signal transduction in the suprachiasmatic circadian pacemaker
… to alter pacemaker function. The PKC inhibitor, staurosporine, completely blocks both melatonin and TPA phase-advances at each sensitive period, strengthening this hypothesis. However, melatonin did not alter forskolin-stimulated cAMP levels, nor SCN IP$\sb3$ levels, at CT 6, 10, or 23. These …
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The roles of redox active cofactors in catalysis : structural studies of iron sulfur cluster and flavin dependent enzymes
… study of FAD in the context of rebeccamycin and staurosporine biosynthesis has yielded another role for this cofactor in the enzyme StaC. A homolog of this enzyme, RebC, uses its FAD cofactor in the oxidation of 7-carboxy-K252c. StaC also uses 7-carboxy-K252 as a substrate, but its reaction does …
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Large-scale transgenesis and nerve regeneration in C. elegans
… we found that the PKC kinase inhibitor staurosporine strongly modulates regeneration in a concentration- and neuronal type-specific manner. Two structurally unrelated PKC inhibitors produce similar effects. We further show that regeneration is significantly enhanced by the PKC activator …
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